Imperial College London
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Dr Charlotte Dean

Faculty of Medicine, National Heart & Lung Institute

Reader in Lung Development and Disease
 
 
 
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Contact

 

+44 (0)20 7594 3174c.dean

 
 
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Location

 

110Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Kim:2021:10.1016/j.biomaterials.2020.120480,
author = {Kim, SY and Mongey, S and Wang, P and Rothery, S and Gabiriau, D and Hind, M and Griffiths, M and Dean, C},
doi = {10.1016/j.biomaterials.2020.120480},
journal = {Biomaterials},
title = {The Acid Injury and Repair (AIR) model: A new ex vivo tool to understand lung repair},
url = {http://dx.doi.org/10.1016/j.biomaterials.2020.120480},
volume = {267},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Research into mechanisms underlying lung injury and subsequent repair responses is currently of paramount importance. There is a paucity of models that bridge the gap between in vitro and in vivo research. Such intermediate models are critical for researchers to decipher the mechanisms that drive repair and to test potential new treatments for lung repair and regeneration. Here we report the establishment of a new tool, the Acid Injury and Repair (AIR) model, that will facilitate studies of lung tissue repair. In this model, injury is applied to a restricted area of a precision-cut lung slice using hydrochloric acid, a clinically relevant driver. The surrounding area remains uninjured, thus mimicking the heterogeneous pattern of injury frequently observed in lung diseases. We show that in response to injury, the percentage of progenitor cells (pro surfactant protein C, proSP-C and TM4SF1 positive) significantly increases in the injured region. Whereas in the uninjured area, the percentage of proSP-C/TM4SF1 cells remains unchanged but proliferating cells (Ki67 positive) increase. These effects are modified in the presence of inhibitors of proliferation (Cytochalasin D) and Wnt secretion (C59) demonstrating that the AIR model is an important new tool for research into lung disease pathogenesis and potential regenerative medicine strategies.
AU  - Kim,SY
AU  - Mongey,S
AU  - Wang,P
AU  - Rothery,S
AU  - Gabiriau,D
AU  - Hind,M
AU  - Griffiths,M
AU  - Dean,C
DO  - 10.1016/j.biomaterials.2020.120480
PY  - 2021///
SN  - 0142-9612
TI  - The Acid Injury and Repair (AIR) model: A new ex vivo tool to understand lung repair
T2  - Biomaterials
UR  - http://dx.doi.org/10.1016/j.biomaterials.2020.120480
UR  - http://hdl.handle.net/10044/1/84868
VL  - 267
ER  -
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