Imperial College London
Alternate

Professor Daqing Ma, MD, PhD

Faculty of Medicine, Department of Surgery & Cancer

Professor of Anaesthesia
 
 
 
//

Contact

 

+44 (0)20 3315 8495d.ma Website

 
 
//

Assistant

 

Miss Steffi Klier +44 (0)20 3315 8816

 
//

Location

 

G3.44Chelsea and Westminster HospitalChelsea and Westminster Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Li:2019:10.1007/s00540-018-2577-6,
author = {Li, T and Chen, L and Zhao, H and Wu, L and Masters, J and Han, C and Hirota, K and Ma, D},
doi = {10.1007/s00540-018-2577-6},
journal = {Journal of Anesthesia},
pages = {17--25},
title = {Both Bupivacaine and Levobupivacaine inhibit colon cancer cell growth but not melanoma cells in vitro},
url = {http://dx.doi.org/10.1007/s00540-018-2577-6},
volume = {33},
year = {2019}
}
Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - BackgroundRetrospective studies indicate that the use of regional anaesthesia causes a reduction in cancer recurrence after oncological surgery, which could be due to anaesthetic’s negating effect on immunosuppression related to the surgical stress response. Local anaesthetics may also exert direct suppressive effects on malignant cells, an area where further investigation is urgently needed.MethodsHuman colon cancer cells and human melanoma cells were cultured and then treated with 1 mM bupivacaine or levobupivacaine for up to 24 or 48 h. Their migratory ability was measured by scratch assay, proliferation determined with Ki67 immunofluorescence staining, and apoptosis accessed with annexin V and PI staining on flow cytometry. The effects of bupivacaine and levobupivacaine on cellular signaling and molecular response, specifically, on endoplasmic reticulum stress (ERS), were studied with immunostaining and western blot.ResultsIn colon cancer cells, treatment with bupivacaine and levobupivacaine significantly inhibited cell migration (p < 0.01, p < 0.001; n = 4) and proliferation (p < 0.01; n = 4), while increasing the expression of CHOP (p < 0.001; n = 4) and decreased the expression of Grp78 (p < 0.05; n = 4). These effects were not mirrored by melanoma cells, such that no significant increase in apoptosis was seen in either melanoma cell lines following treatment.ConclusionThese in vitro data suggested that both bupivacaine and levobupivacaine suppress colorectal adenocarcinoma cell proliferation and migration, which are concurrent with increased endoplasmic reticulum stress. Conversely, melanoma cells are more resilient to these two commonly used local anaesthetics. Further in vivo studies or clinical trials are needed.
AU  - Li,T
AU  - Chen,L
AU  - Zhao,H
AU  - Wu,L
AU  - Masters,J
AU  - Han,C
AU  - Hirota,K
AU  - Ma,D
DO  - 10.1007/s00540-018-2577-6
EP  - 25
PY  - 2019///
SN  - 0913-8668
SP  - 17
TI  - Both Bupivacaine and Levobupivacaine inhibit colon cancer cell growth but not melanoma cells in vitro
T2  - Journal of Anesthesia
UR  - http://dx.doi.org/10.1007/s00540-018-2577-6
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000457989100003&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/75154
VL  - 33
ER  -
Download