Imperial College London
Alternate

Professor Francis Drobniewski

Faculty of Medicine, Department of Infectious Disease

Chair in Global Health and Tuberculosis
 
 
 
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Contact

 

f.drobniewski

 
 
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Location

 

Commonwealth BuildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Chongwe:2018:10.1016/j.ijid.2018.03.020,
author = {Chongwe, G and Michelo, C and Sinkala, E and Kayamba, V and Nzayisenga, J-B and Drobniewski, F and Kelly, P},
doi = {10.1016/j.ijid.2018.03.020},
journal = {International Journal of Infectious Diseases},
pages = {73--81},
title = {Mycobacterium avium lysate induces matrix metalloproteinase-1 in intestinal tissue and peripheral blood: observations from selected hospital based Zambian adults},
url = {http://dx.doi.org/10.1016/j.ijid.2018.03.020},
volume = {71},
year = {2018}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - OBJECTIVES: Environmental enteropathy is prevalent in low-income countries, although its aetiology is unknown. We investigated if Mycobacterium avium antigens, which are commonly found in the environment, could contribute to its pathogenesis in a population known to have widespread environmental enteropathy. METHODS: Routine endoscopy patients at the University Teaching Hospital, Lusaka whose endoscopy results were normal submitted duodenal biopsies and whole blood samples. Samples were stimulated with M. avium lysate over 24hours while unstimulated samples served as negative controls. Matrix metalloproteinase (MMP) and cytokine response in supernatants were quantified using ELISA and cytometric bead array. RESULTS: Samples from 48 patients (56% women) were analysed, with a median age of 35 years (IQR 27.5, 50.5). M. avium induced the secretion of a wide-range of Th1, Th2 and Th17 cytokines in blood but only IL-1β and IL-6 in duodenal tissue. However it differentially induced the secretion of MMP-1 in duodenal tissue compared to negative controls (p=0.004). A similar MMP-1 response but with lower concentrations was observed in blood. CONCLUSION: The induction of MMP-1 and cytokines by M. avium in duodenal tissue suggests that environmental mycobacteria could contribute to the epithelial disruption seen in environmental enteropathy, and a need to further explore possible biomarkers that may predict this exposure in at-risk populations.
AU  - Chongwe,G
AU  - Michelo,C
AU  - Sinkala,E
AU  - Kayamba,V
AU  - Nzayisenga,J-B
AU  - Drobniewski,F
AU  - Kelly,P
DO  - 10.1016/j.ijid.2018.03.020
EP  - 81
PY  - 2018///
SN  - 1201-9712
SP  - 73
TI  - Mycobacterium avium lysate induces matrix metalloproteinase-1 in intestinal tissue and peripheral blood: observations from selected hospital based Zambian adults
T2  - International Journal of Infectious Diseases
UR  - http://dx.doi.org/10.1016/j.ijid.2018.03.020
UR  - https://www.ncbi.nlm.nih.gov/pubmed/29680481
UR  - http://hdl.handle.net/10044/1/58831
VL  - 71
ER  -
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