Imperial College London
Alternate

Dr Dina Vlachou

Faculty of Natural Sciences, Department of Life Sciences

Advanced Research Fellow
 
 
 
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Contact

 

+44 (0)20 7594 1267d.vlachou Website

 
 
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Location

 

612Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Bushell:2009:10.1371/journal.ppat.1000539,
author = {Bushell, ESC and Ecker, A and Schlegelmilch, T and Goulding, D and Dougan, G and Sinden, RE and Christophides, GK and Kafatos, FC and Vlachou, D},
doi = {10.1371/journal.ppat.1000539},
journal = {PLoS Pathogens},
pages = {1--13},
title = {Paternal effect of the nuclear formin-like protein MISFIT on plasmodium development in then mosquito vector},
url = {http://dx.doi.org/10.1371/journal.ppat.1000539},
volume = {5},
year = {2009}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Malaria parasites must undergo sexual and sporogonic development in mosquitoes before they can infect their vertebrate hosts. We report the discovery and characterization of MISFIT, the first protein with paternal effect on the development of the rodent malaria parasite Plasmodium berghei in Anopheles mosquitoes. MISFIT is expressed in male gametocytes and localizes to the nuclei of male gametocytes, zygotes and ookinetes. Gene disruption results in mutant ookinetes with reduced genome content, microneme defects and altered transcriptional profiles of putative cell cycle regulators, which yet successfully invade the mosquito midgut. However, developmental arrest ensues during the ookinete transformation to oocysts leading to malaria transmission blockade. Genetic crosses between misfit mutant parasites and parasites that are either male or female gamete deficient reveal a strict requirement for a male misfit allele. MISFIT belongs to the family of formin-like proteins, which are known regulators of the dynamic remodeling of actin and microtubule networks. Our data identify the ookinete-to-oocyst transition as a critical cell cycle checkpoint in Plasmodium development and lead us to hypothesize that MISFIT may be a regulator of cell cycle progression. This study offers a new perspective for understanding the male contribution to malaria parasite development in the mosquito vector.
AU  - Bushell,ESC
AU  - Ecker,A
AU  - Schlegelmilch,T
AU  - Goulding,D
AU  - Dougan,G
AU  - Sinden,RE
AU  - Christophides,GK
AU  - Kafatos,FC
AU  - Vlachou,D
DO  - 10.1371/journal.ppat.1000539
EP  - 13
PY  - 2009///
SN  - 1553-7366
SP  - 1
TI  - Paternal effect of the nuclear formin-like protein MISFIT on plasmodium development in then mosquito vector
T2  - PLoS Pathogens
UR  - http://dx.doi.org/10.1371/journal.ppat.1000539
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000270804500024&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1000539
UR  - http://hdl.handle.net/10044/1/91810
VL  - 5
ER  -
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