Imperial College London
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Dr Charis Pericleous

Faculty of Medicine, National Heart & Lung Institute

Advanced Research Fellow
 
 
 
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Contact

 

+44 (0)20 7594 2728c.pericleous Website

 
 
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Location

 

ICTEM buildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{McDonnell:2015:10.1186/s12896-015-0222-0,
author = {McDonnell, T and Pericleous, C and Laurine, E and Tommasi, R and Garza-Garcia, A and Giles, I and Ioannou, Y and Rahman, A},
doi = {10.1186/s12896-015-0222-0},
journal = {BMC Biotechnology},
title = {Development of a high yield expression and purification system for Domain I of Beta-2-glycoprotein I for the treatment of APS},
url = {http://dx.doi.org/10.1186/s12896-015-0222-0},
volume = {15},
year = {2015}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - BackgroundIn this paper we describe a novel method to achieve high yield bacterial expression of a small protein domain with considerable therapeutic potential; Domain I of Beta-2-glycoprotein I (β2GPI). β2GPI is intrinsic to the pathological progression of the Antiphospholipid Syndrome (APS). Patients develop autoantibodies targeting an epitope located on the N-terminal Domain I of β2GPI rendering this domain of interest as a possible therapeutic.ResultsThis new method of production of Domain I of β2GPI has increased the production yield by ~20 fold compared to previous methods in E.coli. This largely scalable, partially automated method produces 50–75 mg of pure, folded, active Domain I of β2GPI per litre of expression media.ConclusionThe application of this method may enable production of Domain I on sufficient scale to allow its use as a therapeutic.
AU  - McDonnell,T
AU  - Pericleous,C
AU  - Laurine,E
AU  - Tommasi,R
AU  - Garza-Garcia,A
AU  - Giles,I
AU  - Ioannou,Y
AU  - Rahman,A
DO  - 10.1186/s12896-015-0222-0
PY  - 2015///
SN  - 1472-6750
TI  - Development of a high yield expression and purification system for Domain I of Beta-2-glycoprotein I for the treatment of APS
T2  - BMC Biotechnology
UR  - http://dx.doi.org/10.1186/s12896-015-0222-0
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000364766600001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/68396
VL  - 15
ER  -
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