Publications
239 results found
Shiu KY, McLaughlin L, Rebollo-Mesa I, et al., 2015, B-lymphocytes support and regulate indirect T-cell alloreactivity in individual patients with chronic antibody-mediated rejection, KIDNEY INTERNATIONAL, Vol: 88, Pages: 560-568, ISSN: 0085-2538
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- Citations: 36
Arlt VM, Krais AM, Godschalk RW, et al., 2015, Pulmonary inflammation impacts on CYP1A1-mediated respiratory tract DNA damage induced by the carcinogenic air pollutant benzo[a]pyrene, Toxicological Sciences, Vol: 146, Pages: 213-225, ISSN: 1096-0929
Pulmonary inflammation can contribute to the development of lung cancer in humans. We investigated whether pulmonary inflammation alters the genotoxicity of polycyclic aromatic hydrocarbons (PAHs) in the lungs of mice and what mechanisms are involved. To model nonallergic acute inflammation, mice were exposed intranasally to lipopolysaccharide (LPS; 20 µg/mouse) and then instilled intratracheally with benzo[a]pyrene (BaP; 0.5 mg/mouse). BaP-DNA adduct levels, measured by 32P-postlabeling analysis, were approximately 3-fold higher in the lungs of LPS/BaP-treated mice than in mice treated with BaP alone. Pulmonary Cyp1a1 enzyme activity was decreased in LPS/BaP-treated mice relative to BaP-treated mice suggesting that pulmonary inflammation impacted on BaP-induced Cyp1a1 activity in the lung. Our results showed that Cyp1a1 appears to be important for BaP detoxification in vivo and that the decrease of pulmonary Cyp1a1 activity in LPS/BaP-treated mice results in a decrease of pulmonary BaP detoxification, thereby enhancing BaP genotoxicity (ie, DNA adduct formation) in the lung. Because less BaP was detoxified by Cyp1a1 in the lungs of LPS/BaP-treated mice, more BaP circulated via the blood to extrapulmonary tissues relative to mice treated with BaP only. Indeed, we observed higher BaP-DNA adduct levels in livers of LPS/BaP-treated mice compared with BaP-treated mice. Our results indicate that pulmonary inflammation could be a critical determinant in the induction of genotoxicity in the lung by PAHs like BaP. Cyp1a1 appears to be involved in both BaP bioactivation and detoxification although the contribution of other enzymes to BaP-DNA adduct formation in lung and liver under inflammatory conditions remains to be explored.
de Kort H, Moran L, Roufosse C, 2015, The role of electron microscopy in renal allograft biopsy evaluation, CURRENT OPINION IN ORGAN TRANSPLANTATION, Vol: 20, Pages: 333-342, ISSN: 1087-2418
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- Citations: 9
Koutroutsos K, Charif R, Roufosse C, et al., 2015, Successful Management of Post Transplant Focal Segmental Glomerulosclerosis With Plasma Exchange and Rituximab, American Transplant Congress, Publisher: WILEY-BLACKWELL, ISSN: 1600-6135
Koutroutsos K, Charif R, Goodall D, et al., 2015, Recurrent IgA Nephropathy in Kidney Transplant Recipients Receiving a Steroid Sparing Immunosuppression Protocol, American Transplant Congress, Publisher: WILEY-BLACKWELL, ISSN: 1600-6135
Adam B, Afzali B, Shah N, et al., 2015, Multicenter Validation of a Molecular Diagnostic for Antibody-Mediated Rejection in Formalin-Fixed Paraffin-Embedded Human Renal Allograft Biopsies, American Transplant Congress, Publisher: WILEY-BLACKWELL, ISSN: 1600-6135
McLean A, Dominy K, Sosinsky A, et al., 2015, Use of RNA Sequencing for Discovery of Markers of Accommodation in Surveillance Biopsies from Recipients of an ABO-Incompatible Renal Transplant, American Transplant Congress, Publisher: WILEY-BLACKWELL, ISSN: 1600-6135
Willicombe M, Roufosse C, Galliford J, et al., 2015, Treatment of Subclinical Rejection Identified On Surveillance Biopsy May Prevent the Development of Chronic Alloimmune Injury, American Transplant Congress, Publisher: WILEY-BLACKWELL, ISSN: 1600-6135
Owen-Casey MP, Sim R, Cook HT, et al., 2014, Value of antibodies to free light chains in immunoperoxidase studies of renal biopsies, Journal of Clinical Pathology, Vol: 67, Pages: 661-666, ISSN: 1472-4146
Aims Because immunoglobulin abnormalities may affect the kidney, investigation of renal biopsies requires immunohistological study of light chains. A problem is that most antibodies to light chains react with whole immunoglobulins as well as free light chains, and there are generally many more whole immunoglobulins than free light chains. The usefulness of antibodies that only detected free light chains was investigated.Methods Antibodies to free light chains were used in an immunoperoxidase method on paraffin sections of 198 renal biopsies, and compared with conventional antibodies against light chains examined by immunofluorescence on 13 frozen sections and by immunoperoxidase on 46 paraffin sections.Results Immunofluorescence and immunoperoxidase were concordant on 10 of 13 biopsies. Immunofluorescence detected slight deposition of light chains in three biopsies not shown by immunoperoxidase, of undetermined clinical significance. Using immunoperoxidase, the free light chain antibodies were more sensitive than conventional antibodies, giving much cleaner staining and better detection of deposits in AL amyloid, light chain deposition disease and cryoglobulinaemic glomerulonephritis. The free light chain antibodies showed discordance or ambiguity between immunohistological and clinical findings in seven (4%) of 185 patients with known immunoglobulin status. These included two of 28 cases of AL amyloid that showed no light chain deposition. The method was not designed for detection of light chain restriction in neoplastic plasma or lymphoplasmacytic cells.Conclusions Polyclonal antibodies to free light chains are an improvement on conventional antibodies in immunoperoxidase study of paraffin sections of renal biopsies and are useful in everyday practice.
Roufosse C, Al-Johani T, Willicombe M, et al., 2014, EBV-positive lymphoid infiltrate in a renal surveillance biopsy mimicking subclinical T-cell mediated rejection, VIRCHOWS ARCHIV, Vol: 465, Pages: S265-S265, ISSN: 0945-6317
Clarke C, Moran L, Moss J, et al., 2014, Ultrastructural Features of Chronic Antibody-Mediated Damage in Highly Sensitised Patients With or Without C1q Activating Anti-HLA Antibodies., World Transplant Congress, Publisher: LIPPINCOTT WILLIAMS & WILKINS, Pages: 508-508, ISSN: 0041-1337
Dodd P, Willicombe M, Brookes P, et al., 2014, Significance of HLA IgM and IgM Donor Specific Antibodies in Renal Transplantation., World Transplant Congress, Publisher: LIPPINCOTT WILLIAMS & WILKINS, Pages: 435-435, ISSN: 0041-1337
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- Citations: 3
Koutroutsos K, Willicombe M, Charif R, et al., 2014, Alemtuzumab Induction and Post-Transplant Glomerulonephritis in Kidney Transplantation., World Transplant Congress, Publisher: LIPPINCOTT WILLIAMS & WILKINS, Pages: 534-535, ISSN: 0041-1337
de Kort H, Charif R, Brookes P, et al., 2014, Histological Assessment of 164 ABO Incompatible Renal Transplant Biopsies., World Transplant Congress, Publisher: LIPPINCOTT WILLIAMS & WILKINS, Pages: 562-562, ISSN: 0041-1337
McAdoo S, Bhangal G, Smith J, et al., 2014, Spleen Tyrosine Kinase (SYK) Expression in Antibody-Mediated Renal Allograft Rejection, World Transplant Congress, Publisher: LIPPINCOTT WILLIAMS & WILKINS, Pages: 513-514, ISSN: 0041-1337
Clarke C, Lawrence C, Willicombe M, et al., 2014, IgG Donor Specific Antibodies [DSAs] in Patients With Transplant Glomerulopathy [TG] Are Associated With Inferior Allograft Survival, World Transplant Congress, Publisher: LIPPINCOTT WILLIAMS & WILKINS, Pages: 440-440, ISSN: 0041-1337
McLean A, Brookes P, Charif R, et al., 2014, One Agent-Once a Day: 2 Year Outcomes of a Prospective Randomized Trial of Once Daily (Extended Release) Tacrolimus Maintenance Monotherapy vs Twice Daily (Standard Release) Tacrolimus After Alemtuzumab Induction & Early Steroid Cessation (TAESR Trial). Rejection and Variation in Tacrolimus Levels., World Transplant Congress, Publisher: LIPPINCOTT WILLIAMS & WILKINS, Pages: 536-536, ISSN: 0041-1337
McLean A, Chan K, Roufosse C, et al., 2014, 5-Year Outcomes of a Randomized Prospective Trial of Tacrolimus Maintenance Monotherapy After Alemtuzumab Induction and Early Steroid Withdrawal in Kidney Transplantation: Rejection, HLA Antibody Formation, and Recurrent Disease., World Transplant Congress, Publisher: LIPPINCOTT WILLIAMS & WILKINS, Pages: 114-114, ISSN: 0041-1337
Willicombe M, Roufosse C, Moran L, et al., 2014, Significance of Tubuloreticular Inclusions in Renal Allografts, World Transplant Congress, Publisher: LIPPINCOTT WILLIAMS & WILKINS, Pages: 529-529, ISSN: 0041-1337
de Kort H, Charif R, Brookes P, et al., 2014, Histological Assessment of 164 ABO Incompatible Renal Transplant Biopsies., World Transplant Congress, Publisher: WILEY-BLACKWELL, Pages: 562-562, ISSN: 1600-6135
Clarke C, Moran L, Moss J, et al., 2014, Ultrastructural Features of Chronic Antibody-Mediated Damage in Highly Sensitised Patients With or Without C1q Activating Anti-HLA Antibodies., World Transplant Congress, Publisher: WILEY-BLACKWELL, Pages: 508-508, ISSN: 1600-6135
McLean A, Brookes P, Charif R, et al., 2014, One Agent-Once a Day: 2 Year Outcomes of a Prospective Randomized Trial of Once Daily (Extended Release) Tacrolimus Maintenance Monotherapy vs Twice Daily (Standard Release) Tacrolimus After Alemtuzumab Induction & Early Steroid Cessation (TAESR Trial). Rejection and Variation in Tacrolimus Levels, World Transplant Congress, Publisher: WILEY-BLACKWELL, Pages: 536-536, ISSN: 1600-6135
Clarke C, Lawrence C, Willicombe M, et al., 2014, IgG Donor Specific Antibodies [DSAs] in Patients With Transplant Glomerulopathy [TG] Are Associated With Inferior Allograft Survival, World Transplant Congress, Publisher: WILEY-BLACKWELL, Pages: 440-440, ISSN: 1600-6135
McAdoo S, Bhangal G, Smith J, et al., 2014, Spleen Tyrosine Kinase (SYK) Expression in Antibody-Mediated Renal Allograft Rejection, World Transplant Congress, Publisher: WILEY-BLACKWELL, Pages: 513-514, ISSN: 1600-6135
McLean A, Chan K, Roufosse C, et al., 2014, 5-Year Outcomes of a Randomized Prospective Trial of Tacrolimus Maintenance Monotherapy After Alemtuzumab Induction and Early Steroid Withdrawal in Kidney Transplantation: Rejection, HLA Antibody Formation, and Recurrent Disease., World Transplant Congress, Publisher: WILEY-BLACKWELL, Pages: 114-114, ISSN: 1600-6135
Dodd P, Willicombe M, Brookes P, et al., 2014, Significance of HLA IgM and IgM Donor Specific Antibodies in Renal Transplantation, World Transplant Congress, Publisher: WILEY-BLACKWELL, Pages: 435-435, ISSN: 1600-6135
Willicombe M, Roufosse C, Moran L, et al., 2014, Significance of Tubuloreticular Inclusions in Renal Allografts., World Transplant Congress, Publisher: WILEY-BLACKWELL, Pages: 529-529, ISSN: 1600-6135
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- Citations: 1
Koutroutsos K, Willicombe M, Charif R, et al., 2014, Alemtuzumab Induction and Post-Transplant Glomerulonephritis in Kidney Transplantation, World Transplant Congress, Publisher: WILEY-BLACKWELL, Pages: 534-535, ISSN: 1600-6135
Beckwith H, Medjeral-Thomas N, Galliford J, et al., 2014, Mycophenolate mofetil therapy in IgA nephropathy: histological changes after treatment, SCOTTISH MEDICAL JOURNAL, Vol: 59, Pages: E26-E26, ISSN: 0036-9330
de Kort H, Mallat MJK, van Kooten C, et al., 2014, Diagnosis of Early Pancreas Graft Failure via Antibody-Mediated Rejection: Single-Center Experience With 256 Pancreas Transplantations, AMERICAN JOURNAL OF TRANSPLANTATION, Vol: 14, Pages: 936-942, ISSN: 1600-6135
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- Citations: 15
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