Imperial College London
Alternate

Professor Daniel Elson

Faculty of Medicine, Department of Surgery & Cancer

Professor of Surgical Imaging
 
 
 
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Contact

 

+44 (0)20 7594 1700daniel.elson Website CV

 
 
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Location

 

415 Bessemer BuildingBessemer BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Ong:2017:10.1021/acsami.7b14851,
author = {Ong, ZY and Chen, S and Nabavi, E and Regoutz, A and Payne, DJ and Elson, DS and Dexter, DT and Dunlop, IE and Porter, AE},
doi = {10.1021/acsami.7b14851},
journal = {ACS Applied Materials and Interfaces},
pages = {39259--39270},
title = {Multibranched Gold Nanoparticles with Intrinsic LAT-1 Targeting Capabilities for Selective Photothermal Therapy of Breast Cancer.},
url = {http://dx.doi.org/10.1021/acsami.7b14851},
volume = {9},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Because of the critical role of the large neutral amino acid transporter-1 (LAT-1) in promoting tumor growth and proliferation, it is fast emerging as a highly attractive biomarker for the imaging and treatment of human malignancies, including breast cancer. While multibranched gold nanoparticles (AuNPs) have emerged as a promising modality in the photothermal therapy (PTT) of cancers, some of the key challenges limiting their clinical translation lie in the need to develop reproducible and cost-effective synthetic methods as well as the selective accumulation of sufficient AuNPs at tumor sites. In this study, we report a simple and direct seed-mediated synthesis of monodispersed multibranched AuNPs using the catechol-containing LAT-1 ligands, L- and D-dopa, to confer active cancer targeting. This route obviates the need for additional conjugation with targeting moieties such as peptides or antibodies. Nanoflower-like AuNPs (AuNF) with diameters of approximately 46, 70, and 90 nm were obtained and were found to possess excellent colloidal stability and biocompatibility. A significantly higher intracellular accumulation of the L- and D-dopa functionalized AuNFs was observed in a panel of breast cancer cell lines (MCF-7, MDA-MB-231, MDA-MB-468, and MDA-MB-453) when compared to the nontargeting control AuNFs synthesized with dopamine and 4-ethylcatechol. Importantly, no significant difference in uptake between the targeting and nontargeting AuNFs was observed in a non-tumorigenic MCF-10A breast epithelial cell line, hence demonstrating tumor selectivity. For PTT of breast cancer, Ag(+) was introduced during synthesis to obtain L-dopa functionalized nanourchin-like AuNPs (AuNUs) with strong near-infrared (NIR) absorbance. The L-dopa functionalized AuNUs mediated selective photothermal ablation of the triple negative MDA-MB-231 breast cancer cell line and sensitized the cells to the anticancer drugs cisplatin and docetaxel. This work brings forward an effective strategy
AU  - Ong,ZY
AU  - Chen,S
AU  - Nabavi,E
AU  - Regoutz,A
AU  - Payne,DJ
AU  - Elson,DS
AU  - Dexter,DT
AU  - Dunlop,IE
AU  - Porter,AE
DO  - 10.1021/acsami.7b14851
EP  - 39270
PY  - 2017///
SN  - 1944-8244
SP  - 39259
TI  - Multibranched Gold Nanoparticles with Intrinsic LAT-1 Targeting Capabilities for Selective Photothermal Therapy of Breast Cancer.
T2  - ACS Applied Materials and Interfaces
UR  - http://dx.doi.org/10.1021/acsami.7b14851
UR  - http://hdl.handle.net/10044/1/54689
VL  - 9
ER  -
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