My research focuses on the collection and computational analysis of high-throughput sequencing data with a focus made on Motor Neuron Disease. This follows doctoral and post-doctoral positions within the molecular biology lab of Prof. Matthew Wood (Oxford, UK), functional genomics group of Prof. Jernej Ule (MRC-LMB and UCL, UK) and systems biology team of Dr Mike Inouye (Melbourne, Aus). More specifically, my group uses functional genomics and computational analysis to research master regulators in both post-mortem tissue and human cell models of Motor Neuron Disease. This is with the aim of identifying networks that are perturbed in disease and which contribute to the disease phenotype. Ultimately we plan to use these network models to predict interactions that might be corrected to allow phenotypic recovery.
Separately, and following on from my previous studies, we are exploring disease dysregulation of the numerous un-annotated and non-canonical features of the transcriptome. This is because much remains to be learned about the biological functions, biogenesis mechanisms and the contributions to disease of these fascinating, un-conventional and CNS-enriched transcripts.
et al., 2017, A neuroprotective astrocyte state is induced by neuronal signal EphB1 but fails in ALS models, Nature Communications, Vol:8, ISSN:2041-1723
et al., 2017, Progressive Motor Neuron Pathology and the Role of Astrocytes in a Human Stem Cell Model of VCP-Related ALS, Cell Reports, Vol:19, ISSN:2211-1247, Pages:1739-1749
Sibley CR, Blazquez L, Ule J, 2016, Lessons from non-canonical splicing, Nature Reviews Genetics, Vol:17, ISSN:1471-0056, Pages:407-421
Sibley CR, Wood MJA, 2011, Identification of Allele-Specific RNAi Effectors Targeting Genetic Forms of Parkinson's Disease, PLOS One, Vol:6, ISSN:1932-6203
Modic M, Ule J, Sibley CR, 2013, CLIPing the brain: Studies of protein-RNA interactions important for neurodegenerative disorders, Molecular and Cellular Neuroscience, Vol:56, ISSN:1044-7431, Pages:429-435
et al., 2014, iCLIP: Protein-RNA interactions at nucleotide resolution, Methods, Vol:65, ISSN:1046-2023, Pages:274-287
et al., 2015, Recursive splicing in long vertebrate genes, Nature, Vol:521, ISSN:0028-0836, Pages:371-+