Imperial College London
Alternate

Professor Daqing Ma, MD, PhD

Faculty of Medicine, Department of Surgery & Cancer

Professor of Anaesthesia
 
 
 
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Contact

 

+44 (0)20 3315 8495d.ma Website

 
 
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Assistant

 

Miss Steffi Klier +44 (0)20 3315 8816

 
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Location

 

G3.44Chelsea and Westminster HospitalChelsea and Westminster Campus

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Summary

 

Publications

Citation

BibTex format

@article{Zhao:2018:10.1111/ajt.14699,
author = {Zhao, H and Ma, D and Huang, H and Alam, A and Chen, Q and Suen, KC and Cui, J and Sun, Q and Ologunde, R and Zhang, W and Lian, Q},
doi = {10.1111/ajt.14699},
journal = {American Journal of Transplantation},
pages = {1890--1903},
title = {VEGF mitigates histone induced pyroptosis in the remote liver injury associated with renal allograft ischemia-reperfusion injury in rats},
url = {http://dx.doi.org/10.1111/ajt.14699},
volume = {18},
year = {2018}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Clinical evidence indicated a possible link between renal injury and remote liver injury. Herein, we investigated whether extracellular histone mediates remote hepatic damage following renal graft ischemia-reperfusion injury, whilst vascular endothelial growth factor (VEGF) is protective against remote hepatic injury. In vitro, hepatocyte HepG2 cultures were treated with histone. In vivo, the Brown-Norway renal graft was stored in 4°C preserving solution for 24 hours and then transplanted into Lewis rat recipient; blood samples and livers from recipients were harvested 24 hours after surgery. Prolonged cold ischemia in renal grafts enhanced liver injury 24 hours after engraftment. Caspase-1, ASC, NLRP3 and AIM2 expression in hepatocyte, CD68+ infiltrating macrophages, tissue and serum IL-1β and IL-18 were greatly elevated, indicating that pyroptosis occurred in the liver and resulted in acute liver functional impairment. Blocking caspase-1 pathway decreased the number of necrotic hepatocytes. VEGF treatment suppressed the hepatocyte pyroptosis and liver function was partially restored. Our data suggested that renal allograft ischemia-reperfusion injury is likely associated with acute liver damage due to hepatocyte pyroptosis induced by histone and such injury may be protected by VEGF administration. VEGF, therefore, may serve as a new strategy against other remote organ injuries related to renal transplant.
AU  - Zhao,H
AU  - Ma,D
AU  - Huang,H
AU  - Alam,A
AU  - Chen,Q
AU  - Suen,KC
AU  - Cui,J
AU  - Sun,Q
AU  - Ologunde,R
AU  - Zhang,W
AU  - Lian,Q
DO  - 10.1111/ajt.14699
EP  - 1903
PY  - 2018///
SN  - 1600-6135
SP  - 1890
TI  - VEGF mitigates histone induced pyroptosis in the remote liver injury associated with renal allograft ischemia-reperfusion injury in rats
T2  - American Journal of Transplantation
UR  - http://dx.doi.org/10.1111/ajt.14699
UR  - http://hdl.handle.net/10044/1/57239
VL  - 18
ER  -
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