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@article{Allott:2021:10.1039/d1ra02482e,
author = {Allott, L and Chen, C and Braga, M and Leung, SFJ and Wang, N and Barnes, C and Brickute, D and Carroll, L and Aboagye, EO},
doi = {10.1039/d1ra02482e},
journal = {RSC Advances: an international journal to further the chemical sciences},
pages = {20335--20341},
title = {Detecting hypoxia in vitro using 18F-pretargeted IEDDA “click” chemistry in live cells},
url = {http://dx.doi.org/10.1039/d1ra02482e},
volume = {11},
year = {2021}
}

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TY  - JOUR
AB  - We have exemplified a pretargeted approach to interrogate hypoxia in live cells using radioactive bioorthogonal inverse electron demand Diels–Alder (IEDDA) “click” chemistry. Our novel 18F-tetrazine probe ([18F]FB-Tz) and 2-nitroimidazole-based TCO targeting molecule (8) showed statistically significant (P < 0.0001) uptake in hypoxic cells (ca. 90 %ID per mg) vs. normoxic cells (<10 %ID per mg) in a 60 min incubation of [18F]FB-Tz. This is the first time that an intracellularly targeted small-molecule for IEDDA “click” has been used in conjunction with a radioactive reporter molecule in live cells and may be a useful tool with far-reaching applicability for a variety of applications.
AU  - Allott,L
AU  - Chen,C
AU  - Braga,M
AU  - Leung,SFJ
AU  - Wang,N
AU  - Barnes,C
AU  - Brickute,D
AU  - Carroll,L
AU  - Aboagye,EO
DO  - 10.1039/d1ra02482e
EP  - 20341
PY  - 2021///
SN  - 2046-2069
SP  - 20335
TI  - Detecting hypoxia in vitro using 18F-pretargeted IEDDA “click” chemistry in live cells
T2  - RSC Advances: an international journal to further the chemical sciences
UR  - http://dx.doi.org/10.1039/d1ra02482e
UR  - https://pubs.rsc.org/en/content/articlelanding/2021/RA/D1RA02482E#!divAbstract
UR  - http://hdl.handle.net/10044/1/89364
VL  - 11
ER  -

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