Imperial College London
Alternate

Professor Dame Amanda Fisher

Faculty of Medicine, Institute of Clinical Sciences

Visiting Professor
 
 
 
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Contact

 

amanda.fisher

 
 
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Assistant

 

Ms Alessandra Lisini +44 (0)20 3313 8236

 
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Location

 

CRB (Clinical Research Building)Hammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Pereira:2008:10.1371/journal.pgen.1000170,
author = {Pereira, CF and Terranova, R and Ryan, NK and Santos, J and Morris, KJ and Cui, W and Merkenschlager, M and Fisher, AG},
doi = {10.1371/journal.pgen.1000170},
journal = {PLoS Genetics},
title = {Heterokaryon-based reprogramming of human B lymphocytes for pluripotency requires Oct4 but not Sox2},
url = {http://dx.doi.org/10.1371/journal.pgen.1000170},
volume = {4},
year = {2008}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Differentiated cells can be reprogrammed through the formation of heterokaryons and hybrid cells when fused withembryonic stem (ES) cells. Here, we provide evidence that conversion of human B-lymphocytes towards a multipotent stateis initiated much more rapidly than previously thought, occurring in transient heterokaryons before nuclear fusion and celldivision. Interestingly, reprogramming of human lymphocytes by mouse ES cells elicits the expression of a human ESspecific gene profile, in which markers of human ES cells are expressed (hSSEA4, hFGF receptors and ligands), but markersthat are specific to mouse ES cells are not (e.g., Bmp4 and LIF receptor). Using genetically engineered mouse ES cells, wedemonstrate that successful reprogramming of human lymphocytes is independent of Sox2, a factor thought to be requiredfor induced pluripotent stem (iPS) cells. In contrast, there is a distinct requirement for Oct4 in the establishment but not themaintenance of the reprogrammed state. Experimental heterokaryons, therefore, offer a powerful approach to trace thecontribution of individual factors to the reprogramming of human somatic cells towards a multipotent state.
AU  - Pereira,CF
AU  - Terranova,R
AU  - Ryan,NK
AU  - Santos,J
AU  - Morris,KJ
AU  - Cui,W
AU  - Merkenschlager,M
AU  - Fisher,AG
DO  - 10.1371/journal.pgen.1000170
PY  - 2008///
SN  - 1553-7390
TI  - Heterokaryon-based reprogramming of human B lymphocytes for pluripotency requires Oct4 but not Sox2
T2  - PLoS Genetics
UR  - http://dx.doi.org/10.1371/journal.pgen.1000170
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000260411200024&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/71581
VL  - 4
ER  -
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