Imperial College London
Alternate

Professor Dame Amanda Fisher

Faculty of Medicine, Institute of Clinical Sciences

Visiting Professor
 
 
 
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Contact

 

amanda.fisher

 
 
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Assistant

 

Ms Alessandra Lisini +44 (0)20 3313 8236

 
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Location

 

CRB (Clinical Research Building)Hammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Cobb:2005:10.1084/jem.20050572,
author = {Cobb, BS and Nesterova, TB and Thompson, E and Hertweck, A and O'Connor, E and Godwin, J and Wilson, CB and Brockdorff, N and Fisher, AG and Smale, ST and Merkenschlager, M},
doi = {10.1084/jem.20050572},
journal = {Journal of Experimental Medicine},
pages = {1367--1373},
title = {T cell lineage choice and differentiation in the absence of the RNase III enzyme dicer},
url = {http://dx.doi.org/10.1084/jem.20050572},
volume = {201},
year = {2005}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The ribonuclease III enzyme Dicer is essential for the processing of micro-RNAs (miRNAs) and small interfering RNAs (siRNAs) from double-stranded RNA precursors. miRNAs and siRNAs regulate chromatin structure, gene transcription, mRNA stability, and translation in a wide range of organisms. To provide a model system to explore the role of Dicer-generated RNAs in the differentiation of mammalian cells in vivo, we have generated a conditional Dicer allele. Deletion of Dicer at an early stage of T cell development compromised the survival of alphabeta lineage cells, whereas the numbers of gammadelta-expressing thymocytes were not affected. In developing thymocytes, Dicer was not required for the maintenance of transcriptional silencing at pericentromeric satellite sequences (constitutive heterochromatin), the maintenance of DNA methylation and X chromosome inactivation in female cells (facultative heterochromatin), and the stable shutdown of a developmentally regulated gene (developmentally regulated gene silencing). Most remarkably, given that one third of mammalian mRNAs are putative miRNA targets, Dicer seems to be dispensable for CD4/8 lineage commitment, a process in which epigenetic regulation of lineage choice has been well documented. Thus, although Dicer seems to be critical for the development of the early embryo, it may have limited impact on the implementation of some lineage-specific gene expression programs.
AU  - Cobb,BS
AU  - Nesterova,TB
AU  - Thompson,E
AU  - Hertweck,A
AU  - O'Connor,E
AU  - Godwin,J
AU  - Wilson,CB
AU  - Brockdorff,N
AU  - Fisher,AG
AU  - Smale,ST
AU  - Merkenschlager,M
DO  - 10.1084/jem.20050572
EP  - 1373
PY  - 2005///
SN  - 0022-1007
SP  - 1367
TI  - T cell lineage choice and differentiation in the absence of the RNase III enzyme dicer
T2  - Journal of Experimental Medicine
UR  - http://dx.doi.org/10.1084/jem.20050572
UR  - http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=15867090&query_hl=1
UR  - http://hdl.handle.net/10044/1/71594
VL  - 201
ER  -
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