Imperial College London

Dr Charlotte Dean

Faculty of MedicineNational Heart & Lung Institute

Senior Lecturer
 
 
 
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Contact

 

+44 (0)20 7594 3174c.dean

 
 
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Location

 

360Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Akram:2019:10.1038/s41467-019-09067-3,
author = {Akram, K and Yates, L and Mongey, R and Rothery, S and Gaboriau, D and Sanderson, J and Hind, M and Griffiths, M and Dean, C},
doi = {10.1038/s41467-019-09067-3},
journal = {Nature Communications},
title = {Live imaging of alveologenesis in precision-cut lung slices reveals dynamic epithelial cell behaviour},
url = {http://dx.doi.org/10.1038/s41467-019-09067-3},
volume = {10},
year = {2019}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - Damageto alveoli, the gas-exchanging region of the lungs, is a component of many chronic and acute lung diseases. In addition, insufficient generation of alveoli results in bronchopulmonary dysplasia, a disease of prematurity. Therefore visualising the process of alveolar development(alveologenesis) is criticalfor our understanding of lung homeostasis and for the development of treatments to repair and regenerate lung tissue. Using long-term, time-lapse imaging of precision-cut lung slices, we show alveologenesisfor the first time. We reveal that during this process, epithelial cells are highly mobile and we identify specific cell behaviours that contribute to alveologenesis: cell clustering, hollowing and cell extension. Using the cytoskeleton inhibitors blebbistatin and cytochalasin D, weshowed that cell migration is a key driver of alveologenesis. This study reveals important novel information about lung biology and provides a new system inwhich tomanipulate alveologenesis geneticallyandpharmacologically.
AU - Akram,K
AU - Yates,L
AU - Mongey,R
AU - Rothery,S
AU - Gaboriau,D
AU - Sanderson,J
AU - Hind,M
AU - Griffiths,M
AU - Dean,C
DO - 10.1038/s41467-019-09067-3
PY - 2019///
SN - 2041-1723
TI - Live imaging of alveologenesis in precision-cut lung slices reveals dynamic epithelial cell behaviour
T2 - Nature Communications
UR - http://dx.doi.org/10.1038/s41467-019-09067-3
UR - http://hdl.handle.net/10044/1/67093
VL - 10
ER -