Imperial College London
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ProfessorCesareTerracciano

Faculty of Medicine, National Heart & Lung Institute

Professor of Cardiac Electrophysiology
 
 
 
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Contact

 

+44 (0)20 7594 2735c.terracciano Website CV

 
 
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Location

 

430ICTEM buildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Sanzari:2018:10.1038/s41598-018-30360-6,
author = {Sanzari, I and Humphrey, EJ and Dinelli, F and Terracciano, CM and Prodromakis, T},
doi = {10.1038/s41598-018-30360-6},
journal = {Scientific Reports},
title = {Effect of patterned polyacrylamide hydrogel on morphology and orientation of cultured NRVMs},
url = {http://dx.doi.org/10.1038/s41598-018-30360-6},
volume = {8},
year = {2018}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - We recently demonstrated that patterned Parylene C films could be effectively used as a mask for directly copolymerizing proteins on polyacrylamide hydrogel (PAm). In this work, we have proved the applicability of this technique for studying the effect such platforms render on neonatal rat ventricular myocytes (NRVMs). Firstly, we have characterised topographically and mechanically the scaffolds in liquid at the nano-scale level. We thus establish that such platforms have physical properties that closely mimics the in vivo extracellular environment of cells. We have then studied the cell morphology and physiology by comparing cultures on flat uniformly-covered and collagen-patterned scaffolds. We show that micro-patterns promote the elongation of cells along the principal axis of the ridges coated with collagen. In several cases, cells also tend to create bridges across the grooves. We have finally studied cell contraction, monitoring Ca2+ cycling at a certain stimulation. Cells seeded on patterned scaffolds present significant responses in comparison to the isotropic ones.
AU  - Sanzari,I
AU  - Humphrey,EJ
AU  - Dinelli,F
AU  - Terracciano,CM
AU  - Prodromakis,T
DO  - 10.1038/s41598-018-30360-6
PY  - 2018///
SN  - 2045-2322
TI  - Effect of patterned polyacrylamide hydrogel on morphology and orientation of cultured NRVMs
T2  - Scientific Reports
UR  - http://dx.doi.org/10.1038/s41598-018-30360-6
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000441300700035&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/62845
VL  - 8
ER  -
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