Imperial College London
Alternate

DrStathisGiotis

Faculty of Medicine, Department of Infectious Disease

Honorary Senior Research Fellow
 
 
 
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Contact

 

+44 (0)20 7594 9057e.giotis

 
 
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Location

 

Medical SchoolSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Giotis:2017:10.1038/s41598-017-17730-2,
author = {Giotis, ES and Ross, CS and Robey, RC and Nohturfft, A and Goodbourn, S and Skinner, MA},
doi = {10.1038/s41598-017-17730-2},
journal = {Scientific Reports},
title = {Constitutively elevated levels of SOCS1 suppress innate responses in DF-1 immortalised chicken fibroblast cells},
url = {http://dx.doi.org/10.1038/s41598-017-17730-2},
volume = {7},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The spontaneously immortalised DF-1 cell line is rapidly replacing its progenitor primary chicken embryo fibroblasts (CEFs) for studies on avian viruses such as avian influenza but no comprehensive study has as yet been reported comparing their innate immunity phenotypes. We conducted microarray analyses of DF-1 and CEFs, under both normal and stimulated conditions using chicken interferon-α (chIFNα) and the attenuated infectious bursal disease virus vaccine strain PBG98. We found that DF-1 have an attenuated innate response compared to CEFs. Basal expression levels of Suppressor of Cytokine Signalling 1 (chSOCS1), a negative regulator of cytokine signalling in mammals, are 16-fold higher in DF-1 than in CEFs. The chSOCS1 “SOCS box” domain (which, in mammals, interacts with an E3 ubiquitin ligase complex) is not essential for the inhibition of cytokine-induced JAK/STAT signalling activation in DF-1. Overexpression of SOCS1 in chIFNα-stimulated DF-1 led to a relative decrease in expression of interferon-stimulated genes (ISGs; MX1 and IFIT5) and increased viral yield in response to PBG98 infection. Conversely, knockdown of SOCS1 enhanced induction of ISGs and reduced viral yield in chIFNα-stimulated DF-1. Consequently, SOCS1 reduces induction of the IFN signalling pathway in chicken cells and can potentiate virus replication.
AU  - Giotis,ES
AU  - Ross,CS
AU  - Robey,RC
AU  - Nohturfft,A
AU  - Goodbourn,S
AU  - Skinner,MA
DO  - 10.1038/s41598-017-17730-2
PY  - 2017///
SN  - 2045-2322
TI  - Constitutively elevated levels of SOCS1 suppress innate responses in DF-1 immortalised chicken fibroblast cells
T2  - Scientific Reports
UR  - http://dx.doi.org/10.1038/s41598-017-17730-2
UR  - http://hdl.handle.net/10044/1/54412
VL  - 7
ER  -
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