Imperial College London


Faculty of EngineeringDepartment of Bioengineering

Research Associate







ACE ExtensionSouth Kensington Campus






Research assistant in Chemical Engineering, Department of Chemical Engineering and Chemical Technology, Imperial College London, UK


MSc in Advance Chemical Engineering, Department of Chemical Engineering and Chemical Technology, Imperial College London, UK

MSc Thesis: Adsorption and Anaerobic Bioregeneration in the treatment of Olive Oil Mill Wastewater Polyphenols with Powdered Activated Carbon 


Diploma in Chemical Engineering, National Technical University of Athens, Greece

Diploma thesis project: Bioethanol Production from Cheese-whey by different Fusarium Oxysporum strains 


The BTEX group of aromatic compounds consist of the pollutants benzene, toluene, ethylbenzene and xylene.  These pollutants can be found in petroleum, pesticides, plastics and synthetic fibers which constitute major sources of environmental pollution. Bioremediation is a physical, environmental-friendly and cost-efficient solution.

Pseudomonas putida mt-2 is able to degrade toluene and m-xylene through the chromosomal and TOL plasmid metabolic pathways. The transformation of the pollutant takes place sequentially by a series of enzymes produced by the genes of the microorganism as soon as the pollutant enters the bacterial system. Up-stream regulation plays an imperative role on the expression of pollutant’s degradation and biomass growth rate. Therefore microbial growth kinetics are re-established with the aid of the advances in molecular biology and a mechanistic profile of the kinetics is obtained.

Research Interests:

Molecular biology

Modelling/Optimization of bacterial cell culture systems



Tsipa A, Koutinas M, Vernardis SI, et al., 2017, The impact of succinate trace on pWW0 and ortho-cleavage pathway transcription in Pseudomonas putida mt-2 during toluene biodegradation, Bioresource Technology, Vol:234, ISSN:0960-8524, Pages:397-405

Tsipa A, Koutinas M, Pistikopoulos EN, et al., 2016, Transcriptional kinetics of the cross-talk between the ortho-cleavage and TOL pathways of toluene biodegradation in Pseudomonas putida mt-2, Journal of Biotechnology, Vol:228, ISSN:0168-1656, Pages:112-123


Tsipa A, Koutinas M, Pistikopoulos EN, et al., 2015, Prediction of double substrate microbial growth kinetics through transcriptional regulation: An integrated experimental/modelling approach on Pseudomonas putida mt-2, Pages:763-768

Tsipa A, Koutinas M, Mantalaris A, et al., 2014, Development of a new paradigm in biochemical engineering: Predicting the genetic regulation of aromatic pollutants degradation, Pages:550-554

More Publications