For more information on the lab, including details of any open position, visit our group website here.
In vivo imaging of haematopoietic stem and progenitor cells in the bone marrow microenvironment
In vivo image of a fluorescently labelled HSC (white) observed in the calvarium (top of the skull) bone marrow of an irradiated, anesthetised, Col2.3GFP recipient mouse one hour after transplantation.
Three components of the bone marrow microenvironment are visualised. The osteoblasts (bone making cells lining the endosteal surface) express high levels of GFP and are in green. Bone collagen (blue) is detected by two-photon microscopy second harmonic generation. The vasculature (red) is visualised through intravenous injection of non-targeted, circulating quantum dots®. Haematopoietic stem and progenitor cells are labelled with lipophilic membrane dyes prior transplantation. For this image, confocal microscopy was used to detect GFP, quantum dots and the injected cells; two-photon microscopy was used to detect collagen. Scale bar: 50 micrometers.
From time to time there may be open positions to join Dr Lo Celso’s research team. These will be posted on the group website (here: link to http://www3.imperial.ac.uk/locelsolab/group). If there are no open open position but you are interested in joining the group as a postdoc or PhD student, please get in touch directly via email.
Dr Lo Celso teaches and co-organises with Dr Anita Hall the Stem Cells, Regeneration and Ageing (SCRA) course for the 3rd year of the Biochemistry and Biology degrees. She also teaches development of the immune system and haematopoietic stem cell biology in the Immunology courses of Biochemistry and Biology degrees and cell and stem cell biology in the Cell and Developmental Biology course of the Biology degree. These are 2nd year courses.
Dr Lo Celso runs a number of tutorials for first, second and third year courses as well as the SCRA practical. She is personal tutor for the Biochemistry degree, supervises third year literature projects and accommodates third year Biochemistry laboratory projects within her research group.
et al., 2015, Automated Identification and Localization of Hematopoietic Stem Cells in 3D Intravital Microscopy Data, Stem Cell Reports, Vol:5, ISSN:2213-6711, Pages:139-153
et al., 2014, Haematopoietic focal adhesion kinase deficiency alters haematopoietic homeostasis to drive tumour metastasis., Nat Commun, Vol:5
et al., 2014, In vivo time-lapse imaging shows diverse niche engagement by quiescent and naturally activated hematopoietic stem cells, Blood, Vol:124, ISSN:0006-4971, Pages:79-83
Scott MK, Akinduro O, Lo Celso C, 2014, In vivo 4-dimensional tracking of hematopoietic stem and progenitor cells in adult mouse calvarial bone marrow., J Vis Exp
et al., 2013, Deciphering hematopoietic stem cells in their niches: a critical appraisal of genetic models, lineage tracing, and imaging strategies., Cell Stem Cell, Vol:13, Pages:520-533