Imperial College London
Alternate

DrClaireHiggins

Faculty of Engineering, Department of Bioengineering

Reader in Tissue Regeneration
 
 
 
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Contact

 

c.higgins Website

 
 
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Location

 

Uren 319Sir Michael Uren HubWhite City Campus

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Summary

 

Publications

Citation

BibTex format

@article{Al:2019:10.1021/acsnano.9b04783,
author = {Al, Sulaiman D and Chang, JYH and Bennett, NR and Topouzi, H and Higgins, CA and Irvine, DJ and Ladame, S},
doi = {10.1021/acsnano.9b04783},
journal = {ACS Nano},
pages = {9620--9628},
title = {Hydrogel-coated microneedle arrays for minimally invasive sampling and sensing of specific circulating nucleic acids from skin interstitial fluid},
url = {http://dx.doi.org/10.1021/acsnano.9b04783},
volume = {13},
year = {2019}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Minimally invasive technologies that can sample and detect cell-free nucleic acid biomarkers from liquid biopsies have recently emerged as clinically useful for early diagnosis of a broad range of pathologies, including cancer. Although blood has so far been the most commonly interrogated bodily fluid, skin interstitial fluid has been mostly overlooked despite containing the same broad variety of molecular biomarkers originating from cells and surrounding blood capillaries. Emerging technologies to sample this fluid in a pain-free and minimally-invasive manner often take the form of microneedle patches. Herein, we developed microneedles that are coated with an alginate–peptide nucleic acid hybrid material for sequence-specific sampling, isolation, and detection of nucleic acid biomarkers from skin interstitial fluid. Characterized by fast sampling kinetics and large sampling capacity (∼6.5 μL in 2 min), this platform technology also enables the detection of specific nucleic acid biomarkers either on the patch itself or in solution after light-triggered release from the hydrogel. Considering the emergence of cell-free nucleic acids in bodily fluids as clinically informative biomarkers, platform technologies that can detect them in an automated and minimally invasive fashion have great potential for personalized diagnosis and longitudinal monitoring of patient-specific disease progression.
AU  - Al,Sulaiman D
AU  - Chang,JYH
AU  - Bennett,NR
AU  - Topouzi,H
AU  - Higgins,CA
AU  - Irvine,DJ
AU  - Ladame,S
DO  - 10.1021/acsnano.9b04783
EP  - 9628
PY  - 2019///
SN  - 1936-0851
SP  - 9620
TI  - Hydrogel-coated microneedle arrays for minimally invasive sampling and sensing of specific circulating nucleic acids from skin interstitial fluid
T2  - ACS Nano
UR  - http://dx.doi.org/10.1021/acsnano.9b04783
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000484077800113&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://pubs.acs.org/doi/10.1021/acsnano.9b04783
UR  - http://hdl.handle.net/10044/1/74646
VL  - 13
ER  -
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