225 results found
Stevens MP, Frankel GM, 2014, The Locus of Enterocyte Effacement and Associated Virulence Factors of Enterohemorrhagic Escherichia coli., Microbiol Spectr, Vol: 2
A subset of Shiga toxin-producing Escherichia coli strains, termed enterohemorrhagic E. coli (EHEC), is defined in part by the ability to produce attaching and effacing (A/E) lesions on intestinal epithelia. Such lesions are characterized by intimate bacterial attachment to the apical surface of enterocytes, cytoskeletal rearrangements beneath adherent bacteria, and destruction of proximal microvilli. A/E lesion formation requires the locus of enterocyte effacement (LEE), which encodes a Type III secretion system that injects bacterial proteins into host cells. The translocated proteins, termed effectors, subvert a plethora of cellular pathways to the benefit of the pathogen, for example, by recruiting cytoskeletal proteins, disrupting epithelial barrier integrity, and interfering with the induction of inflammation, phagocytosis, and apoptosis. The LEE and selected effectors play pivotal roles in intestinal persistence and virulence of EHEC, and it is becoming clear that effectors may act in redundant, synergistic, and antagonistic ways during infection. Vaccines that target the function of the Type III secretion system limit colonization of reservoir hosts by EHEC and may thus aid control of zoonotic infections. Here we review the features and functions of the LEE-encoded Type III secretion system and associated effectors of E. coli O157:H7 and other Shiga toxin-producing E. coli strains.
Wlodarska M, Thaiss CA, Nowarski R, et al., 2014, NLRP6 Inflammasome Orchestrates the Colonic Host-Microbial Interface by Regulating Goblet Cell Mucus Secretion, CELL, Vol: 156, Pages: 1045-1059, ISSN: 0092-8674
Young JC, Clements A, Lang AE, et al., 2014, The Escherichia coli effector EspJ blocks Src kinase activity via amidation and ADP ribosylation, NATURE COMMUNICATIONS, Vol: 5, ISSN: 2041-1723
Aurass P, Schlegel M, Metwally O, et al., 2013, The Legionella pneumophila Dot/Icm-secreted Effector PlcC/CegC1 Together with PlcA and PlcB Promotes Virulence and Belongs to a Novel Zinc Metallophospholipase C Family Present in Bacteria and Fungi, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 288, Pages: 11080-11092, ISSN: 0021-9258
Clare S, John V, Walker AW, et al., 2013, Enhanced Susceptibility to Citrobacter rodentium Infection in MicroRNA-155-Deficient Mice, INFECTION AND IMMUNITY, Vol: 81, Pages: 723-732, ISSN: 0019-9567
© 2015 Elsevier Inc. All rights reserved. Enterohemorrhagic E. coli (EHEC), enteropathogenic E. coli (EPEC), and Shigella use a type 3 secretion system (T3SS) to inject dozens of effector proteins into the host cell. The effectors manipulate host cell processes including the host cytoskeleton, immune response, cell survival, and gut integrity. EPEC and EHEC share a large number of common effectors and some homologous effectors can be found in Shigella, however there is also considerable variability within and between pathotypes. For many effectors detailed molecular mechanisms of action have been described by identifying host interacting partners, homologous proteins, or enzymatic activities. However, understanding effector biology within the context of infection with multiple effectors, various cell populations, and host genetic differences remains a challenge.
Collins JW, Meganck JA, Kuo C, et al., 2013, 4D Multimodality Imaging of Citrobacter rodentium Infections in Mice, JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, ISSN: 1940-087X
Harding CR, Mattheis C, Mousnier A, et al., 2013, LtpD Is a Novel Legionella pneumophila Effector That Binds Phosphatidylinositol 3-Phosphate and Inositol Monophosphatase IMPA1, INFECTION AND IMMUNITY, Vol: 81, Pages: 4261-4270, ISSN: 0019-9567
Harding CR, Schroeder GN, Collins JW, et al., 2013, Use of Galleria mellonella as a Model Organism to Study Legionella pneumophila Infection, JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, ISSN: 1940-087X
Harding CR, Stoneham CA, Schuelein R, et al., 2013, The Dot/Icm Effector SdhA Is Necessary for Virulence of Legionella pneumophila in Galleria mellonella and A/J Mice, INFECTION AND IMMUNITY, Vol: 81, Pages: 2598-2605, ISSN: 0019-9567
Lai Y, Rosenshine I, Leong JM, et al., 2013, Intimate host attachment: enteropathogenic and enterohaemorrhagic Escherichia coli, CELLULAR MICROBIOLOGY, Vol: 15, Pages: 1796-1808, ISSN: 1462-5814
Pearson JS, Giogha C, Ong SY, et al., 2013, A type III effector antagonizes death receptor signalling during bacterial gut infection, NATURE, Vol: 501, Pages: 247-+, ISSN: 0028-0836
Raymond B, Young JC, Pallett M, et al., 2013, Subversion of trafficking, apoptosis, and innate immunity by type III secretion system effectors, TRENDS IN MICROBIOLOGY, Vol: 21, Pages: 430-439, ISSN: 0966-842X
Vogrin AJ, Mousnier A, Frankel G, et al., 2013, Subcellular localization of legionella Dot/Icm effectors., Methods Mol Biol, Vol: 954, Pages: 333-344
The translocation of effector proteins by the Dot/Icm type IV secretion system is central to the ability of Legionella pneumophila to persist and replicate within eukaryotic cells. The subcellular localization of translocated Dot/Icm proteins in host cells provides insight into their function. Through co-staining with host cell markers, effector proteins may be localized to specific subcellular compartments and membranes, which frequently reflects their host cell target and mechanism of action. In this chapter, we describe protocols to (1) localize effector proteins within cells by ectopic expression using green fluorescent protein fusions and (2) localize effector proteins within infected cells using epitope-tagged effector proteins and immuno-fluorescence microscopy.
Berger CN, Crepin VF, Baruch K, et al., 2012, EspZ of Enteropathogenic and Enterohemorrhagic Escherichia coli Regulates Type III Secretion System Protein Translocation, MBIO, Vol: 3, ISSN: 2150-7511
Clements A, Young JC, Constantinou N, et al., 2012, Infection strategies of enteric pathogenic Escherichia coli., Gut Microbes, Vol: 3, Pages: 71-87
Enteric Escherichia coli (E. coli) are both natural flora of humans and important pathogens causing significant morbidity and mortality worldwide. Traditionally enteric E. coli have been divided into 6 pathotypes, with further pathotypes often proposed. In this review we suggest expansion of the enteric E. coli into 8 pathotypes to include the emerging pathotypes of adherent invasive E. coli (AIEC) and Shiga-toxin producing enteroaggregative E. coli (STEAEC). The molecular mechanisms that allow enteric E. coli to colonize and cause disease in the human host are examined and for two of the pathotypes that express a type 3 secretion system (T3SS) we discuss the complex interplay between translocated effectors and manipulation of host cell signaling pathways that occurs during infection.
Collins JW, Akin AR, Kosta A, et al., 2012, Pre-treatment with Bifidobacterium breve UCC2003 modulates Citrobacter rodentium-induced colonic inflammation and organ specificity, MICROBIOLOGY-SGM, Vol: 158, Pages: 2826-2834, ISSN: 1350-0872
Dart AE, Tollis S, Bright MD, et al., 2012, The motor protein myosin 1G functions in Fc gamma R-mediated phagocytosis, JOURNAL OF CELL SCIENCE, Vol: 125, Pages: 6020-6029, ISSN: 0021-9533
Dolezal P, Aili M, Tong J, et al., 2012, Legionella pneumophila Secretes a Mitochondrial Carrier Protein during Infection, PLOS PATHOGENS, Vol: 8, ISSN: 1553-7366
Harding CR, Schroeder GN, Reynolds S, et al., 2012, Legionella pneumophila Pathogenesis in the Galleria mellonella Infection Model, INFECTION AND IMMUNITY, Vol: 80, Pages: 2780-2790, ISSN: 0019-9567
Islam MS, Shaw RK, Frankel G, et al., 2012, Translation of a minigene in the 5 ' leader sequence of the enterohaemorrhagic Escherichia coli LEE1 transcription unit affects expression of the neighbouring downstream gene, BIOCHEMICAL JOURNAL, Vol: 441, Pages: 247-253, ISSN: 0264-6021
Munera D, Martinez E, Varyukhina S, et al., 2012, Recruitment and membrane interactions of host cell proteins during attachment of enteropathogenic and enterohaemorrhagic Escherichia coli, BIOCHEMICAL JOURNAL, Vol: 445, Pages: 383-392, ISSN: 0264-6021
Nesta B, Spraggon G, Alteri C, et al., 2012, FdeC, a Novel Broadly Conserved Escherichia coli Adhesin Eliciting Protection against Urinary Tract Infections, MBIO, Vol: 3, ISSN: 2150-7511
Wong ARC, Clements A, Raymond B, et al., 2012, The Interplay between the Escherichia coli Rho Guanine Nucleotide Exchange Factor Effectors and the Mammalian RhoGEF Inhibitor EspH, MBIO, Vol: 3, ISSN: 2150-7511
Wong ARC, Raymond B, Collins JW, et al., 2012, The enteropathogenic E. coli effector EspH promotes actin pedestal formation and elongation via WASP-interacting protein (WIP), CELLULAR MICROBIOLOGY, Vol: 14, Pages: 1051-1070, ISSN: 1462-5814
Arbeloa A, Oates CV, Marches O, et al., 2011, Enteropathogenic and Enterohemorrhagic Escherichia coli Type III Secretion Effector EspV Induces Radical Morphological Changes in Eukaryotic Cells, INFECTION AND IMMUNITY, Vol: 79, Pages: 1067-1076, ISSN: 0019-9567
Berger CN, Brown DJ, Shaw RK, et al., 2011, Salmonella enterica strains belonging to O serogroup 1,3,19 induce chlorosis and wilting of Arabidopsis thaliana leaves, ENVIRONMENTAL MICROBIOLOGY, Vol: 13, Pages: 1299-1308, ISSN: 1462-2912
Bright MD, Frankel G, 2011, PAK4 phosphorylates myosin regulatory light chain and contributes to Fc gamma receptor-mediated phagocytosis, INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, Vol: 43, Pages: 1776-1781, ISSN: 1357-2725
Clements A, Smollett K, Lee SF, et al., 2011, EspG of enteropathogenic and enterohemorrhagic E. coli binds the Golgi matrix protein GM130 and disrupts the Golgi structure and function, CELLULAR MICROBIOLOGY, Vol: 13, Pages: 1429-1439, ISSN: 1462-5814
Fookes M, Schroeder GN, Langridge GC, et al., 2011, Salmonella bongori Provides Insights into the Evolution of the Salmonellae, PLOS PATHOGENS, Vol: 7, ISSN: 1553-7366
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