Imperial College London

DrGeoffBaldwin

Faculty of Natural SciencesDepartment of Life Sciences

Reader in Biochemistry
 
 
 
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Contact

 

+44 (0)20 7594 5288g.baldwin

 
 
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Location

 

508Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Publication Type
Year
to

47 results found

Girvan P, Teng X, Brooks NJ, Baldwin GS, Ying Let al., 2019, Redox Kinetics of the Amyloid-Beta-Copper Complex and Its Biological Implications, 63rd Annual Meeting of the Biophysical-Society, Publisher: CELL PRESS, Pages: 28A-28A, ISSN: 0006-3495

CONFERENCE PAPER

Silhan J, Zhao Q, Boura E, Thomson H, Forster A, Tang CM, Freemont PS, Baldwin GSet al., 2018, Structural basis for recognition and repair of the 3 '-phosphate by NExo, a base excision DNA repair nuclease from Neisseria meningitidis, NUCLEIC ACIDS RESEARCH, Vol: 46, Pages: 11980-11989, ISSN: 0305-1048

JOURNAL ARTICLE

Girvan P, Teng X, Brooks NJ, Baldwin GS, Ying Let al., 2018, Redox Kinetics of the Amyloid-beta-Cu Complex and Its Biological Implications, BIOCHEMISTRY, Vol: 57, Pages: 6228-6233, ISSN: 0006-2960

JOURNAL ARTICLE

Beal J, Haddock-Angelli T, Baldwin G, Gershater M, Dwijayanti A, Storch M, de Mora K, Lizarazo M, Rettberg Ret al., 2018, Quantification of bacterial fluorescence using independent calibrants, PLOS ONE, Vol: 13, ISSN: 1932-6203

JOURNAL ARTICLE

Storch M, Casini A, Mackrow B, Ellis T, Baldwin GSet al., 2017, BASIC: A Simple and Accurate Modular DNA Assembly Method., Methods Mol Biol, Vol: 1472, Pages: 79-91

Biopart Assembly Standard for Idempotent Cloning (BASIC) is a simple, accurate, and robust DNA assembly method. The method is based on linker-mediated DNA assembly and provides highly accurate DNA assembly with 99 % correct assemblies for four parts and 90 % correct assemblies for seven parts [1]. The BASIC standard defines a single entry vector for all parts flanked by the same prefix and suffix sequences and its idempotent nature means that the assembled construct is returned in the same format. Once a part has been adapted into the BASIC format it can be placed at any position within a BASIC assembly without the need for reformatting. This allows laboratories to grow comprehensive and universal part libraries and to share them efficiently. The modularity within the BASIC framework is further extended by the possibility of encoding ribosomal binding sites (RBS) and peptide linker sequences directly on the linkers used for assembly. This makes BASIC a highly versatile library construction method for combinatorial part assembly including the construction of promoter, RBS, gene variant, and protein-tag libraries. In comparison with other DNA assembly standards and methods, BASIC offers a simple robust protocol; it relies on a single entry vector, provides for easy hierarchical assembly, and is highly accurate for up to seven parts per assembly round [2].

JOURNAL ARTICLE

Webb AJ, Kelwick R, Doenhoff MJ, Kylilis N, MacDonald JT, Wen KY, McKeown C, Baldwin G, Ellis T, Jensen K, Freemont PSet al., 2016, A protease-based biosensor for the detection of schistosome cercariae, SCIENTIFIC REPORTS, Vol: 6, ISSN: 2045-2322

JOURNAL ARTICLE

Casini A, Storch M, Baldwin GS, Ellis Tet al., 2015, Bricks and blueprints: methods and standards for DNA assembly, NATURE REVIEWS MOLECULAR CELL BIOLOGY, Vol: 16, Pages: 568-576, ISSN: 1471-0072

JOURNAL ARTICLE

Storch M, Casini A, Mackrow B, Fleming T, Trewhitt H, Ellis T, Baldwin GSet al., 2015, BASIC: A New Biopart Assembly Standard for Idempotent Cloning Provides Accurate, Single-Tier DNA Assembly for Synthetic Biology, ACS SYNTHETIC BIOLOGY, Vol: 4, Pages: 781-787, ISSN: 2161-5063

JOURNAL ARTICLE

Robinson T, Valluri P, Kennedy G, Sardini A, Dunsby C, Neil MAA, Baldwin GS, French PMW, de Mello AJet al., 2014, Analysis of DNA Binding and Nucleotide Flipping Kinetics Using Two-Color Two-Photon Fluorescence Lifetime Imaging Microscopy, ANALYTICAL CHEMISTRY, Vol: 86, Pages: 10732-10740, ISSN: 0003-2700

JOURNAL ARTICLE

Casini A, Christodoulou G, Freemont PS, Baldwin GS, Ellis T, MacDonald JTet al., 2014, R2oDNA Designer: Computational Design of Biologically Neutral Synthetic DNA Sequences, ACS SYNTHETIC BIOLOGY, Vol: 3, Pages: 525-528, ISSN: 2161-5063

JOURNAL ARTICLE

Casini A, MacDonald JT, De Jonghe J, Christodoulou G, Freemont PS, Baldwin GS, Ellis Tet al., 2014, One-pot DNA construction for synthetic biology: the Modular Overlap-Directed Assembly with Linkers (MODAL) strategy, NUCLEIC ACIDS RESEARCH, Vol: 42, ISSN: 0305-1048

JOURNAL ARTICLE

Cehovin A, Simpson PJ, McDowell MA, Brown DR, Noschese R, Pallett M, Brady J, Baldwin GS, Lea SM, Matthews SJ, Pelicic Vet al., 2013, Specific DNA recognition mediated by a type IV pilin, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 110, Pages: 3065-3070, ISSN: 0027-8424

JOURNAL ARTICLE

Lu D, Silhan J, MacDonald JT, Carpenter EP, Jensen K, Tang CM, Baldwin GS, Freemont PSet al., 2012, Structural basis for the recognition and cleavage of abasic DNA in Neisseria meningitidis, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 109, Pages: 16852-16857, ISSN: 0027-8424

JOURNAL ARTICLE

Silhan J, Nagorska K, Zhao Q, Jensen K, Freemont PS, Tang CM, Baldwin GSet al., 2012, Specialization of an Exonuclease III family enzyme in the repair of 3' DNA lesions during base excision repair in the human pathogen Neisseria meningitidis, NUCLEIC ACIDS RESEARCH, Vol: 40, Pages: 2065-2075, ISSN: 0305-1048

JOURNAL ARTICLE

Nagorska K, Silhan J, Li Y, Pelicic V, Freemont PS, Baldwin GS, Tang CMet al., 2012, A network of enzymes involved in repair of oxidative DNA damage in Neisseria meningitidis, MOLECULAR MICROBIOLOGY, Vol: 83, Pages: 1064-1079, ISSN: 0950-382X

JOURNAL ARTICLE

Baldwin G, Bayer T, Dickinson R, Ellis T, Freemont PS, Kitney RI, Polizzi K, Stan GBet al., 2012, Synthetic Biology - A Primer, ISBN: 9781848168626

© 2012 by Imperial College Press. All rights reserved. Synthetic Biology — A Primer gives a broad overview of the emerging field of synthetic biology and the foundational concepts on which it is built. It will be of interest to final year undergraduates, postgraduates and established researchers who are interested in learning about this exciting new field. The book introduces readers to fundamental concepts in molecular biology and engineering and then explores the two major themes for synthetic biology, namely ‘bottom-up’ and ‘top-down’ engineering approaches. ‘Top-down’ engineering utilises a conceptual framework of engineering and systematic design to build new biological systems by integrating robustly characterised biological parts into an existing system through the use of extensive mathematical modelling. The ‘bottom-up’ approach involves the design and building of synthetic protocells using basic chemical and biochemical building blocks from scratch. Exemplars of cutting-edge applications designed using synthetic biology principles are presented, including the production of novel biofuels from renewable feedstocks, microbial synthesis of pharmaceuticals and fine chemicals, and the design and implementation of biosensors to detect infections and environmental waste. The book also uses the Internationally Genetically Engineered Machine (iGEM) competition to illustrate the power of synthetic biology as an innovative research and training science. Finally, the primer includes a chapter on the ethical, legal and societal issues surrounding synthetic biology, illustrating the integration of social sciences in synthetic biology research.

BOOK

Sheppard C, Camara B, Shadrin A, Akulenko N, Lu M, Baldwin G, Severinov K, Cota E, Matthews S, Wigneshweraraj SRet al., 2011, Inhibition of Escherichia coli RNAp by T7 Gp2 Protein: Role of Negatively Charged Strip of Amino Acid Residues in Gp2, JOURNAL OF MOLECULAR BIOLOGY, Vol: 407, Pages: 623-632, ISSN: 0022-2836

JOURNAL ARTICLE

Grippon S, Zhao Q, Robinson T, Marshall JJT, O'Neill RJ, Manning H, Kennedy G, Dunsby C, Neil M, Halford SE, French PMW, Baldwin GSet al., 2011, Differential modes of DNA binding by mismatch uracil DNA glycosylase from Escherichia coli: implications for abasic lesion processing and enzyme communication in the base excision repair pathway, NUCLEIC ACIDS RESEARCH, Vol: 39, Pages: 2593-2603, ISSN: 0305-1048

JOURNAL ARTICLE

Ivanov AP, Instuli E, McGilvery CM, Baldwin G, McComb DW, Albrecht T, Edel JBet al., 2011, DNA Tunneling Detector Embedded in a Nanopore, NANO LETTERS, Vol: 11, Pages: 279-285, ISSN: 1530-6984

JOURNAL ARTICLE

Ellis T, Adie T, Baldwin GS, 2011, DNA assembly for synthetic biology: from parts to pathways and beyond, INTEGRATIVE BIOLOGY, Vol: 3, Pages: 109-118, ISSN: 1757-9694

JOURNAL ARTICLE

Ayub M, Ivanov A, Instuli E, Cecchini M, Chansin G, McGilvery C, Hong J, Baldwin G, McComb D, Edel JB, Albrecht Tet al., 2010, Nanopore/electrode structures for single-molecule biosensing, ELECTROCHIMICA ACTA, Vol: 55, Pages: 8237-8243, ISSN: 0013-4686

JOURNAL ARTICLE

Robinson T, Manning HB, Dunsby C, Neil MAA, Baldwin GS, de Mello AJ, French PMWet al., 2010, Investigating fast enzyme-DNA kinetics using multidimensional fluorescence imaging and microfluidics, Conference on Microfluidics, BioMEMS, and Medical Microsystems VIII, Publisher: SPIE-INT SOC OPTICAL ENGINEERING, ISSN: 0277-786X

CONFERENCE PAPER

Baldwin GS, Brooks NJ, Robson RE, Wynveen A, Goldar A, Leikin S, Seddon JM, Kornyshev AAet al., 2009, DNA Double Helices Recognize Mutual Sequence Homology in a Protein Free Environment, JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS, Vol: 26, Pages: 880-880, ISSN: 0739-1102

JOURNAL ARTICLE

Robinson T, Schaerli Y, Wootton R, Hollfelder F, Dunsby C, Baldwin G, Neil M, French P, deMello Aet al., 2009, Removal of background signals from fluorescence thermometry measurements in PDMS microchannels using fluorescence lifetime imaging, LAB ON A CHIP, Vol: 9, Pages: 3437-3441, ISSN: 1473-0197

JOURNAL ARTICLE

Robinson T, Valluri P, Manning HB, Owen DM, Munro I, Talbot CB, Dunsby C, Eccleston JF, Baldwin GS, Neil MAA, de Mello AJ, French PMWet al., 2008, Three-dimensional molecular mapping in a microfluidic mixing device using fluorescence lifetime imaging, OPTICS LETTERS, Vol: 33, Pages: 1887-1889, ISSN: 0146-9592

JOURNAL ARTICLE

Briggs LC, Baldwin GS, Miyata N, Kondo H, Zhang X, Freemont PSet al., 2008, Analysis of nucleotide binding to p97 reveals the properties of a tandem AAA hexameric ATPase, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 283, Pages: 13745-13752, ISSN: 0021-9258

JOURNAL ARTICLE

Baldwin GS, Brooks NJ, Robson RE, Wynveen A, Goldar A, Leikin S, Seddon JM, Kornyshev AAet al., 2008, DNA double helices recognize mutual sequence homology in a protein free environment, JOURNAL OF PHYSICAL CHEMISTRY B, Vol: 112, Pages: 1060-1064, ISSN: 1520-6106

JOURNAL ARTICLE

Di Noia JM, Williams GT, Chan DTY, Buerstedde J-M, Baldwin GS, Neuberger MSet al., 2007, Dependence of antibody gene diversification on uracil excision, JOURNAL OF EXPERIMENTAL MEDICINE, Vol: 204, Pages: 3209-3219, ISSN: 0022-1007

JOURNAL ARTICLE

Carpenter EP, Corbett A, Thomson H, Adacha J, Jensen K, Bergeron J, Kasampalidis I, Exley R, Winterbotham M, Tang C, Baldwin GS, Freemont Pet al., 2007, AP endonuclease paralogues with distinct activities in DNA repair and bacterial pathogenesis, EMBO JOURNAL, Vol: 26, Pages: 1363-1372, ISSN: 0261-4189

JOURNAL ARTICLE

Bellamy SRW, Krusong K, Baldwin GS, 2007, A rapid reaction analysis of uracil DNA glycosylase indicates an active mechanism of base flipping, NUCLEIC ACIDS RESEARCH, Vol: 35, Pages: 1478-1487, ISSN: 0305-1048

JOURNAL ARTICLE

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