Imperial College London
Alternate

ProfessorClareLloyd

Faculty of Medicine, National Heart & Lung Institute

Interim Head of NHLI, Vice-Dean (institutional Affairs) FoM
 
 
 
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Contact

 

+44 (0)20 7594 3102c.lloyd Website

 
 
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Location

 

Office 352Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Allden:2019:10.1164/rccm.201809-1775OC,
author = {Allden, SJ and Ogger, PP and Ghai, P and McErlean, P and Hewitt, R and Toshner, R and Walker, SA and Saunders, P and Kingston, S and Molyneaux, PL and Maher, TM and Lloyd, CM and Byrne, AJ},
doi = {10.1164/rccm.201809-1775OC},
journal = {American Journal of Respiratory and Critical Care Medicine},
title = {The transferrin receptor CD71 delineates functionally distinct airway macrophage subsets during idiopathic pulmonary fibrosis},
url = {http://dx.doi.org/10.1164/rccm.201809-1775OC},
volume = {200},
year = {2019}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - RATIONALE: Idiopathic pulmonary fibrosis (IPF) is a devastating progressive disease with limited therapeutic options. Airway macrophages (AMs) are key components of the defence of the airways and are implicated in the pathogenesis of IPF. Alterations in iron metabolism have been described during fibrotic lung disease and in murine models of lung fibrosis. However, the role of transferrin receptor-1 (CD71)-expressing AMs in IPF is not known. OBJECTIVES: To assess the role of CD71 expressing AMs in the IPF-lung. METHODS: We utilized multi-parameter flow cytometry, gene expression analysis and phagocytosis/transferrin uptake assays to delineate the role of AMs expressing, or lacking, CD71 in the BAL of patients with IPF or healthy controls. MEASUREMENTS AND MAIN RESULTS: There was a distinct increase in proportions of AMs lacking CD71 in IPF patients in comparison to healthy controls. Levels of BAL transferrin were enhanced in IPF-BAL and furthermore, CD71- AMs had an impaired ability to sequester transferrin. CD71+ and CD71- AMs were phenotypically, functionally and transcriptionally distinct, with CD71- AMs characterised by reduced expression of markers of macrophage maturity, impaired phagocytosis and enhanced expression of pro-fibrotic genes. Importantly, proportions of AMs lacking CD71 were independently associated with worse survival, underlining the importance of this population in IPF and as a potential therapeutic target. CONCLUSIONS: Taken together these data highlight how CD71 delineates AM subsets which play distinct roles in IPF and furthermore, CD71- AMs may be an important pathogenic component of fibrotic lung disease.
AU  - Allden,SJ
AU  - Ogger,PP
AU  - Ghai,P
AU  - McErlean,P
AU  - Hewitt,R
AU  - Toshner,R
AU  - Walker,SA
AU  - Saunders,P
AU  - Kingston,S
AU  - Molyneaux,PL
AU  - Maher,TM
AU  - Lloyd,CM
AU  - Byrne,AJ
DO  - 10.1164/rccm.201809-1775OC
PY  - 2019///
SN  - 1073-449X
TI  - The transferrin receptor CD71 delineates functionally distinct airway macrophage subsets during idiopathic pulmonary fibrosis
T2  - American Journal of Respiratory and Critical Care Medicine
UR  - http://dx.doi.org/10.1164/rccm.201809-1775OC
UR  - https://www.ncbi.nlm.nih.gov/pubmed/31051082
UR  - https://www.atsjournals.org/doi/10.1164/rccm.201809-1775OC
UR  - http://hdl.handle.net/10044/1/70043
VL  - 200
ER  -
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