Imperial College London
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ProfessorDarrylOverby

Faculty of Engineering, Department of Bioengineering

Professor of Mechanobiology
 
 
 
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Contact

 

+44 (0)20 7594 6376d.overby

 
 
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Location

 

3.07Bessemer BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Chang:2017:10.1002/adhm.201700383,
author = {Chang, JYH and Chow, LW and Dismuke, WM and Ethier, CR and Stevens, MM and Stamer, WD and Overby, D},
doi = {10.1002/adhm.201700383},
journal = {Advanced Healthcare Materials},
title = {Peptide-functionalized fluorescent particles for in situ detection of nitric oxide via peroxynitrite-mediated nitration},
url = {http://dx.doi.org/10.1002/adhm.201700383},
volume = {6},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Nitric oxide (NO) is a free radical signaling molecule that plays a crucial role in modulating physiological homeostasis across multiple biological systems. NO dysregulation is linked to the pathogenesis of multiple diseases; therefore, its quantification is important for understanding pathophysiological processes. The detection of NO is challenging, typically limited by its reactive nature and short half-life. Additionally, the presence of interfering analytes and accessibility to biological fluids in the native tissues make the measurement technically challenging and often unreliable. Here, a bio-inspired peptide-based NO sensor is developed, which detects NO-derived oxidants, predominately peroxynitrite-mediated nitration of tyrosine residues. It is demonstrated that these peptide-based NO sensors can detect peroxynitrite-mediated nitration in response to physiological shear stress by endothelial cells in vitro. Using the peptide-conjugated fluorescent particle immunoassay, peroxynitrite-mediated nitration activity with a detection limit of ≈100 × 10−9m is detected. This study envisions that the NO detection platform can be applied to a multitude of applications including monitoring of NO activity in healthy and diseased tissues, localized detection of NO production of specific cells, and cell-based/therapeutic screening of peroxynitrite levels to monitor pronitroxidative stress in biological samples.
AU  - Chang,JYH
AU  - Chow,LW
AU  - Dismuke,WM
AU  - Ethier,CR
AU  - Stevens,MM
AU  - Stamer,WD
AU  - Overby,D
DO  - 10.1002/adhm.201700383
PY  - 2017///
SN  - 2192-2640
TI  - Peptide-functionalized fluorescent particles for in situ detection of nitric oxide via peroxynitrite-mediated nitration
T2  - Advanced Healthcare Materials
UR  - http://dx.doi.org/10.1002/adhm.201700383
UR  - http://hdl.handle.net/10044/1/48112
VL  - 6
ER  -
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