Imperial College London

DrBrianRobertson

Faculty of MedicineDepartment of Medicine

Reader in Systems Microbiology
 
 
 
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Contact

 

+44 (0)20 7594 3198b.robertson

 
 
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Assistant

 

Ms Sophie Kemp +44 (0)20 7594 3199

 
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Location

 

3.41Flowers buildingSouth Kensington Campus

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Summary

 

Publications

Publication Type
Year
to

108 results found

Blokpoel MCJ, Murphy HN, O'Toole R, Wiles S, Runn ESC, Stewart GR, Young DB, Robertson BDet al., 2005, Tetracycline-inducible gene regulation in mycobacteria, NUCLEIC ACIDS RESEARCH, Vol: 33, ISSN: 0305-1048

JOURNAL ARTICLE

Murphy HN, Stewart GR, Mischenko VV, Apt AS, Harris R, McAlister MSB, Driscoll PC, Young DB, Robertson BDet al., 2005, The OtsAB pathway is essential for trehalose biosynthesis in Mycobacterium tuberculosis, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 280, Pages: 14524-14529, ISSN: 0021-9258

JOURNAL ARTICLE

Stewart GR, Newton SM, Wilkinson KA, Humphreys IR, Murphy HN, Robertson BD, Wilkinson RJ, Young DBet al., 2005, The stress-responsive chaperone alpha-crystallin 2 is required for pathogenesis of Mycobacterium tuberculosis, MOLECULAR MICROBIOLOGY, Vol: 55, Pages: 1127-1137, ISSN: 0950-382X

JOURNAL ARTICLE

Stewart GR, Patel J, Robertson BD, Rae A, Young DBet al., 2005, Mycobacterial mutants with defective control of phagosomal acidification, PLOS PATHOGENS, Vol: 1, Pages: 269-278, ISSN: 1553-7366

JOURNAL ARTICLE

Wiles S, Ferguson K, Robertson B, Young Det al., 2005, Optimisation of conditions for the use of a novel bioluminescent reporter system in Mycobacterium spp, Bioluminescence and Chemiluminescence: Progress and Perspectives, Pages: 543-546, ISBN: 9789812702203

BOOK CHAPTER

Wiles S, Ferguson K, Stefanidou M, Young DB, Robertson BDet al., 2005, Alternative luciferase for monitoring bacterial cells under adverse conditions, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Vol: 71, Pages: 3427-3432, ISSN: 0099-2240

JOURNAL ARTICLE

Papatheodorou I, Sergot M, Randall M, Stewart GR, Robertson BDet al., 2004, Visualization of microarray results to assist interpretation, TUBERCULOSIS, Vol: 84, Pages: 275-281, ISSN: 1472-9792

JOURNAL ARTICLE

Stewart GR, Robertson BD, Young DB, 2004, Analysis of the function of mycobacterial DnaJ proteins by overexpression and microarray profiling, TUBERCULOSIS, Vol: 84, Pages: 180-187, ISSN: 1472-9792

JOURNAL ARTICLE

Stewart GR, Robertson BD, Young DB, 2003, Tuberculosis: A problem with persistence, NATURE REVIEWS MICROBIOLOGY, Vol: 1, Pages: 97-105, ISSN: 1740-1526

JOURNAL ARTICLE

Harrison OB, Robertson BD, Faust SN, Jepson MA, Goldin RD, Levin M, Heyderman RSet al., 2002, Analysis of pathogen-host cell interactions in purpura fulminans: Expression of capsule, type IV pili, and PorA by Neisseria meningitidis in vivo, INFECTION AND IMMUNITY, Vol: 70, Pages: 5193-5201, ISSN: 0019-9567

JOURNAL ARTICLE

Young DB, Robertson BD, 2002, Immune intervention in tuberculosis, Immunology of Infectious Diseases, Pages: 439-451, ISBN: 9781555812140

BOOK CHAPTER

Ho TBL, Robertson BD, Rhee JT, Young DB, Small PM, Shaw RJet al., 2001, Are deletions in the PLCD locus associated with variation in infectivity in clinical isolates of Mycobacterium tuberculosis?, THORAX, Vol: 56, Pages: 56-56, ISSN: 0040-6376

JOURNAL ARTICLE

Young D, Robertson B, 2001, Genomics: Leprosy - A degenerative disease of the genome, Current Biology, Vol: 11, ISSN: 0960-9822

Analysis of the genome of the leprosy bacillus uncovers evidence of extensive deletion and inactivation of genes. Secluded in a specialised niche, it has discarded much of its genetic heritage, though retaining just enough to be a major human pathogen.

JOURNAL ARTICLE

Young D, Robertson B, 2001, Genomics: leprosy - a degenerative disease of the genome., Curr Biol, Vol: 11, Pages: R381-R383, ISSN: 0960-9822

Analysis of the genome of the leprosy bacillus uncovers evidence of extensive deletion and inactivation of genes. Secluded in a specialised niche, it has discarded much of its genetic heritage, though retaining just enough to be a major human pathogen.

JOURNAL ARTICLE

De Smet KAL, Weston A, Brown IN, Young DB, Robertson BDet al., 2000, Three pathways for trehalose biosynthesis in mycobacteria, MICROBIOLOGY-UK, Vol: 146, Pages: 199-208, ISSN: 1350-0872

JOURNAL ARTICLE

Devyatyrova-Johnson M, Rees IH, Robertson BD, Turner MW, Klein NJ, Jack DLet al., 2000, The lipopolysaccharide structures of Salmonella enterica serovar Typhimurium and Neisseria gonorrhoeae determine the attachment of human mannose-binding lectin to intact organisms, INFECTION AND IMMUNITY, Vol: 68, Pages: 3894-3899, ISSN: 0019-9567

JOURNAL ARTICLE

Dockrell HM, Brahmbhatt S, Robertson BD, Britton S, Fruth U, Gebre N, Hunegnaw M, Hussain R, Manadhar R, Murrillo L, Pessolani MCV, Roche P, Salgado JL, Sampaio E, Shahid F, Thole JER, Young DBet al., 2000, Diagnostic assays for leprosy based on T-cell epitopes, Workshop on Leprosy Research at the New Millennium, Publisher: LEPRA, Pages: S55-S58, ISSN: 0305-7518

CONFERENCE PAPER

Dockrell HM, Brahmbhatt S, Robertson BD, Britton S, Fruth U, Gebre N, Hunegnaw M, Hussain R, Manandhar R, Murillo L, Pessolani MCV, Roche P, Salgado JL, Sampaio E, Shahid F, Thole JER, Young DBet al., 2000, A postgenomic approach to identification of Mycobacterium leprae-specific peptides as T-cell reagents, INFECTION AND IMMUNITY, Vol: 68, Pages: 5846-5855, ISSN: 0019-9567

JOURNAL ARTICLE

Herrmann JL, Delahay R, Gallagher A, Robertson B, Young Det al., 2000, Analysis of post-translational modification of mycobacterial proteins using a cassette expression system., FEBS Lett, Vol: 473, Pages: 358-362, ISSN: 0014-5793

A recombinant expression system was developed to analyse sequence determinants involved in O-glycosylation of proteins in mycobacteria. By expressing peptide sequences corresponding to known glycosylation sites within a chimeric lipoprotein construct, amino acids flanking modified threonine residues were found to have an important influence on glycosylation. The expression system was used to screen mycobacterial sequences selected using a neural network (NetOglyc) trained on eukaryotic O-glycoproteins. Evidence of glycosylation was obtained for eight of 11 proteins tested. The results suggest that sites involved in O-glycosylation of mycobacterial and eukaryotic proteins share similar structural features.

JOURNAL ARTICLE

Ho TBL, Robertson BD, Michael Taylor G, Shaw R, Young DBet al., 2000, Comparison of Mycobacterium tuberculosis genomes reveals frequent deletions in a 20 kb variable region in clinical isolates, Yeast, Vol: 17, Pages: 272-282, ISSN: 0749-503X

The Alycobactcrium tuberculosis complex is associated with a remarkably low level of structural gene polymorphism. As part of a search for alternative forms of genetic variation that may act as a source of biological diversity in M. tuberculosis, we have identified a region of the genome that is highly variable amongst a panel of unrelated clinical isolates. Fifteen of 24 isolates examined contained one or more copies of the M. tuberculosis-specific IS6110 insertion element within this 20 kb variable region. In nine of the isolates, including the laboratory-passaged strain H37Rv, gcnomic deletions were identified, resulting in loss of between two and 13 genes. In each case, deletions were associated with the presence of a copy of the IS6110 element. Absence of flanking tri- or tetra-nuclcotidc repeats identified homologous recombination between adjacent IS6110 elements as the most likely mechanism of the deletion events. IS61I10 insertion into hotspots within the genome of M. tuberculosis provides a mechanism for generation of genetic diversity involving a high frequency of insertions and deletions. Copyright ©2000 John Wiley & Sons, Ltd.

JOURNAL ARTICLE

Ho TBL, Robertson BD, Taylor GM, Shaw RJ, Young DBet al., 2000, Comparison of Mycobacterium tuberculosis genomes reveals frequent deletions in a 20 kb variable region in clinical isolates, YEAST, Vol: 17, Pages: 272-282, ISSN: 0749-503X

JOURNAL ARTICLE

Young DB, Robertson BD, 1999, TB vaccines: global solutions for global problems., Science, Vol: 284, Pages: 1479-1480, ISSN: 0036-8075

JOURNAL ARTICLE

Young DB, Robertson BD, 1999, Microbiology - TB vaccines: Global solutions for global problems, SCIENCE, Vol: 284, Pages: 1479-1480, ISSN: 0036-8075

JOURNAL ARTICLE

Gill MJ, Simjee S, Al-Hattawi K, Robertson BD, Easmon CSF, Ison CAet al., 1998, Gonococcal resistance to beta-lactams and tetracycline involves mutation in loop 3 of the porin encoded at the penB locus, ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, Vol: 42, Pages: 2799-2803, ISSN: 0066-4804

JOURNAL ARTICLE

Young DB, Robertson BD, 1998, Commentary - Approaches to combat tuberculosis, CURRENT OPINION IN BIOTECHNOLOGY, Vol: 9, Pages: 650-652, ISSN: 0958-1669

JOURNAL ARTICLE

Delahay RM, Robertson BD, Balthazar JT, Shafer WM, Ison CAet al., 1997, Involvement of the gonococcal MtrE protein in the resistance of Neisseria gonorrhoeae to toxic hydrophobic agents, MICROBIOLOGY-UK, Vol: 143, Pages: 2127-2133, ISSN: 1350-0872

JOURNAL ARTICLE

Goyal M, Shaw RJ, Banerjee DK, Coker RJ, Robertson BD, Young DBet al., 1997, Rapid detection of multidrug-resistant tuberculosis, EUROPEAN RESPIRATORY JOURNAL, Vol: 10, Pages: 1120-1124, ISSN: 0903-1936

JOURNAL ARTICLE

Heyderman RS, Klein NJ, Daramola OA, Hammerschmidt S, Frosch M, Robertson BD, Levin M, Ison CAet al., 1997, Induction of human endothelial tissue factor expression by Neisseria meningitidis: The influence of bacterial killing and adherence to the endothelium, MICROBIAL PATHOGENESIS, Vol: 22, Pages: 265-274, ISSN: 0882-4010

JOURNAL ARTICLE

Petering H, Hammerschmidt S, Frosch M, vanPutten JPM, Ison CA, Robertson BDet al., 1996, Genes associated with the meningococcal capsule complex are also found in Neisseria gonorrhoeae, JOURNAL OF BACTERIOLOGY, Vol: 178, Pages: 3342-3345, ISSN: 0021-9193

JOURNAL ARTICLE

Shaw RJ, Goyal M, Banerjee D, Coker RJ, Robertson BD, Young DBet al., 1996, Rapid detection of MDR-TB in clinical samples, Thorax, Vol: 51, ISSN: 0040-6376

Clinical management of infection with multi-drug resistant M. tuberculosis (MDR-TB) poses a serious problem for clinicians, not least since diagnosis by conventional microbiological techniques may take many weeks or months. This study reports the use of a rapid approach to making the diagnosis in the setting of suspected nosocomial spread of MDR-TB. Sputum and bronchoalvedar lavage (BAL) samples were examined from two immunocompromised patients who were contacts of the index case. Resistance to rifampicin was identified using the PCR based Inno-LipaRifTB kit and confirmed by automated sequencing. Strain typing was performed using the PCR method of spoligotyping. Sputum from one of the suspected patients was found to contain an identical strain of M. tuberculosis to the index case, and M. tuberculosis DNA in the sputum had a point mutation in the rpoB gene identical to that identified in the isolate from the index case, thus indicating rifampicin resistance and likely nosocomial spread. These results were reported to the clinicians within 48 hours of the time of sputum collection. From the second suspected patient, sputum was unavailable and BAL was smear negative. However, the BAL was found to contain M. tuberculosis DNA without any mutations on the rpoB gene, indicating the presence of an unrelated organism with rifampicin sensitivity, and allowing the patient to be discharged from hospital on conventional triple therapy. This methodology appears to offer a rapid aid to diagnosis for the clinician faced with a patient with suspected MDR-TB.

JOURNAL ARTICLE

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