Publications
120 results found
Xie F, Ryan M, Riley J, et al., 2009, Investigation of homogeneous large surface area anodic alumina membrane synthesis, IEEE-NANO 2009. 9th IEEE Conference, Pages: 319-321
Gao Z, Xie F, Xie H, 2008, Determination of nucleic acid using electrocatalytic intercalators, US Patent 7,462,720
Xie F, Drozdowicz-Tomsia K, Goldys EM, 2008, A method to assess modifications of fluorophore radiative rate by plasmonic structures, Chemical Physics Letters, Vol: 466, Pages: 186-188, ISSN: 0009-2614
Xie F, Baker MS, Goldys EM, 2008, Enhanced Fluorescence Detection on Homogeneous Gold Colloid Self-Assembled Monolayer Substrates, Chemistry of Materials, Vol: 20, Pages: 1788-1797, ISSN: 0897-4756
Goldys EM, Drozdowicz-Tomsia K, Xie F, et al., 2007, Fluorescence Amplification by Electrochemically Deposited Silver Nanowires with Fractal Architecture, Journal of the American Chemical Society, Vol: 129, Pages: 12117-12122, ISSN: 0002-7863
Goldys EM, Barnett A, Xie F, et al., 2007, Plasmon-enhanced fluorescence near metallic nanostructures: biochemical applications, Applied Physics A, Vol: 89, Pages: 265-271, ISSN: 0947-8396
Goldys EM, Xie F, 2007, Metallic Nanomaterials for Sensitivity Enhancement of Fluorescence Detection, Sensors, Vol: 8, Pages: 886-896
Xie F, Goldys E, Baker M, 2007, Amplified protein sensing using deep purple fluorophores on homogeneous Au substrates, BioFactors, Vol: 30, Pages: 249-253
We report here the first observation of appreciable enhancement of fluorescence induced by large Au colloids. Au monolayers with Au colloids of 40 nm, 59 nm, and 81 nm in radii, were formed on silane modified glass surfaces, respectively. The nanoparticle densities were varied by varying the deposition times and documented by scanning electron microscopy. Two types of samples were prepared, with large inter-particle distance and hence little or no inter-particle coupling and small inter-particle distance with inter-particle coupling. The fluorescence enhancement was examined by using a self assembled monolayer of the fluorophore-protein conjugate with Deep Purple as a fluorophore and Bovine Serum Albumin as protein (DP-BSA). The data show the over 15 fold enhancement under optimized conditions and reveal strong variations with both inter-particle distance and particle size. Nanostructures of appropriate size with optimized inter-particle distance thus prepared can produce promising substrates for decent fluorescence enhancement. We demonstrated that the Au colloid monolayers on glass surfaces are promising substrates for fluorescence enhancement with outstanding macroscopic homogeneity. This important feature will pave the way for the application of our substrates in biotechnology and life sciences such as imaging and sensing of biomolecules in proteomics.
Xie F, Goldys E, Baker M, 2007, Enhanced fluorescence detection induced by homogeneous Au colloid monolayer substrates, International Conference on Cellular & Molecular Bioengineering (ICCMB)
Xie F, Goldys E, Baker M, 2007, Feasibility Study of Enhanced Fluorescence Detection by Au Colloid Modified Membrane for Electroblot, Proceedings of 4th International & 7th Australian Peptide Conference
Xie F, Baker MS, Goldys EM, 2006, Homogeneous Silver-Coated Nanoparticle Substrates for Enhanced Fluorescence Detection, The Journal of Physical Chemistry B, Vol: 110, Pages: 23085-23091, ISSN: 1520-6106
Gao Z, Xie F, Xie H, 2006, Metallocenes and processes for their preparation, US Patent 7,030,257
Xie F, Goldys E, 2006, Ag-coated Nanoparticles and their application on fluorescence enhancement, Australian Institute of Physics (AIP) Physics Industry Day
Xie F, Goldys E, 2006, The Effect of size of Au-core Ag-shell Nanoparticles on their Enhancement of Fluorescence, Proceedings of the 2006 International Conference on Nanoscience and Nanotechnology
Gao Z, Xie F, Shariff M, et al., 2005, A disposable glucose biosensor based on diffusional mediator dispersed in nanoparticulate membrane on screen-printed carbon electrode, Sensors and Actuators B: Chemical, Vol: 111-112, Pages: 339-346, ISSN: 0925-4005
Tansil NC, Xie F, Xie H, et al., 2005, Erratum: An ultrasensitive nucleic acid biosensor based on the catalytic oxidation of guanine by a novel redox threading intercalator (Chemical Communication (2005) DOI: 10.1039/b411803k), Chemical Communications, ISSN: 1359-7345
Tansil NC, Xie F, Xie H, et al., 2005, An ultrasensitive nucleic acid biosensor based on the catalytic oxidation of guanine by a novel redox threading intercalator, Chem. Commun., Vol: 0, Pages: 1064-1066-1064-1066
An ultrasensitive nucleic acid biosensor for the direct detection of attomoles nucleic acid in 1.0 [small micro]l droplets is described which can be used for detection of cancer marker genes in mRNA extracted from human breast tissues without a RT-PCR step.
Xie F, Goldys E, 2005, Homogeneous Au-Core Ag-Shell Nanoparticles Preparation for Fluorescence Enhancement Application, A Simple Method to Prepare Homogeneous SilverNanoparticles on Glass Substrate and Their Application for Fluorescence Enhancement
Le VD, Xie F, Yang Z, et al., 2005, An ultrasensitive protein array based on electrochemical enzyme immunoassay, Front. Biosci., Vol: 10, Pages: 1654-1660-1654-1660
An ultrasensitive electrochemical enzyme immunoassay (EEIA) for the detection of proteins on an 8x8 array is described. The assay is based on wired enzyme technology. Briefly, capture antibody was covalently immobilized on a self-assembled 11-mercaptoundecanoic acid (MUA) monolayer coated gold electrode. After incubating with a target protein (antigen), the gold electrode was treated sequentially in biotinylated detection antibody solution and in avidin-horseradish peroxidase conjugate (A-HRP) solution. A cationic redox polymer (electrochemical mediator) overcoating was applied to the gold electrode through layer-by-layer electrostatic self-assembly. The formation of a bilayer brought the HRP in electrical contact with the underlying electrode, making the bilayer an electrocatalyst for the reduction of hydrogen peroxide where the redox polymer acts as an artificial mediator. Consequently, the concentration of protein could be quantified amperometrically. This electrochemical immunoassay combined the specificity of the immunological reaction with the sensitivity of the electrochemical detection. The applicability of the system in protein detection was demonstrated with a snake toxin, beta-bungarotoxin, a neurotoxin from the venom of the snake Bungarus multicinctues. Under optimized experimental conditions, the assay allowed the detection of beta-bungarotoxin in the range of 20 pg/mL to 1.5 ng/mL with a detection limit of 10 pg/mL (20 fg). A higher detection limit of 25 pg/mL was obtained in serum.
Tansil NC, Xie H, Xie F, et al., 2004, Direct Detection of DNA with an Electrocatalytic Threading Intercalator, Analytical Chemistry, Vol: 77, Pages: 126-134, ISSN: 0003-2700
Xie H, Yu YH, Xie F, et al., 2004, A Nucleic Acid Biosensor for Gene Expression Analysis in Nanograms of mRNA, Analytical Chemistry, Vol: 76, Pages: 4023-4029, ISSN: 0003-2700
An ultrasensitive nucleic acid biosensor for direct detection of genes in mRNA extracted from animal tissues is described. It is based on amperometric detection of a target gene by forming an mRNA/redox polymer bilayer on a gold electrode. The mRNA was directly labeled with cisplatin?biotin conjugates through coordinative bonds with purine bases in the mRNA molecules. A subsequent binding of glucose oxidase?avidin conjugates to the labeled mRNA and the introduction of a poly(vinylimidazole-co-acrylamide) partially imidazole-complexed with [Os(bpy)2(im)] (bpy = 2,2?-bipyridine, im = imidazole) redox polymer overcoating to the electrode allowed for electrochemical detection of the oxidation current of glucose in solution. Depending on individual genes, detection limits of subfemtograms were achieved. As compared to a sandwich-type assay, the sensitivity was improved by as much as 25-fold through the incorporation of multiple enzyme labels to the mRNA molecules. Less than 2-fold gene expression difference was unambiguously differentiated in as little as 5.0 ng of mRNA. With the greatly improved sensitivity, at least 1000-fold more sensitive than fluorescence-based techniques, the amount of mRNA needed in the assay was cut down from microgram to nanogram levels.
Xie H, Yu Y, Xie F, et al., 2004, Breast Cancer Susceptibility Gene mRNAs Quantified by Microarrays with Electrochemical Detection, Clinical Chemistry, Vol: 50, Pages: 1231-1233, ISSN: 0009-9147
Gao Z, Xie F, Arshad MSM, et al., 2004, A disposable glucose biosensor based on diffusional mediator dispersed in nanoparticulate membrane on screen-printed carbon electrode, Nanotechnology Conference and Trade Show (Nanotech 2004), Publisher: NANO SCIENCE & TECHNOLOGY INST, Pages: 209-212
Xie H, Xie F, Tansil N, et al., 2004, An Ultrasensitive Nucleic Acid Biosensor for Gene Expression Analysis in mRNA, International SBE Conference on Bioengineering and Nanotechnology
Xie F, Xie H, Tansil N, et al., 2004, Direct Amperometric Detection of DNA by Electrocatalysis of Ascorbic Acid with an Electrocatalytic Threading Intercalator, International SBE Conference on Bioengineering and Nanotechnology
Xie H, Xie F, Gao Z, 2004, An Electrochemical Biosensor Array for Breast Cancer Gene Detection, Eurosensors XVIII
Gao Z, Xie F, Arshad M, et al., 2004, A Glucose Biosensor with a Nanoparticlate Regulating Membrane, Proceedings of Nanotech 2004, Boston, Massachusetts
Zeng HC, Xie F, Wong KC, et al., 2002, Insertion and Removal of Protons in Single-Crystal Orthorhombic Molybdenum Trioxide under HS/Hand O/N, Chemistry of Materials, Vol: 14, Pages: 1788-1796, ISSN: 0897-4756
Zeng HC, Ng WK, Cheong LH, et al., 2001, Insertion Direction of Hydrogen in Protonation of α-MoO3, The Journal of Physical Chemistry B, Vol: 105, Pages: 7178-7181, ISSN: 1520-6106
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