Imperial College London
Alternate

ProfessorCharlotteBevan

Faculty of Medicine, Department of Surgery & Cancer

Professor of Cancer Biology
 
 
 
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Contact

 

charlotte.bevan Website

 
 
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Assistant

 

Mrs Suzy Ford +44 (0)20 7594 2135

 
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Location

 

139ICTEM buildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Leach:2016:10.1016/j.mce.2016.09.010,
author = {Leach, DA and Panagopoulos, V and Nash, C and Bevan, C and Thomson, AA and Selth, LA and Buchanan, G},
doi = {10.1016/j.mce.2016.09.010},
journal = {Molecular and Cellular Endocrinology},
pages = {261--272},
title = {Cell-lineage specificity and role of AP-1 in the prostate fibroblast androgen receptor cistrome.},
url = {http://dx.doi.org/10.1016/j.mce.2016.09.010},
volume = {439},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Androgen receptor (AR) signalling in fibroblasts is important in prostate development and carcinogenesis, and is inversely related to prostate cancer mortality. However, the molecular mechanisms of AR action in fibroblasts and other non-epithelial cell types are largely unknown. The genome-wide DNA binding profile of AR in human prostate fibroblasts was identified by chromatin immunoprecipitation sequencing (ChIP-Seq), and found to be common to other fibroblast lines but disparate from AR cistromes of prostate cancer cells and tissue. Although AR binding sites specific to fibroblasts were less well conserved evolutionarily than those shared with cancer epithelia, they were likewise correlated with androgen regulation of fibroblast gene expression. Whereas FOXA1 is the key pioneer factor of AR in cancer epithelia, our data indicated that AP-1 likely plays a more important role in the AR cistrome in fibroblasts. The specificity of AP-1 and FOXA1 to binding in these cells is demonstrated using immunoblot and immunohistochemistry. Importantly, we find the fibroblast cistrome is represented in whole tissue/in vivo ChIP-seq studies at both genomic and resulting protein levels, highlighting the importance of the stroma in whole tissue -omic studies. This is the first nuclear receptor ChIP-seq study in prostatic fibroblasts, and provides novel insight into the action of fibroblast AR in prostate cancer.
AU  - Leach,DA
AU  - Panagopoulos,V
AU  - Nash,C
AU  - Bevan,C
AU  - Thomson,AA
AU  - Selth,LA
AU  - Buchanan,G
DO  - 10.1016/j.mce.2016.09.010
EP  - 272
PY  - 2016///
SN  - 1872-8057
SP  - 261
TI  - Cell-lineage specificity and role of AP-1 in the prostate fibroblast androgen receptor cistrome.
T2  - Molecular and Cellular Endocrinology
UR  - http://dx.doi.org/10.1016/j.mce.2016.09.010
UR  - http://hdl.handle.net/10044/1/43525
VL  - 439
ER  -
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