Imperial College London

ProfessorDavidCarling

Faculty of MedicineInstitute of Clinical Sciences

Professor of Biochemistry
 
 
 
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Contact

 

+44 (0)20 3313 4313david.carling

 
 
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Location

 

3rd Fl CRBHammersmith HospitalHammersmith Campus

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Summary

 

Publications

Publication Type
Year
to

154 results found

Zhao J, Carling D, Wang Z, Muckett P, Leiper J, Woods Aet al., 2013, Loss of CaMKK beta attenuates endotoxin induced hypotension, Spring Meeting for Clinician Scientists in Training, Publisher: ELSEVIER SCIENCE INC, Pages: 119-119, ISSN: 0140-6736

Conference paper

Johns M, Carling D, Piper EL, Mason JC, Haskard DO, Boyle JJet al., 2012, An Adenosine Monophosphate Activated Protein Kinase / Activating Transcription Factor-1 Cascade Initiates Commitment to Mhem Atheroprotective Macrophages in Response to Heme or Metformin, CIRCULATION, Vol: 126, ISSN: 0009-7322

Journal article

Laine R, Stuckey DW, Manning H, Warren SC, Kennedy G, Carling D, Dunsby C, Sardini A, French PMWet al., 2012, Fluorescence Lifetime Readouts of Troponin-C-Based Calcium FRET Sensors: A Quantitative Comparison of CFP and mTFP1 as Donor Fluorophores, PLOS ONE, Vol: 7, ISSN: 1932-6203

Journal article

Carling D, Thornton C, Woods A, Sanders MJet al., 2012, AMP-activated protein kinase: new regulation, new roles?, BIOCHEMICAL JOURNAL, Vol: 445, Pages: 11-27, ISSN: 0264-6021

Journal article

Oliveira SM, Zhang Y-H, Solis RS, Isackson H, Bellahcene M, Yavari A, Pinter K, Davies JK, Ge Y, Ashrafian H, Walker JW, Carling D, Watkins H, Casadei B, Redwood Cet al., 2012, AMP-Activated Protein Kinase Phosphorylates Cardiac Troponin I and Alters Contractility of Murine Ventricular Myocytes, CIRCULATION RESEARCH, Vol: 110, Pages: 1192-1201, ISSN: 0009-7330

Journal article

Tsuchiya Y, Denison FC, Heath RB, Carling D, Saggerson Det al., 2012, 5 '-AMP-activated protein kinase is inactivated by adrenergic signalling in adult cardiac myocytes, BIOSCIENCE REPORTS, Vol: 32, Pages: 197-209, ISSN: 0144-8463

Journal article

Fritah A, Steel JH, Parker N, Nikolopoulou E, Christian M, Carling D, Parker MGet al., 2012, Absence of RIP140 Reveals a Pathway Regulating glut4-Dependent Glucose Uptake in Oxidative Skeletal Muscle through UCP1-Mediated Activation of AMPK, PLOS ONE, Vol: 7, ISSN: 1932-6203

Journal article

Woods A, Leiper JM, Carling D, 2012, The role of ATM in response to metformin treatment and activation of AMPK., Nature Genetics, Vol: 44, Pages: 360-361

Journal article

Denison FC, Smith LB, Muckett PJ, O'Hara L, Carling D, Woods Aet al., 2011, LKB1 Is an Essential Regulator of Spermatozoa Release during Spermiation in the Mammalian Testis, PLOS ONE, Vol: 6, ISSN: 1932-6203

Journal article

Mayer FV, Heath R, Underwood E, Sanders MJ, Carmena D, McCartney RR, Leiper FC, Xiao B, Jing C, Walker PA, Haire LF, Ogrodowicz R, Martin SR, Schmidt MC, Gamblin SJ, Carling Det al., 2011, ADP regulates SNF1, the Saccharomyces cerevisiae homolog of AMP-activated protein kinase., Cell Metab, Vol: 14, Pages: 707-714

The SNF1 protein kinase complex plays an essential role in regulating gene expression in response to the level of extracellular glucose in budding yeast. SNF1 shares structural and functional similarities with mammalian AMP-activated protein kinase. Both kinases are activated by phosphorylation on a threonine residue within the activation loop segment of the catalytic subunit. Here we show that ADP is the long-sought metabolite that activates SNF1 in response to glucose limitation by protecting the enzyme against dephosphorylation by Glc7, its physiologically relevant protein phosphatase. We also show that the regulatory subunit of SNF1 has two ADP binding sites. The tighter site binds AMP, ADP, and ATP competitively with NADH, whereas the weaker site does not bind NADH, but is responsible for mediating the protective effect of ADP on dephosphorylation. Mutagenesis experiments suggest that the general mechanism by which ADP protects against dephosphorylation is strongly conserved between SNF1 and AMPK.

Journal article

Hardie DG, Carling D, Gamblin SJ, 2011, AMP-activated protein kinase: also regulated by ADP?, TRENDS IN BIOCHEMICAL SCIENCES, Vol: 36, Pages: 470-477, ISSN: 0968-0004

Journal article

Carling D, Mayer FV, Sanders MJ, Gamblin SJet al., 2011, AMP-activated protein kinase: nature's energy sensor, NATURE CHEMICAL BIOLOGY, Vol: 7, Pages: 512-518, ISSN: 1552-4450

Journal article

Xiao B, Sanders MJ, Underwood E, Heath R, Mayer FV, Carmena D, Jing C, Walker PA, Eccleston JF, Haire LF, Saiu P, Howell SA, Aasland R, Martin SR, Carling D, Gamblin SJet al., 2011, Structure of mammalian AMPK and its regulation by ADP, NATURE, Vol: 472, Pages: 230-233, ISSN: 0028-0836

Journal article

Claret M, Smith MA, Knauf C, Al-Qassab H, Woods A, Heslegrave A, Piipari K, Emmanuel JJ, Colom A, Valet P, Cani PD, Begum G, White A, Mucket P, Peters M, Mizuno K, Batterham RL, Giese KP, Ashworth A, Burcelin R, Ashford ML, Carling D, Withers DJet al., 2011, Deletion of Lkb1 in Pro-Opiomelanocortin Neurons Impairs Peripheral Glucose Homeostasis in Mice, DIABETES, Vol: 60, Pages: 735-745, ISSN: 0012-1797

Journal article

Woods A, Heslegrave AJ, Muckett PJ, Levene AP, Clements M, Mobberley M, Ryder TA, Abu-Hayyeh S, Williamson C, Goldin RD, Ashworth A, Withers DJ, Carling Det al., 2011, LKB1 is required for hepatic bile acid transport and canalicular membrane integrity in mice, BIOCHEMICAL JOURNAL, Vol: 434, Pages: 49-60, ISSN: 0264-6021

Journal article

Thorton C, Bright N, Sastre M, Muckett PJ, Carling Det al., 2011, AMP-activated protein kinase (AMPK) is a tau kinase, activated in response to β-amyloid exposure, Biochemical Journal, Vol: 434, Pages: 503-512

Journal article

Bungard D, Fuerth BJ, Zeng P-Y, Faubert B, Maas NL, Viollet B, Carling D, Thompson CB, Jones RG, Berger SLet al., 2010, Signaling Kinase AMPK Activates Stress-Promoted Transcription via Histone H2B Phosphorylation, SCIENCE, Vol: 329, Pages: 1201-1205, ISSN: 0036-8075

Journal article

Lopez M, Varela L, Vazquez MJ, Rodriguez-Cuenca S, Gonzalez CR, Velagapudi VR, Morgan DA, Schoenmakers E, Agassandian K, Lage R, Martinez de Morentin PB, Tovar S, Nogueiras R, Carling D, Lelliott C, Gallego R, Oresic M, Chatterjee K, Saha AK, Rahmouni K, Dieguez C, Vidal-Puig Aet al., 2010, Hypothalamic AMPK and fatty acid metabolism mediate thyroid regulation of energy balance, NATURE MEDICINE, Vol: 16, Pages: 1001-U97, ISSN: 1078-8956

Journal article

Beall C, Piipari K, Al-Qassab H, Smith MA, Parker N, Carling D, Viollet B, Withers DJ, Ashford MLJet al., 2010, Loss of AMP-activated protein kinase alpha 2 subunit in mouse beta-cells impairs glucose-stimulated insulin secretion and inhibits their sensitivity to hypoglycaemia, BIOCHEMICAL JOURNAL, Vol: 429, Pages: 323-333, ISSN: 0264-6021

Journal article

Piipari K, Beall C, Al-Qassab H, Smith MA, Carling D, Viollet B, Ashford MLJ, Withers DJet al., 2010, Key Role of AMP-Activated Protein Kinase in Pancreatic Beta Cell Glucose-Sensing and Whole Body Glucose Homeostasis, 92nd Meeting and Expo of the Endocrine Society (ENDO 2010), Publisher: ENDOCRINE SOC, Pages: S810-S810, ISSN: 0163-769X

Conference paper

Stahmann N, Woods A, Spengler K, Heslegrave A, Bauer R, Krause S, Viollet B, Carling D, Heller Ret al., 2010, Activation of AMP-activated Protein Kinase by Vascular Endothelial Growth Factor Mediates Endothelial Angiogenesis Independently of Nitric-oxide Synthase, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 285, Pages: 10638-10652

Journal article

Garcia-Garcia C, Fumarola C, Navaratnam N, Carling D, Lopez-Rivas Aet al., 2010, AMPK-independent down-regulation of cFLIP and sensitization to TRAIL-induced apoptosis by AMPK activators, BIOCHEMICAL PHARMACOLOGY, Vol: 79, Pages: 853-863, ISSN: 0006-2952

Journal article

Carling D, 2010, The role of the AMPK cascade in regulating energy metabolism, ENDOCRINE JOURNAL, Vol: 57, Pages: S250-S250, ISSN: 0918-8959

Journal article

Humbert N, Navaratnam N, Augert A, Costa MD, Martien S, Wang J, Martinez D, Abbadie C, Carling D, Launoit YD, Gil J, Bernard Det al., 2010, Regulation of ploidy and senescence by the AMPK-related kinase NUAK1, EMBO J, Vol: 29, Pages: 376-386

Senescence is an irreversible cell-cycle arrest that is elicited by a wide range of factors, including replicative exhaustion. Emerging evidences suggest that cellular senescence contributes to ageing and acts as a tumour suppressor mechanism. To identify novel genes regulating senescence, we performed a loss-of-function screen on normal human diploid fibroblasts. We show that downregulation of the AMPK-related protein kinase 5 (ARK5 or NUAK1) results in extension of the cellular replicative lifespan. Interestingly, the levels of NUAK1 are upregulated during senescence whereas its ectopic expression triggers a premature senescence. Cells that constitutively express NUAK1 suffer gross aneuploidies and show diminished expression of the genomic stability regulator LATS1, whereas depletion of NUAK1 with shRNA exerts opposite effects. Interestingly, a dominant-negative form of LATS1 phenocopies NUAK1 effects. Moreover, we show that NUAK1 phosphorylates LATS1 at S464 and this has a role in controlling its stability. In summary, our work highlights a novel role for NUAK1 in the control of cellular senescence and cellular ploidy. The EMBO Journal (2010) 29, 376-386. doi: 10.1038/emboj.2009.342; Published online 19 November 2009

Journal article

Selman C, Tullet JMA, Wieser D, Irvine E, Lingard SJ, Choudhury AI, Claret M, Al-Qassab H, Carmignac D, Ramadani F, Woods A, Robinson ICA, Schuster E, Batterham RL, Kozma SC, Thomas G, Carling D, Okkenhaug K, Thornton JM, Partridge L, Gems D, Withers DJet al., 2009, Ribosomal Protein S6 Kinase 1 Signaling Regulates Mammalian Life Span, SCIENCE, Vol: 326, Pages: 140-144, ISSN: 0036-8075

Journal article

Renz B, Davies JK, Carling D, Watkins H, Redwood Cet al., 2009, Determination of AMP-activated protein kinase phosphorylation sites in recombinant protein expressed using the pET28a vector: A cautionary tale, PROTEIN EXPRESSION AND PURIFICATION, Vol: 66, Pages: 181-184, ISSN: 1046-5928

Journal article

Bright NJ, Thornton C, Carling D, 2009, The regulation and function of mammalian AMPK-related kinases, ACTA PHYSIOLOGICA, Vol: 196, Pages: 15-26, ISSN: 1748-1708

Journal article

Martin MJ, Carling D, Marais R, 2009, Taking the Stress out of Melanoma, CANCER CELL, Vol: 15, Pages: 163-164, ISSN: 1535-6108

Journal article

Carling D, 2009, Branching out on AMPK Regulation, CELL METABOLISM, Vol: 9, Pages: 7-8, ISSN: 1550-4131

Journal article

Denison FC, Hiscock NJ, Carling D, Woods Aet al., 2009, Characterisation of an alternative splice variant of LKB1, J. Biol. Chem., Vol: 284, Pages: 67-76

Journal article

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