Imperial College London
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ProfessorDarrylOverby

Faculty of Engineering, Department of Bioengineering

Professor of Mechanobiology
 
 
 
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Contact

 

+44 (0)20 7594 6376d.overby

 
 
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Location

 

3.07Bessemer BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Tam:2017:10.1038/srep40717,
author = {Tam, LC and Reina-Torres, E and Sherwood, JM and Cassidy, PS and Crosbie, DE and Lütjen-Drecoll, E and Flügel-Koch, C and Perkumas, K and Humphries, MM and Kiang, AS and O'Callaghan, J and Callanan, JJ and Read, AT and Ethier, CR and O'Brien, C and Lawrence, M and Campbell, M and Stamer, WD and Overby, DR and Humphries, P},
doi = {10.1038/srep40717},
journal = {Scientific Reports},
title = {Enhancement of outflow facility in the murine eye by targeting selected tight-junctions of Schlemm's canal endothelia},
url = {http://dx.doi.org/10.1038/srep40717},
volume = {7},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The juxtacanalicular connective tissue of the trabecular meshwork together with inner wall endothelium of Schlemm’s canal (SC) provide the bulk of resistance to aqueous outflow from the anterior chamber. Endothelial cells lining SC elaborate tight junctions (TJs), down-regulation of which may widen paracellular spaces between cells, allowing greater fluid outflow. We observed significant increase in paracellular permeability following siRNA-mediated suppression of TJ transcripts, claudin-11, zonula-occludens-1 (ZO-1) and tricellulin in human SC endothelial monolayers. In mice claudin-11 was not detected, but intracameral injection of siRNAs targeting ZO-1 and tricellulin increased outflow facility significantly. Structural qualitative and quantitative analysis of SC inner wall by transmission electron microscopy revealed significantly more open clefts between endothelial cells treated with targeting, as opposed to non-targeting siRNA. These data substantiate the concept that the continuity of SC endothelium is an important determinant of outflow resistance, and suggest that SC endothelial TJs represent a specific target for enhancement of aqueous movement through the conventional outflow system.
AU  - Tam,LC
AU  - Reina-Torres,E
AU  - Sherwood,JM
AU  - Cassidy,PS
AU  - Crosbie,DE
AU  - Lütjen-Drecoll,E
AU  - Flügel-Koch,C
AU  - Perkumas,K
AU  - Humphries,MM
AU  - Kiang,AS
AU  - O'Callaghan,J
AU  - Callanan,JJ
AU  - Read,AT
AU  - Ethier,CR
AU  - O'Brien,C
AU  - Lawrence,M
AU  - Campbell,M
AU  - Stamer,WD
AU  - Overby,DR
AU  - Humphries,P
DO  - 10.1038/srep40717
PY  - 2017///
SN  - 2045-2322
TI  - Enhancement of outflow facility in the murine eye by targeting selected tight-junctions of Schlemm's canal endothelia
T2  - Scientific Reports
UR  - http://dx.doi.org/10.1038/srep40717
UR  - http://www.ncbi.nlm.nih.gov/pubmed/28091584
UR  - http://hdl.handle.net/10044/1/44107
VL  - 7
ER  -
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