Imperial College London
Alternate

DrDavidAanensen

Faculty of Medicine, School of Public Health

Visiting Professor
 
 
 
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Contact

 

+44 (0)20 7594 3896d.aanensen Website

 
 
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Location

 

G30Norfolk PlaceSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Otter:2017:10.1038/s41598-017-12637-4,
author = {Otter, JA and Doumith, M and Davies, F and Mookerjee, S and Dyakova, E and Gilchrist, M and Brannigan, E and Bamford, K and Galletly, T and Donaldson, H and Aanensen, D and Ellington, M and Hill, R and Turton, J and Hopkins, K and Woodford, N and Holmes, AH},
doi = {10.1038/s41598-017-12637-4},
journal = {Scientific Reports},
title = {Emergence and clonal spread of colistin resistance due to multiple mutational mechanisms in carbapenemase-producing Klebsiella pneumoniae in London},
url = {http://dx.doi.org/10.1038/s41598-017-12637-4},
volume = {7},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Carbapenemase-producing Enterobacteriaceae (CPE) are emerging worldwide, limiting therapeutic options. Mutational and plasmid-mediated mechanisms of colistin resistance have both been reported. The emergence and clonal spread of colistin resistance was analysed in 40 epidemiologically-related NDM-1 carbapenemase producing Klebsiella pneumoniae isolates identified during an outbreak in a group of London hospitals. Isolates from July 2014 to October 2015 were tested for colistin susceptibility using agar dilution, and characterised by whole genome sequencing (WGS). Colistin resistance was detected in 25/38 (65.8%) cases for which colistin susceptibility was tested. WGS found that three potential mechanisms of colistin resistance had emerged separately, two due to different mutations in mgrB, and one due to a mutation in phoQ, with onward transmission of two distinct colistin-resistant variants, resulting in two sub-clones associated with transmission at separate hospitals. A high rate of colistin resistance (66%) emerged over a 10 month period. WGS demonstrated that mutational colistin resistance emerged three times during the outbreak, with transmission of two colistin-resistant variants.
AU  - Otter,JA
AU  - Doumith,M
AU  - Davies,F
AU  - Mookerjee,S
AU  - Dyakova,E
AU  - Gilchrist,M
AU  - Brannigan,E
AU  - Bamford,K
AU  - Galletly,T
AU  - Donaldson,H
AU  - Aanensen,D
AU  - Ellington,M
AU  - Hill,R
AU  - Turton,J
AU  - Hopkins,K
AU  - Woodford,N
AU  - Holmes,AH
DO  - 10.1038/s41598-017-12637-4
PY  - 2017///
SN  - 2045-2322
TI  - Emergence and clonal spread of colistin resistance due to multiple mutational mechanisms in carbapenemase-producing Klebsiella pneumoniae in London
T2  - Scientific Reports
UR  - http://dx.doi.org/10.1038/s41598-017-12637-4
UR  - http://hdl.handle.net/10044/1/50770
VL  - 7
ER  -
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