Imperial College London
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ProfessorChristianSpeck

Faculty of Medicine, Institute of Clinical Sciences

Professor of Genome Biochemistry & Molecular Biology
 
 
 
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Contact

 

+44 (0)7961 815 557chris.speck Website CV

 
 
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Location

 

2.14BLMS BuildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Tognetti:2014:10.1007/s00412-014-0489-2,
author = {Tognetti, S and Riera, A and Speck, C},
doi = {10.1007/s00412-014-0489-2},
journal = {Chromosoma},
pages = {13--26},
title = {Switch on the engine: how the eukaryotic replicative helicase MCM2–7 becomes activated},
url = {http://dx.doi.org/10.1007/s00412-014-0489-2},
volume = {124},
year = {2014}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - A crucial step during eukaryotic initiation of DNA replication is the correct loading and activation of the replicative DNA helicase, which ensures that each replication origin fires only once. Unregulated DNA helicase loading and activation, as it occurs in cancer, can cause severe DNA damage and genomic instability. The essential mini-chromosome maintenance proteins 2–7 (MCM2–7) represent the core of the eukaryotic replicative helicase that is loaded at DNA replication origins during G1-phase of the cell cycle. The MCM2–7 helicase activity, however, is only triggered during S-phase once the holo-helicase Cdc45-MCM2–7-GINS (CMG) has been formed. A large number of factors and several kinases interact and contribute to CMG formation and helicase activation, though the exact mechanisms remain unclear. Crucially, upon DNA damage, this reaction is temporarily halted to ensure genome integrity. Here, we review the current understanding of helicase activation; we focus on protein interactions during CMG formation, discuss structural changes during helicase activation, and outline similarities and differences of the prokaryotic and eukaryotic helicase activation process.
AU  - Tognetti,S
AU  - Riera,A
AU  - Speck,C
DO  - 10.1007/s00412-014-0489-2
EP  - 26
PY  - 2014///
SN  - 1432-0886
SP  - 13
TI  - Switch on the engine: how the eukaryotic replicative helicase MCM2–7 becomes activated
T2  - Chromosoma
UR  - http://dx.doi.org/10.1007/s00412-014-0489-2
UR  - http://hdl.handle.net/10044/1/27085
VL  - 124
ER  -
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