Imperial College London
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ProfessorBorisLenhard

Faculty of Medicine, Institute of Clinical Sciences

Professor of Computational Biology
 
 
 
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Contact

 

+44 (0)20 7594 0911b.lenhard Website

 
 
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Assistant

 

Mr Alastair Douglas Ivor Williams +44 (0)20 3313 4318

 
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Location

 

6.12CLMS BuildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Stadhouders:2015:10.1038/ncomms9893,
author = {Stadhouders, R and Cico, A and Stephen, T and Thongjuea, S and Kolovos, P and Baymaz, HI and Yu, X and Demmers, J and Bezstarosti, K and Maas, A and Barroca, V and Kockx, C and Ozgur, Z and van, Ijcken W and Arcangeli, M-L and Andrieu-Soler, C and Lenhard, B and Grosveld, F and Soler, E},
doi = {10.1038/ncomms9893},
journal = {Nature Communications},
title = {Control of developmentally primed erythroid genes by combinatorial co-repressor actions},
url = {http://dx.doi.org/10.1038/ncomms9893},
volume = {6},
year = {2015}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - How transcription factors (TFs) cooperate within large protein complexes to allow rapid modulation of gene expression during development is still largely unknown. Here we show that the key haematopoietic LIM-domain-binding protein-1 (LDB1) TF complex contains several activator and repressor components that together maintain an erythroid-specific gene expression programme primed for rapid activation until differentiation is induced. A combination of proteomics, functional genomics and in vivo studies presented here identifies known and novel co-repressors, most notably the ETO2 and IRF2BP2 proteins, involved in maintaining this primed state. The ETO2–IRF2BP2 axis, interacting with the NCOR1/SMRT co-repressor complex, suppresses the expression of the vast majority of archetypical erythroid genes and pathways until its decommissioning at the onset of terminal erythroid differentiation. Our experiments demonstrate that multimeric regulatory complexes feature a dynamic interplay between activating and repressing components that determines lineage-specific gene expression and cellular differentiation.
AU  - Stadhouders,R
AU  - Cico,A
AU  - Stephen,T
AU  - Thongjuea,S
AU  - Kolovos,P
AU  - Baymaz,HI
AU  - Yu,X
AU  - Demmers,J
AU  - Bezstarosti,K
AU  - Maas,A
AU  - Barroca,V
AU  - Kockx,C
AU  - Ozgur,Z
AU  - van,Ijcken W
AU  - Arcangeli,M-L
AU  - Andrieu-Soler,C
AU  - Lenhard,B
AU  - Grosveld,F
AU  - Soler,E
DO  - 10.1038/ncomms9893
PY  - 2015///
SN  - 2041-1723
TI  - Control of developmentally primed erythroid genes by combinatorial co-repressor actions
T2  - Nature Communications
UR  - http://dx.doi.org/10.1038/ncomms9893
UR  - http://hdl.handle.net/10044/1/29891
VL  - 6
ER  -
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