Imperial College London

DrClaireHiggins

Faculty of EngineeringDepartment of Bioengineering

Lecturer
 
 
 
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Contact

 

c.higgins Website

 
 
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Location

 

RSM 3.09Royal School of MinesSouth Kensington Campus

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Summary

 

Publications

Publication Type
Year
to

24 results found

Logan N, Arora H, Higgins C, Evaluating primary blast effects in vitro, Jove-Journal of Visualized Experiments, ISSN: 1940-087X

Understanding how cells are modulated by exposure to shock waves can help identify the mechanisms behind injuries triggered from blast events. This protocol uses custom-built shock tube equipment to apply shock waves at a range of pressures to cell monolayers and to identify the subsequent effects on cell viability.

JOURNAL ARTICLE

Higgins CA, Roger MF, Hill RP, Ali-Khan AS, Garlick JA, Christiano AM, Jahoda CABet al., 2017, Multifaceted role of hair follicle dermal cells in bioengineered skins, BRITISH JOURNAL OF DERMATOLOGY, Vol: 176, Pages: 1259-1269, ISSN: 0007-0963

JOURNAL ARTICLE

Kiani MT, Higgins CA, Almquist BD, 2017, The Hair Follicle: An Underutilized Source of Cells and Materials for Regenerative Medicine, ACS Biomaterials Science & Engineering, ISSN: 2373-9878

JOURNAL ARTICLE

Topouzi H, Logan NJ, Williams G, Higgins CAet al., 2017, Methods for the isolation and 3D culture of dermal papilla cells from human hair follicles., Exp Dermatol

The dermal papilla is a cluster of mesenchymal cells located at the base of the hair follicle which have a number of important roles in the regulation of hair growth. As a consequence, in vitro models of these cells are widely used to study the molecular mechanisms which underlie hair follicle induction, growth and maintenance. While dermal papilla from rodent hair follicles can be digested prior to cell isolation, the unique extracellular matrix composition found in human dermal papilla renders enzymes such as trypsin and collagenase insufficient for digestion of the dermal papilla into a single cell suspension. As such, to grow human dermal papilla cells in vitro, the papilla has to first be isolated via a micro-dissection approach from the follicle. In this paper, we describe the micro-dissection and culture methods which we use within our laboratory, for the study of human dermal papilla cells. This article is protected by copyright. All rights reserved.

JOURNAL ARTICLE

Topouzi H, Higgins CA, 2016, Changing faces: can a new identity stop balding?, EXPERIMENTAL DERMATOLOGY, Vol: 25, Pages: 765-766, ISSN: 0906-6705

JOURNAL ARTICLE

Gledhill K, Guo Z, Umegaki-Arao N, Higgins CA, Itoh M, Christiano AMet al., 2015, Melanin Transfer in Human 3D Skin Equivalents Generated Exclusively from Induced Pluripotent Stem Cells, PLOS ONE, Vol: 10, ISSN: 1932-6203

JOURNAL ARTICLE

Harel S, Higgins CA, Cerise JE, Dai Z, Chen JC, Clynes R, Christiano AMet al., 2015, Pharmacologic inhibition of JAK-STAT signaling promotes hair growth., Sci Adv, Vol: 1

Several forms of hair loss in humans are characterized by the inability of hair follicles to enter the growth phase (anagen) of the hair cycle after being arrested in the resting phase (telogen). Current pharmacologic therapies have been largely unsuccessful in targeting pathways that can be selectively modulated to induce entry into anagen. We show that topical treatment of mouse and human skin with small-molecule inhibitors of the Janus kinase (JAK)-signal transducer and activator of transcription (STAT) pathway results in rapid onset of anagen and subsequent hair growth. We show that JAK inhibition regulates the activation of key hair follicle populations such as the hair germ and improves the inductivity of cultured human dermal papilla cells by controlling a molecular signature enriched in intact, fully inductive dermal papillae. Our findings open new avenues for exploration of JAK-STAT inhibition for promotion of hair growth and highlight the role of this pathway in regulating the activation of hair follicle stem cells.

JOURNAL ARTICLE

Topouzi H, Higgins CA, 2015, Expression map of three distinct skin fibroblast populations isolated from human skin, 45th Annual Meeting of the European-Society-for-Dermatological-Research, Publisher: NATURE PUBLISHING GROUP, Pages: S68-S68, ISSN: 0022-202X

CONFERENCE PAPER

Eungdamrong NJ, Higgins C, Guo Z, Lee W-H, Gillette B, Sia S, Christiano AMet al., 2014, Challenges and promises in modeling dermatologic disorders with bioengineered skin, Experimental Biology and Medicine, Vol: 239, Pages: 1215-1224, ISSN: 1535-3702

JOURNAL ARTICLE

Furniss M, Higgins CA, Martinez-Mir A, Horev L, Petukhova L, Stanimirovic A, Miljkovic J, Zlotogorski A, Christiano AMet al., 2014, Identification of Distinct Mutations in AAGAB in Families with Type 1 Punctate Palmoplantar Keratoderma, JOURNAL OF INVESTIGATIVE DERMATOLOGY, Vol: 134, Pages: 1749-1752, ISSN: 0022-202X

JOURNAL ARTICLE

Higgins CA, 2014, Interrogating the integument: the role of the epidermis in hair induction, EXPERIMENTAL DERMATOLOGY, Vol: 23, Pages: 714-715, ISSN: 0906-6705

JOURNAL ARTICLE

Higgins CA, Christiano AM, 2014, Regenerative medicine and hair loss: how hair follicle culture has advanced our understanding of treatment options for androgenetic alopecia, REGENERATIVE MEDICINE, Vol: 9, Pages: 101-111, ISSN: 1746-0751

JOURNAL ARTICLE

Higgins CA, Petukhova L, Harel S, Ho YY, Drill E, Shapiro L, Wajid M, Christiano AMet al., 2014, FGF5 is a crucial regulator of hair length in humans, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 111, Pages: 10648-10653, ISSN: 0027-8424

JOURNAL ARTICLE

Xing L, Dai Z, Jabbari A, Cerise JE, Higgins CA, Gong W, de Jong A, Harel S, DeStefano GM, Rothman L, Singh P, Petukhova L, Mackay-Wiggan J, Christiano AM, Clynes Ret al., 2014, Alopecia areata is driven by cytotoxic T lymphocytes and is reversed by JAK inhibition, NATURE MEDICINE, Vol: 20, Pages: 1043-1049, ISSN: 1078-8956

JOURNAL ARTICLE

Guo Z, Higgins CA, Gillette BM, Itoh M, Umegaki N, Gledhill K, Sia SK, Christiano AMet al., 2013, Building a microphysiological skin model from induced pluripotent stem cells, STEM CELL RESEARCH & THERAPY, Vol: 4, ISSN: 1757-6512

JOURNAL ARTICLE

Higgins CA, Chen JC, Cerise JE, Jahoda CAB, Christiano AMet al., 2013, Microenvironmental reprogramming by three-dimensional culture enables dermal papilla cells to induce de novo human hair-follicle growth, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 110, Pages: 19679-19688, ISSN: 0027-8424

JOURNAL ARTICLE

Itoh M, Umegaki-Arao N, Guo Z, Liu L, Higgins CA, Christiano AMet al., 2013, Generation of 3D Skin Equivalents Fully Reconstituted from Human Induced Pluripotent Stem Cells (iPSCs), PLOS ONE, Vol: 8, ISSN: 1932-6203

JOURNAL ARTICLE

De Berker D, Higgins CA, Jahoda C, Christiano AMet al., 2012, Biology of Hair and Nails, Dermatology, 3rd Edition, Editors: Bolognia, Jorizzo, Schaffer, Publisher: Elsevier Publishing, ISBN: 9780723435716

Better comprehend the clinical-pathological relationship of skin disease with increased histologic coverage. Bolognia's Dermatology is the ultimate multimedia reference for residents in training AND the experienced practitioner.

BOOK CHAPTER

Higgins CA, Itoh M, Inoue K, Richardson GD, Jahoda CAB, Christiano AMet al., 2012, Reprogramming of Human Hair Follicle Dermal Papilla Cells into Induced Pluripotent Stem Cells, JOURNAL OF INVESTIGATIVE DERMATOLOGY, Vol: 132, Pages: 1725-1727, ISSN: 0022-202X

JOURNAL ARTICLE

Hill RP, Gledhill K, Gardner A, Higgins CA, Crawford H, Lawrence C, Hutchison CJ, Owens WA, Kara B, James SE, Jahoda CABet al., 2012, Generation and Characterization of Multipotent Stem Cells from Established Dermal Cultures, PLOS ONE, Vol: 7, ISSN: 1932-6203

JOURNAL ARTICLE

Higgins CA, Westgate GE, Jahoda CAB, 2011, Modulation in Proteolytic Activity Is Identified as a Hallmark of Exogen by Transcriptional Profiling of Hair Follicles, JOURNAL OF INVESTIGATIVE DERMATOLOGY, Vol: 131, Pages: 2349-2357, ISSN: 0022-202X

JOURNAL ARTICLE

Higgins CA, Richardson GD, Ferdinando D, Westgate GE, Jahoda CABet al., 2010, Modelling the hair follicle dermal papilla using spheroid cell cultures, EXPERIMENTAL DERMATOLOGY, Vol: 19, Pages: 546-548, ISSN: 0906-6705

JOURNAL ARTICLE

Higgins CA, Richardson GD, Westgate GE, Jahoda CABet al., 2009, Exogen involves gradual release of the hair club fibre in the vibrissa follicle model, EXPERIMENTAL DERMATOLOGY, Vol: 18, Pages: 793-795, ISSN: 0906-6705

JOURNAL ARTICLE

Higgins CA, Westgate GE, Jahoda CAB, 2009, From Telogen to Exogen: Mechanisms Underlying Formation and Subsequent Loss of the Hair Club Fiber, JOURNAL OF INVESTIGATIVE DERMATOLOGY, Vol: 129, Pages: 2100-2108, ISSN: 0022-202X

JOURNAL ARTICLE

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