Imperial College London
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Dr Charis Pericleous

Faculty of Medicine, National Heart & Lung Institute

Advanced Research Fellow
 
 
 
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Contact

 

+44 (0)20 7594 2728c.pericleous Website

 
 
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Location

 

ICTEM buildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Poulton:2015:10.1111/aji.12341,
author = {Poulton, K and Ripoll, VM and Pericleous, C and Meroni, PL and Gerosa, M and Ioannou, Y and Rahman, A and Giles, IP},
doi = {10.1111/aji.12341},
journal = {American Journal of Reproductive Immunology},
pages = {390--401},
title = {Purified IgG from patients with obstetric but not IgG from non-obstetric antiphospholipid syndrome inhibit trophoblast invasion},
url = {http://dx.doi.org/10.1111/aji.12341},
volume = {73},
year = {2015}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - ProblemSome patients with antiphospholipid syndrome (APS) suffer pregnancy morbidity (PM) but not vascular thrombosis (VT), whilst others suffer VT only. Therefore, we compared the effects of IgG from VT+/PM− and VT−/PM+ subjects on human firsttrimester trophoblast (HTR8) cells.Method of studyHTR8 cells were incubated with APS VT+/PM−, APS VT−/PM+ or healthy control (HC) IgG. We measured trophoblast invasion by cell invasion assay; mRNA expression of TLR4 and adaptor proteins; phosphorylation of p38 MAPK, NFκB and ERK; and expression of interleukin (IL)8 and IL6.ResultsVT−/PM+ IgG, but not VT+/PM− IgG significantly reduced HTR8 invasion. The effects on invasion were blocked by TLR4 inhibition. Neither VT+/PM− nor VT−/PM+ IgG altered MyD88 mRNA expression, phosphorylation of signalling molecules or cytokine expression.ConclusionsVT−/PM+ IgG exert functionally relevant effects on human trophoblast cells but VT+/PM− IgG do not.
AU  - Poulton,K
AU  - Ripoll,VM
AU  - Pericleous,C
AU  - Meroni,PL
AU  - Gerosa,M
AU  - Ioannou,Y
AU  - Rahman,A
AU  - Giles,IP
DO  - 10.1111/aji.12341
EP  - 401
PY  - 2015///
SN  - 1046-7408
SP  - 390
TI  - Purified IgG from patients with obstetric but not IgG from non-obstetric antiphospholipid syndrome inhibit trophoblast invasion
T2  - American Journal of Reproductive Immunology
UR  - http://dx.doi.org/10.1111/aji.12341
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000352810200002&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/68387
VL  - 73
ER  -
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