Imperial College London
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ProfessorDariusArmstrong-James

Faculty of Medicine, Department of Infectious Disease

Professor of Infectious Diseases and Medical Mycology
 
 
 
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Contact

 

d.armstrong

 
 
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Location

 

Flowers buildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Bidula:2015:10.1111/imm.12501,
author = {Bidula, S and Sexton, DW and Yates, M and Abdolrasouli, A and Shah, A and Wallis, R and Reed, A and Armstrong-James, D and Schelenz, S},
doi = {10.1111/imm.12501},
journal = {Immunology},
pages = {281--291},
title = {H-ficolin binds Aspergillus fumigatus leading to activation of the lectin complement pathway and modulation of lung epithelial immune responses.},
url = {http://dx.doi.org/10.1111/imm.12501},
volume = {146},
year = {2015}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Aspergillus fumigatus is an opportunistic fungal pathogen that typically infects the lungs of immunocompromised patients leading to a high mortality. H-ficolin, an innate immune opsonin, is produced by type II alveolar epithelial cells and could participate in lung defences against infections. Here, we utilised the human type II alveolar epithelial cell line, A549, to determine the involvement of H-ficolin in fungal defence. Additionally, we investigated the presence of H-ficolin in bronchoalveolar lavage fluid (BAL) from transplant patients during pneumonia. H-ficolin exhibited demonstrable binding to A. fumigatus conidia via L-fucose, D-mannose and N-acetylglucosamine residues in a calcium- and pH-dependent manner. Moreover, recognition led to lectin complement pathway activation and enhanced fungal association with A549 cells. Following recognition, H-ficolin opsonization manifested an increase in IL-8 production from A549 cells which involved activation of the intracellular signalling pathways MEK 1/2, p38 MAPK and JNK. Finally, H-ficolin concentrations were significantly higher in BAL of patients with lung infections compared to control subjects (n=16; p=0.00726). ROC curve analysis further highlighted the potential of H-ficolin as a diagnostic marker for lung infection (AUC=0.77; p<0.0001). Thus, H-ficolin participates in A. fumigatus defence via activation of the lectin complement pathway, enhanced fungal-host interactions and modulated immune responses. This article is protected by copyright. All rights reserved.
AU  - Bidula,S
AU  - Sexton,DW
AU  - Yates,M
AU  - Abdolrasouli,A
AU  - Shah,A
AU  - Wallis,R
AU  - Reed,A
AU  - Armstrong-James,D
AU  - Schelenz,S
DO  - 10.1111/imm.12501
EP  - 291
PY  - 2015///
SN  - 1365-2567
SP  - 281
TI  - H-ficolin binds Aspergillus fumigatus leading to activation of the lectin complement pathway and modulation of lung epithelial immune responses.
T2  - Immunology
UR  - http://dx.doi.org/10.1111/imm.12501
UR  - http://hdl.handle.net/10044/1/24966
VL  - 146
ER  -
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