Publications
130 results found
Stewart GR, Robertson BD, Young DB, 2003, Tuberculosis: A problem with persistence, NATURE REVIEWS MICROBIOLOGY, Vol: 1, Pages: 97-105, ISSN: 1740-1526
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- Citations: 281
Harrison OB, Robertson BD, Faust SN, et al., 2002, Analysis of pathogen-host cell interactions in purpura fulminans:: Expression of capsule, type IV pili, and PorA by <i>Neisseria meningitidis</i> in vivo, INFECTION AND IMMUNITY, Vol: 70, Pages: 5193-5201, ISSN: 0019-9567
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- Citations: 46
Young DB, Robertson BD, 2002, Immune intervention in tuberculosis, Immunology of Infectious Diseases, Pages: 439-451, ISBN: 9781555812140
Ho TBL, Robertson BD, Rhee JT, et al., 2001, Are deletions in the PLCD locus associated with variation in infectivity in clinical isolates of Mycobacterium tuberculosis?, THORAX, Vol: 56, Pages: 56-56, ISSN: 0040-6376
Young D, Robertson B, 2001, Genomics: leprosy - a degenerative disease of the genome., Curr Biol, Vol: 11, Pages: R381-R383, ISSN: 0960-9822
Analysis of the genome of the leprosy bacillus uncovers evidence of extensive deletion and inactivation of genes. Secluded in a specialised niche, it has discarded much of its genetic heritage, though retaining just enough to be a major human pathogen.
Young D, Robertson B, 2001, Genomics: leprosy - a degenerative disease of the genome., Current Biology, Vol: 11
Ho TBL, Robertson BD, Taylor GM, et al., 2000, Comparison of Mycobacterium tuberculosis genomes reveals frequent deletions in a 20 kb variable region in clinical isolates, Yeast, Vol: 17, Pages: 272-282, ISSN: 1097-0061
The Mycobacterium tuberculosis complex is associated with a remarkably low level ofstructural gene polymorphism. As part of a search for alternative forms of geneticvariation that may act as a source of biological diversity in M. tuberculosis, we haveidenti®ed a region of the genome that is highly variable amongst a panel of unrelatedclinical isolates. Fifteen of 24 isolates examined contained one or more copies of the M.tuberculosis-speci®c IS6110 insertion element within this 20 kb variable region. In nine ofthe isolates, including the laboratory-passaged strain H37Rv, genomic deletions wereidenti®ed, resulting in loss of between two and 13 genes. In each case, deletions wereassociated with the presence of a copy of the IS6110 element. Absence of ¯anking tri- ortetra-nucleotide repeats identi®ed homologous recombination between adjacent IS6110elements as the most likely mechanism of the deletion events. IS6110 insertion into hotspotswithin the genome of M. tuberculosis provides a mechanism for generation of geneticdiversity involving a high frequency of insertions and deletions.
Dockrell HM, Brahmbhatt S, Robertson BD, et al., 2000, Diagnostic assays for leprosy based on T-cell epitopes, Workshop on Leprosy Research at the New Millennium, Publisher: LEPRA, Pages: S55-S58, ISSN: 0305-7518
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- Citations: 7
Dockrell HM, Brahmbhatt S, Robertson BD, et al., 2000, A postgenomic approach to identification of <i>Mycobacterium leprae</i>-specific peptides as T-cell reagents, INFECTION AND IMMUNITY, Vol: 68, Pages: 5846-5855, ISSN: 0019-9567
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- Citations: 36
Devyatyrova-Johnson M, Rees IH, Robertson BD, et al., 2000, The lipopolysaccharide structures of <i>Salmonella enterica</i> serovar Typhimurium and <i>Neisseria gonorrhoeae</i> determine the attachment of human mannose-binding lectin to intact organisms, INFECTION AND IMMUNITY, Vol: 68, Pages: 3894-3899, ISSN: 0019-9567
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- Citations: 76
Herrmann JL, Delahay R, Gallagher A, et al., 2000, Analysis of post-translational modification of mycobacterial proteins using a cassette expression system, FEBS LETTERS, Vol: 473, Pages: 358-362, ISSN: 0014-5793
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- Citations: 48
Ho TBL, Robertson BD, Michael Taylor G, et al., 2000, Comparison of Mycobacterium tuberculosis genomes reveals frequent deletions in a 20 kb variable region in clinical isolates, Yeast, Vol: 17, Pages: 272-282, ISSN: 0749-503X
The Alycobactcrium tuberculosis complex is associated with a remarkably low level of structural gene polymorphism. As part of a search for alternative forms of genetic variation that may act as a source of biological diversity in M. tuberculosis, we have identified a region of the genome that is highly variable amongst a panel of unrelated clinical isolates. Fifteen of 24 isolates examined contained one or more copies of the M. tuberculosis-specific IS6110 insertion element within this 20 kb variable region. In nine of the isolates, including the laboratory-passaged strain H37Rv, gcnomic deletions were identified, resulting in loss of between two and 13 genes. In each case, deletions were associated with the presence of a copy of the IS6110 element. Absence of flanking tri- or tetra-nuclcotidc repeats identified homologous recombination between adjacent IS6110 elements as the most likely mechanism of the deletion events. IS61I10 insertion into hotspots within the genome of M. tuberculosis provides a mechanism for generation of genetic diversity involving a high frequency of insertions and deletions. Copyright ©2000 John Wiley & Sons, Ltd.
De Smet KAL, Weston A, Brown IN, et al., 2000, Three pathways for trehalose biosynthesis in mycobacteria, MICROBIOLOGY-UK, Vol: 146, Pages: 199-208, ISSN: 1350-0872
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- Citations: 198
Young DB, Robertson BD, 1999, Microbiology - TB vaccines: Global solutions for global problems, SCIENCE, Vol: 284, Pages: 1479-1480, ISSN: 0036-8075
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- Citations: 12
Young DB, Robertson BD, 1999, TB vaccines: global solutions for global problems., Science, Vol: 284, Pages: 1479-1480, ISSN: 0036-8075
Young DB, Robertson BD, 1998, Commentary - Approaches to combat tuberculosis, CURRENT OPINION IN BIOTECHNOLOGY, Vol: 9, Pages: 650-652, ISSN: 0958-1669
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- Citations: 2
Gill MJ, Simjee S, Al-Hattawi K, et al., 1998, Gonococcal resistance to β-lactams and tetracycline involves mutation in loop 3 of the porin encoded at the <i>penB</i> locus, ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, Vol: 42, Pages: 2799-2803, ISSN: 0066-4804
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- Citations: 114
Delahay RM, Robertson BD, Balthazar JT, et al., 1997, Involvement of the gonococcal MtrE protein in the resistance of Neisseria gonorrhoeae to toxic hydrophobic agents, MICROBIOLOGY-SGM, Vol: 143, Pages: 2127-2133, ISSN: 1350-0872
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- Citations: 72
Heyderman RS, Klein NJ, Daramola OA, et al., 1997, Induction of human endothelial tissue factor expression by Neisseria meningitidis: The influence of bacterial killing and adherence to the endothelium, MICROBIAL PATHOGENESIS, Vol: 22, Pages: 265-274, ISSN: 0882-4010
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- Citations: 31
Goyal M, Shaw RJ, Banerjee DK, et al., 1997, Rapid detection of multidrug-resistant tuberculosis, EUROPEAN RESPIRATORY JOURNAL, Vol: 10, Pages: 1120-1124, ISSN: 0903-1936
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- Citations: 27
Shaw RJ, Goyal M, Banerjee D, et al., 1996, Rapid detection of MDR-TB in clinical samples, Thorax, Vol: 51, ISSN: 0040-6376
Clinical management of infection with multi-drug resistant M. tuberculosis (MDR-TB) poses a serious problem for clinicians, not least since diagnosis by conventional microbiological techniques may take many weeks or months. This study reports the use of a rapid approach to making the diagnosis in the setting of suspected nosocomial spread of MDR-TB. Sputum and bronchoalvedar lavage (BAL) samples were examined from two immunocompromised patients who were contacts of the index case. Resistance to rifampicin was identified using the PCR based Inno-LipaRifTB kit and confirmed by automated sequencing. Strain typing was performed using the PCR method of spoligotyping. Sputum from one of the suspected patients was found to contain an identical strain of M. tuberculosis to the index case, and M. tuberculosis DNA in the sputum had a point mutation in the rpoB gene identical to that identified in the isolate from the index case, thus indicating rifampicin resistance and likely nosocomial spread. These results were reported to the clinicians within 48 hours of the time of sputum collection. From the second suspected patient, sputum was unavailable and BAL was smear negative. However, the BAL was found to contain M. tuberculosis DNA without any mutations on the rpoB gene, indicating the presence of an unrelated organism with rifampicin sensitivity, and allowing the patient to be discharged from hospital on conventional triple therapy. This methodology appears to offer a rapid aid to diagnosis for the clinician faced with a patient with suspected MDR-TB.
Petering H, Hammerschmidt S, Frosch M, et al., 1996, Genes associated with the meningococcal capsule complex are also found in Neisseria gonorrhoeae, JOURNAL OF BACTERIOLOGY, Vol: 178, Pages: 3342-3345, ISSN: 0021-9193
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- Citations: 23
GEMS D, FERGUSON CJ, ROBERTSON BD, et al., 1995, AN ABUNDANT, TRANS-SPLICED MESSENGER-RNA FROM TOXOCARA-CANIS INFECTIVE LARVAE ENCODES A 26-KDA PROTEIN WITH HOMOLOGY TO PHOSPHATIDYLETHANOLAMINE-BINDING PROTEINS, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 270, Pages: 18517-18522, ISSN: 0021-9258
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- Citations: 83
VANPUTTEN JPM, ROBERTSON BD, 1995, MOLECULAR MECHANISMS AND IMPLICATIONS FOR INFECTION OF LIPOPOLYSACCHARIDE VARIATION IN NEISSERIA, MOLECULAR MICROBIOLOGY, Vol: 16, Pages: 847-853, ISSN: 0950-382X
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- Citations: 52
SCHWAN ET, ROBERTSON BD, BRADE H, et al., 1995, GONOCOCCAL RFAF MUTANTS EXPRESS RD(2) CHEMOTYPE LPS AND DO NOT ENTER EPITHELIAL HOST-CELLS, MOLECULAR MICROBIOLOGY, Vol: 15, Pages: 267-275, ISSN: 0950-382X
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- Citations: 37
van Putten JP, Grassmé HU, Robertson BD, et al., 1995, Function of lipopolysaccharide in the invasion of Neisseria gonorrhoeae into human mucosal cells., Prog Clin Biol Res, Vol: 392, Pages: 49-58, ISSN: 0361-7742
The variable incorporation of sialic acid into the LPS of Neisseria gonorrhoeae modulates the invasive behavior of this bacterium towards cultured human epithelial cells. Here we report that the inhibitory effect of LPS sialylation on the gonococcal entry into Chang epithelial cells and ME-180 endocervical cells (van Putten, EMBO J 12:4043-4051, 1993) is located early in the uptake pathway upstream of the invasion-associated recruitment of F-actin at the sites of bacterial entry, but beyond the level of bacterial adherence. Receptor equilibrium studies using purified radiolabelled opacity protein receptor demonstrated that LPS sialylation caused a 3-5 fold reduction in binding of the bacterial invasion-promoting opacity outer membrane protein to its receptor. In HEC-1B and PC-3 cells, LPS sialylation did only partially inhibit the bacterial entry process, suggesting the existence of a second uptake mechanism for gonococci in these cell lines. Experiments with non-sialylatable and truncated isogenic LPS variants, and with genetically defined LPS mutants demonstrated that the invasive phenotype of N. gonorrhoeae requires a minimum of an Rc (or Rd1) chemotype of LPS. Variation within the LPS outer core region did not influence the invasive properties of the bacteria as long as there was no attached sialic acid.
ROBERTSON BD, FROSCH M, VANPUTTEN JPM, 1994, THE IDENTIFICATION OF CRYPTIC RHAMNOSE BIOSYNTHESIS GENES IN NEISSERIA-GONORRHOEAE AND THEIR RELATIONSHIP TO LIPOPOLYSACCHARIDE BIOSYNTHESIS, JOURNAL OF BACTERIOLOGY, Vol: 176, Pages: 6915-6920, ISSN: 0021-9193
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- Citations: 24
HAMMERSCHMIDT S, BIRKHOLZ C, ZAHRINGER U, et al., 1994, CONTRIBUTION OF GENES FROM THE CAPSULE GENE-COMPLEX (CPS) TO LIPOOLIGOSACCHARIDE BIOSYNTHESIS AND SERUM RESISTANCE IN NEISSERIA-MENINGITIDIS, MOLECULAR MICROBIOLOGY, Vol: 11, Pages: 885-896, ISSN: 0950-382X
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- Citations: 120
GILLESPIE SH, BIDWELL D, VOLLER A, et al., 1993, DIAGNOSIS OF HUMAN TOXOCARIASIS BY ANTIGEN CAPTURE ENZYME-LINKED-IMMUNOSORBENT-ASSAY, JOURNAL OF CLINICAL PATHOLOGY, Vol: 46, Pages: 551-554, ISSN: 0021-9746
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- Citations: 40
ROBERTSON BD, FROSCH M, VANPUTTEN JPM, 1993, THE ROLE OF GALE IN THE BIOSYNTHESIS AND FUNCTION OF GONOCOCCAL LIPOPOLYSACCHARIDE, MOLECULAR MICROBIOLOGY, Vol: 8, Pages: 891-901, ISSN: 0950-382X
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- Citations: 65
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