24 results found
Logan N, Arora H, Higgins C, Evaluating primary blast effects in vitro, Jove-Journal of Visualized Experiments, ISSN: 1940-087X
Understanding how cells are modulated by exposure to shock waves can help identify the mechanisms behind injuries triggered from blast events. This protocol uses custom-built shock tube equipment to apply shock waves at a range of pressures to cell monolayers and to identify the subsequent effects on cell viability.
Higgins CA, Roger MF, Hill RP, et al., 2017, Multifaceted role of hair follicle dermal cells in bioengineered skins, BRITISH JOURNAL OF DERMATOLOGY, Vol: 176, Pages: 1259-1269, ISSN: 0007-0963
Kiani MT, Higgins CA, Almquist BD, 2017, The Hair Follicle: An Underutilized Source of Cells and Materials for Regenerative Medicine, ACS Biomaterials Science & Engineering, ISSN: 2373-9878
Topouzi H, Logan NJ, Williams G, et al., 2017, Methods for the isolation and 3D culture of dermal papilla cells from human hair follicles., Exp Dermatol
The dermal papilla is a cluster of mesenchymal cells located at the base of the hair follicle which have a number of important roles in the regulation of hair growth. As a consequence, in vitro models of these cells are widely used to study the molecular mechanisms which underlie hair follicle induction, growth and maintenance. While dermal papilla from rodent hair follicles can be digested prior to cell isolation, the unique extracellular matrix composition found in human dermal papilla renders enzymes such as trypsin and collagenase insufficient for digestion of the dermal papilla into a single cell suspension. As such, to grow human dermal papilla cells in vitro, the papilla has to first be isolated via a micro-dissection approach from the follicle. In this paper, we describe the micro-dissection and culture methods which we use within our laboratory, for the study of human dermal papilla cells. This article is protected by copyright. All rights reserved.
Topouzi H, Higgins CA, 2016, Changing faces: can a new identity stop balding?, EXPERIMENTAL DERMATOLOGY, Vol: 25, Pages: 765-766, ISSN: 0906-6705
Gledhill K, Guo Z, Umegaki-Arao N, et al., 2015, Melanin Transfer in Human 3D Skin Equivalents Generated Exclusively from Induced Pluripotent Stem Cells, PLOS ONE, Vol: 10, ISSN: 1932-6203
Harel S, Higgins CA, Cerise JE, et al., 2015, Pharmacologic inhibition of JAK-STAT signaling promotes hair growth., Sci Adv, Vol: 1
Several forms of hair loss in humans are characterized by the inability of hair follicles to enter the growth phase (anagen) of the hair cycle after being arrested in the resting phase (telogen). Current pharmacologic therapies have been largely unsuccessful in targeting pathways that can be selectively modulated to induce entry into anagen. We show that topical treatment of mouse and human skin with small-molecule inhibitors of the Janus kinase (JAK)-signal transducer and activator of transcription (STAT) pathway results in rapid onset of anagen and subsequent hair growth. We show that JAK inhibition regulates the activation of key hair follicle populations such as the hair germ and improves the inductivity of cultured human dermal papilla cells by controlling a molecular signature enriched in intact, fully inductive dermal papillae. Our findings open new avenues for exploration of JAK-STAT inhibition for promotion of hair growth and highlight the role of this pathway in regulating the activation of hair follicle stem cells.
Topouzi H, Higgins CA, 2015, Expression map of three distinct skin fibroblast populations isolated from human skin, 45th Annual Meeting of the European-Society-for-Dermatological-Research, Publisher: NATURE PUBLISHING GROUP, Pages: S68-S68, ISSN: 0022-202X
Eungdamrong NJ, Higgins C, Guo Z, et al., 2014, Challenges and promises in modeling dermatologic disorders with bioengineered skin, Experimental Biology and Medicine, Vol: 239, Pages: 1215-1224, ISSN: 1535-3702
Furniss M, Higgins CA, Martinez-Mir A, et al., 2014, Identification of Distinct Mutations in AAGAB in Families with Type 1 Punctate Palmoplantar Keratoderma, JOURNAL OF INVESTIGATIVE DERMATOLOGY, Vol: 134, Pages: 1749-1752, ISSN: 0022-202X
Higgins CA, 2014, Interrogating the integument: the role of the epidermis in hair induction, EXPERIMENTAL DERMATOLOGY, Vol: 23, Pages: 714-715, ISSN: 0906-6705
Higgins CA, Christiano AM, 2014, Regenerative medicine and hair loss: how hair follicle culture has advanced our understanding of treatment options for androgenetic alopecia, REGENERATIVE MEDICINE, Vol: 9, Pages: 101-111, ISSN: 1746-0751
Higgins CA, Petukhova L, Harel S, et al., 2014, FGF5 is a crucial regulator of hair length in humans, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 111, Pages: 10648-10653, ISSN: 0027-8424
Xing L, Dai Z, Jabbari A, et al., 2014, Alopecia areata is driven by cytotoxic T lymphocytes and is reversed by JAK inhibition, NATURE MEDICINE, Vol: 20, Pages: 1043-1049, ISSN: 1078-8956
Guo Z, Higgins CA, Gillette BM, et al., 2013, Building a microphysiological skin model from induced pluripotent stem cells, STEM CELL RESEARCH & THERAPY, Vol: 4, ISSN: 1757-6512
Higgins CA, Chen JC, Cerise JE, et al., 2013, Microenvironmental reprogramming by three-dimensional culture enables dermal papilla cells to induce de novo human hair-follicle growth, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 110, Pages: 19679-19688, ISSN: 0027-8424
Itoh M, Umegaki-Arao N, Guo Z, et al., 2013, Generation of 3D Skin Equivalents Fully Reconstituted from Human Induced Pluripotent Stem Cells (iPSCs), PLOS ONE, Vol: 8, ISSN: 1932-6203
De Berker D, Higgins CA, Jahoda C, et al., 2012, Biology of Hair and Nails, Dermatology, 3rd Edition, Editors: Bolognia, Jorizzo, Schaffer, Publisher: Elsevier Publishing, ISBN: 9780723435716
Better comprehend the clinical-pathological relationship of skin disease with increased histologic coverage. Bolognia's Dermatology is the ultimate multimedia reference for residents in training AND the experienced practitioner.
Higgins CA, Itoh M, Inoue K, et al., 2012, Reprogramming of Human Hair Follicle Dermal Papilla Cells into Induced Pluripotent Stem Cells, JOURNAL OF INVESTIGATIVE DERMATOLOGY, Vol: 132, Pages: 1725-1727, ISSN: 0022-202X
Hill RP, Gledhill K, Gardner A, et al., 2012, Generation and Characterization of Multipotent Stem Cells from Established Dermal Cultures, PLOS ONE, Vol: 7, ISSN: 1932-6203
Higgins CA, Westgate GE, Jahoda CAB, 2011, Modulation in Proteolytic Activity Is Identified as a Hallmark of Exogen by Transcriptional Profiling of Hair Follicles, JOURNAL OF INVESTIGATIVE DERMATOLOGY, Vol: 131, Pages: 2349-2357, ISSN: 0022-202X
Higgins CA, Richardson GD, Ferdinando D, et al., 2010, Modelling the hair follicle dermal papilla using spheroid cell cultures, EXPERIMENTAL DERMATOLOGY, Vol: 19, Pages: 546-548, ISSN: 0906-6705
Higgins CA, Richardson GD, Westgate GE, et al., 2009, Exogen involves gradual release of the hair club fibre in the vibrissa follicle model, EXPERIMENTAL DERMATOLOGY, Vol: 18, Pages: 793-795, ISSN: 0906-6705
Higgins CA, Westgate GE, Jahoda CAB, 2009, From Telogen to Exogen: Mechanisms Underlying Formation and Subsequent Loss of the Hair Club Fiber, JOURNAL OF INVESTIGATIVE DERMATOLOGY, Vol: 129, Pages: 2100-2108, ISSN: 0022-202X
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