Imperial College London
Portrait Picture

ProfessorBorisLenhard

Faculty of Medicine, Institute of Clinical Sciences

Professor of Computational Biology
 
 
 
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Contact

 

+44 (0)20 7594 0911b.lenhard Website

 
 
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Assistant

 

Mr Alastair Douglas Ivor Williams +44 (0)20 3313 4318

 
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Location

 

6.12CLMS BuildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Beltran:2021:10.15252/embj.2020105564,
author = {Beltran, T and Pahita, E and Ghosh, S and Lenhard, B and Sarkies, P},
doi = {10.15252/embj.2020105564},
journal = {The EMBO Journal},
pages = {1--17},
title = {Integrator is recruited to promoter-proximally paused RNA Pol II to generate Caenorhabditis elegans piRNA precursors},
url = {http://dx.doi.org/10.15252/embj.2020105564},
volume = {40},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Piwiinteracting RNAs (piRNAs) play key roles in germline development and genome defence in metazoans. In C. elegans, piRNAs are transcribed from > 15,000 discrete genomic loci by RNA polymerase II (Pol II), resulting in 28 nt shortcapped piRNA precursors. Here, we investigate transcription termination at piRNA loci. We show that the Integrator complex, which terminates snRNA transcription, is recruited to piRNA loci. Moreover, we demonstrate that the catalytic activity of Integrator cleaves nascent capped piRNA precursors associated with promoterproximal Pol II, resulting in termination of transcription. Loss of Integrator activity, however, does not result in transcriptional readthrough at the majority of piRNA loci. Taken together, our results draw new parallels between snRNA and piRNA biogenesis in nematodes and provide evidence of a role for the Integrator complex as a terminator of promoterproximal RNA polymerase II during piRNA biogenesis.
AU  - Beltran,T
AU  - Pahita,E
AU  - Ghosh,S
AU  - Lenhard,B
AU  - Sarkies,P
DO  - 10.15252/embj.2020105564
EP  - 17
PY  - 2021///
SN  - 0261-4189
SP  - 1
TI  - Integrator is recruited to promoter-proximally paused RNA Pol II to generate Caenorhabditis elegans piRNA precursors
T2  - The EMBO Journal
UR  - http://dx.doi.org/10.15252/embj.2020105564
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000600042700001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://www.embopress.org/doi/full/10.15252/embj.2020105564
UR  - http://hdl.handle.net/10044/1/86152
VL  - 40
ER  -
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