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@article{Jolly:2019:10.1016/j.bios.2018.09.006,
author = {Jolly, P and Rainbow, J and Regoutz, A and Estrela, P and Moschou, D},
doi = {10.1016/j.bios.2018.09.006},
journal = {Biosensors and Bioelectronics},
pages = {244--250},
title = {A PNA-based Lab-on-PCB diagnostic platform for rapid and high sensitivity DNA quantification.},
url = {http://dx.doi.org/10.1016/j.bios.2018.09.006},
volume = {123},
year = {2019}
}

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TY  - JOUR
AB  - We report the development of a Lab-on-PCB DNA diagnostic platform, exploiting peptide nucleic acid (PNA) sequences as probes. The study demonstrates the optimization and characterization of two commercial PCB manufacturing gold electroplating processes for biosensing applications. Using an optimized ratio of PNA with a spacer molecule (MCH), the lowest limit of detection (LoD) to date for PCB-based DNA biosensors of 57 fM is reported. The study also showcases a fully integrated Lab-on-PCB microsystem designed for rapid detection, which employs PCB-integrated sample delivery, achieving DNA quantification in the 0.1-100pM range for 5μL samples analyzed within 5min under continuous flow. The demonstrated biosensor proves the capability of PCB-based DNA biosensors for high sensitivity and paves the way for their integration in Lab-on-PCB DNA diagnostic microsystems.
AU  - Jolly,P
AU  - Rainbow,J
AU  - Regoutz,A
AU  - Estrela,P
AU  - Moschou,D
DO  - 10.1016/j.bios.2018.09.006
EP  - 250
PY  - 2019///
SN  - 0956-5663
SP  - 244
TI  - A PNA-based Lab-on-PCB diagnostic platform for rapid and high sensitivity DNA quantification.
T2  - Biosensors and Bioelectronics
UR  - http://dx.doi.org/10.1016/j.bios.2018.09.006
UR  - https://www.ncbi.nlm.nih.gov/pubmed/30243847
UR  - http://hdl.handle.net/10044/1/64068
VL  - 123
ER  -

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