Imperial College London


Faculty of Natural SciencesDepartment of Life Sciences

Reader in Biochemistry



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BibTex format

author = {Casini, A and Storch, M and Baldwin, GS and Ellis, T},
doi = {10.1038/nrm4014},
journal = {Nature Reviews Molecular Cell Biology},
pages = {568--576},
title = {Bricks and blueprints: methods and standards for DNA assembly},
url = {},
volume = {16},
year = {2015}

RIS format (EndNote, RefMan)

AB - DNA assembly is a key part of constructing gene expression systems and even whole chromosomes. In the past decade, a plethora of powerful new DNA assembly methods — including Gibson Assembly, Golden Gate and ligase cycling reaction (LCR) — have been developed. In this Innovation article, we discuss these methods as well as standards such as the modular cloning (MoClo) system, GoldenBraid, modular overlap-directed assembly with linkers (MODAL) and PaperClip, which have been developed to facilitate a streamlined assembly workflow, to aid the exchange of material between research groups and to create modular reusable DNA parts.
AU - Casini,A
AU - Storch,M
AU - Baldwin,GS
AU - Ellis,T
DO - 10.1038/nrm4014
EP - 576
PY - 2015///
SN - 1471-0080
SP - 568
TI - Bricks and blueprints: methods and standards for DNA assembly
T2 - Nature Reviews Molecular Cell Biology
UR -
UR -
UR -
VL - 16
ER -