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@article{Poulet:2021:cvr/cvaa033,
author = {Poulet, C and Sanchez-Alonso, J and Swiatlowska, P and Mouy, F and Lucarelli, C and Alvarez-Laviada, A and Terracciano, C and Houser, S and Gorelik, J},
doi = {cvr/cvaa033},
journal = {Cardiovascular Research},
pages = {149--161},
title = {Junctophilin-2 tethers T-tubules and recruits functional L-type calcium channels to lipid rafts in adult cardiomyocytes},
url = {http://dx.doi.org/10.1093/cvr/cvaa033},
volume = {117},
year = {2021}
}

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TY  - JOUR
AB  - Aim: In cardiomyocytes, transverse tubules (T-tubules) associate with the sarcoplasmic reticulum (SR), forming junctional membrane complexes (JMCs) where L-type calcium channels (LTCCs) are juxtaposed to Ryanodine receptors (RyR). Junctophilin-2 (JPH2) supports the assembly of JMCs by tethering T-tubules to the SR membrane. T-tubule remodeling in cardiac diseases is associated with down-regulation of JPH2 expression suggesting that JPH2 plays a crucial role in T-tubule stability. Furthermore, increasing evidence indicate that JPH2 might additionally act as a modulator of calcium signaling by directly regulating RyR and LTCCs. This study aimed at determining whether JPH2 overexpression restores normal T-tubule structure and LTCC function in cultured cardiomyocytes.Methods and results: Rat ventricular myocytes kept in culture for 4 days showed extensive T-tubule remodeling with impaired JPH2 localization and relocation of the scaffolding protein Caveolin3 (Cav3) from the T-tubules to the outer membrane. Overexpression of JPH2 restored T-tubule structure and Cav3 relocation. Depletion of membrane cholesterol by chronic treatment with Methyl-β-cyclodextrin (MβCD) countered the stabilizing effect of JPH2 overexpression on T-tubules and Cav3. Super-resolution scanning patch-clamp showed that JPH2 overexpression greatly increased the number of functional LTCCs at the plasma membrane. Treatment with MβCD reduced LTCC open probability and activity. Proximity ligation assays showed that MβCD did not affect JPH2 interaction with RyR and the pore-forming LTCC subunit Cav1.2, but strongly impaired JPH2 association with Cav3 and the accessory LTCC subunit Cavβ2. Conclusions: JPH2 promotes T-tubule structural stability and recruits functional LTCCs to the membrane, most likely by directly binding to the channel. Cholesterol is involved in the binding of JPH2 to T-tubules as well as in the modulation of LTCC activity. We propose a model where cholesterol an
AU  - Poulet,C
AU  - Sanchez-Alonso,J
AU  - Swiatlowska,P
AU  - Mouy,F
AU  - Lucarelli,C
AU  - Alvarez-Laviada,A
AU  - Terracciano,C
AU  - Houser,S
AU  - Gorelik,J
DO  - cvr/cvaa033
EP  - 161
PY  - 2021///
SN  - 0008-6363
SP  - 149
TI  - Junctophilin-2 tethers T-tubules and recruits functional L-type calcium channels to lipid rafts in adult cardiomyocytes
T2  - Cardiovascular Research
UR  - http://dx.doi.org/10.1093/cvr/cvaa033
UR  - http://hdl.handle.net/10044/1/77377
VL  - 117
ER  -

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