Imperial College London
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Dr Ernesto Cota

Faculty of Natural Sciences, Department of Life Sciences

Senior Lecturer
 
 
 
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Contact

 

+44 (0)20 7594 3689e.cota Website

 
 
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Location

 

601Sir Ernst Chain BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Pennington:2019:10.1371/journal.ppat.1007620,
author = {Pennington, HG and Rhian, J and Kwon, S and Bonciani, G and Thieron, H and Chandler, T and Luong, P and Morgan, S and Przydacz, M and Bozkurt, T and Wallington, E and Kwaaitaal, M and Panstruga, R and Cota, E and Spanu, P},
doi = {10.1371/journal.ppat.1007620},
journal = {PLoS Pathogens},
title = {The fungal ribonuclease-like effector protein CSEP0064/BEC1054 represses plant immunity and interferes with degradation of host ribosomal RNA},
url = {http://dx.doi.org/10.1371/journal.ppat.1007620},
volume = {15},
year = {2019}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The biotrophic fungal pathogen Blumeria graminis causes the powdery mildew disease of cereals and grasses. We present the first crystal structure of a B. graminis effector of pathogenicity (CSEP0064/BEC1054), demonstrating it has a ribonuclease (RNase)-like fold. This effector is part of a group of RNase-like proteins (termed RALPHs) which comprise the largest set of secreted effector candidates within the B. graminis genomes. Their exceptional abundance suggests they play crucial functions during pathogenesis. We show that transgenic expression of RALPH CSEP0064/BEC1054 increases susceptibility to infection in both monocotyledonous and dicotyledonous plants. CSEP0064/BEC1054 interacts in planta with the pathogenesis-related protein PR10. The effector protein associates with total RNA and weakly with DNA. Methyl jasmonate (MeJA) levels modulate susceptibility to aniline-induced host RNA fragmentation. In planta expression of CSEP0064/BEC1054 reduces the formation of this RNA fragment. We propose CSEP0064/BEC1054 is a pseudoenzyme that binds to host ribosomes, thereby inhibiting the action of plant ribosome-inactivating proteins (RIPs) that would otherwise lead to host cell death, an unviable interaction and demise of the fungus.
AU  - Pennington,HG
AU  - Rhian,J
AU  - Kwon,S
AU  - Bonciani,G
AU  - Thieron,H
AU  - Chandler,T
AU  - Luong,P
AU  - Morgan,S
AU  - Przydacz,M
AU  - Bozkurt,T
AU  - Wallington,E
AU  - Kwaaitaal,M
AU  - Panstruga,R
AU  - Cota,E
AU  - Spanu,P
DO  - 10.1371/journal.ppat.1007620
PY  - 2019///
SN  - 1553-7366
TI  - The fungal ribonuclease-like effector protein CSEP0064/BEC1054 represses plant immunity and interferes with degradation of host ribosomal RNA
T2  - PLoS Pathogens
UR  - http://dx.doi.org/10.1371/journal.ppat.1007620
UR  - http://hdl.handle.net/10044/1/67447
VL  - 15
ER  -
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