Imperial College London
Alternate

Professor Dame Amanda Fisher

Faculty of Medicine, Institute of Clinical Sciences

Visiting Professor
 
 
 
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Contact

 

amanda.fisher

 
 
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Assistant

 

Ms Alessandra Lisini +44 (0)20 3313 8236

 
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Location

 

CRB (Clinical Research Building)Hammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Su:2004:10.1038/ng1351,
author = {Su, RC and Brown, KE and Saaber, S and Fisher, AG and Merkenschlager, M and Smale, ST},
doi = {10.1038/ng1351},
journal = {Nat Genet},
pages = {502--506},
title = {Dynamic assembly of silent chromatin during thymocyte maturation.},
url = {http://dx.doi.org/10.1038/ng1351},
volume = {36},
year = {2004}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Considerable knowledge has been gained from temporal analyses of molecular events culminating in gene activation, but technical hurdles have hindered comparable studies of gene silencing. Here we describe the temporal assembly of silent chromatin at the mouse terminal transferase gene (Dntt), which is silenced and repositioned to pericentromeric heterochromatin during thymocyte maturation. Silencing was nucleated at the Dntt promoter by the ordered deacetylation of histone H3 at Lys9 (H3-Lys9), loss of methylation at H3-Lys4 and acquisition of methylation at H3-Lys9, followed by bidirectional spreading of each event. Deacetylation at H3-Lys9 coincided with pericentromeric repositioning, and neither of these early events required de novo protein synthesis. CpG methylation increased primarily in mature T cells that had left the thymus. A transformed thymocyte line supported reversible inactivation of Dntt without repositioning. In these cells, histone modification changes were nucleated at the promoter but did not spread. These results provide a foundation for elucidating the mechanisms of silent chromatin assembly during development.
AU  - Su,RC
AU  - Brown,KE
AU  - Saaber,S
AU  - Fisher,AG
AU  - Merkenschlager,M
AU  - Smale,ST
DO  - 10.1038/ng1351
EP  - 506
PY  - 2004///
SN  - 1061-4036
SP  - 502
TI  - Dynamic assembly of silent chromatin during thymocyte maturation.
T2  - Nat Genet
UR  - http://dx.doi.org/10.1038/ng1351
UR  - http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=15098035&query_hl=1
VL  - 36
ER  -
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