Imperial College London
Alternate

Professor Bill Rutherford FRS

Faculty of Natural Sciences, Department of Life Sciences

Chair in Biochemistry of Solar Energy
 
 
 
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Contact

 

+44 (0)20 7594 5329a.rutherford Website

 
 
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Location

 

702Sir Ernst Chain BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Shevela:2020:10.1021/acs.biochem.0c00208,
author = {Shevela, D and Do, H-N and Fantuzzi, A and Rutherford, AW and Messinger, J},
doi = {10.1021/acs.biochem.0c00208},
journal = {Biochemistry},
pages = {2442--2449},
title = {Bicarbonate-Mediated CO<sub>2</sub> Formation on Both Sides of Photosystem II.},
url = {http://dx.doi.org/10.1021/acs.biochem.0c00208},
volume = {59},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The effect of bicarbonate (HCO<sub>3</sub><sup>-</sup>) on photosystem II (PSII) activity was discovered in the 1950s, but only recently have its molecular mechanisms begun to be clarified. Two chemical mechanisms have been proposed. One is for the electron-donor side, in which mobile HCO<sub>3</sub><sup>-</sup> enhances and possibly regulates water oxidation by acting as proton acceptor, after which it dissociates into CO<sub>2</sub> and H<sub>2</sub>O. The other is for the electron-acceptor side, in which (i) reduction of the Q<sub>A</sub> quinone leads to the release of HCO<sub>3</sub><sup>-</sup> from its binding site on the non-heme iron and (ii) the <i>E</i><sub>m</sub> potential of the Q<sub>A</sub>/Q<sub>A</sub><sup>•-</sup> couple increases when HCO<sub>3</sub><sup>-</sup> dissociates. This suggested a protective/regulatory role of HCO<sub>3</sub><sup>-</sup> as it is known that increasing the <i>E</i><sub>m</sub> of Q<sub>A</sub> decreases the extent of back-reaction-associated photodamage. Here we demonstrate, using plant thylakoids, that time-resolved membrane-inlet mass spectrometry together with <sup>13</sup>C isotope labeling of HCO<sub>3</sub><sup>-</sup> allows donor- and acceptor-side formation of CO<sub>2</sub> by PSII to be demonstrated and distinguished, which opens the door for future studies of the importance of both mechanisms under <i>in vivo</i> conditions.
AU  - Shevela,D
AU  - Do,H-N
AU  - Fantuzzi,A
AU  - Rutherford,AW
AU  - Messinger,J
DO  - 10.1021/acs.biochem.0c00208
EP  - 2449
PY  - 2020///
SN  - 0006-2960
SP  - 2442
TI  - Bicarbonate-Mediated CO<sub>2</sub> Formation on Both Sides of Photosystem II.
T2  - Biochemistry
UR  - http://dx.doi.org/10.1021/acs.biochem.0c00208
VL  - 59
ER  -
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