Imperial College London


Faculty of MedicineDepartment of Medicine

Reader in Systems Microbiology



+44 (0)20 7594 3198b.robertson




Ms Sophie Kemp +44 (0)20 7594 3199




3.41Flowers buildingSouth Kensington Campus






BibTex format

author = {Arafah, S and Kicka, S and Trofimov, V and Hagedorn, M and Andreu, N and Wiles, S and Robertson, B and Soldati, T},
doi = {10.1007/978-1-62703-302-2_22},
journal = {Methods Mol Biol},
pages = {403--417},
title = {Setting up and monitoring an infection of Dictyostelium discoideum with mycobacteria.},
url = {},
volume = {983},
year = {2013}

RIS format (EndNote, RefMan)

AB - Mycobacterium marinum is the causative agent of fish and amphibian tuberculosis in the wild. It is a genetically close cousin of Mycobacterium tuberculosis, and thereby the infection process remarkably shares many of the hallmarks of M. tuberculosis infection in human, at both the cellular and organism levels. Therefore, M. marinum is used as a model for the study of mycobacterial infection in various host organisms. Recently, the Dictyostelium-M. marinum system has been shown to be a valuable model that recapitulates the main features of the intracellular fate of M. marinum including phagosome maturation arrest, as well as its particular cell-to-cell dissemination mode. We present here a "starter kit" of detailed methods that allows to establish an infection of Dictyostelium with M. marinum and to monitor quantitatively the intracellular bacterial growth.
AU - Arafah,S
AU - Kicka,S
AU - Trofimov,V
AU - Hagedorn,M
AU - Andreu,N
AU - Wiles,S
AU - Robertson,B
AU - Soldati,T
DO - 10.1007/978-1-62703-302-2_22
EP - 417
PY - 2013///
SP - 403
TI - Setting up and monitoring an infection of Dictyostelium discoideum with mycobacteria.
T2 - Methods Mol Biol
UR -
UR -
VL - 983
ER -