Imperial College London

ProfessorGadFrankel

Faculty of Natural SciencesDepartment of Life Sciences

Professor of Molecular Pathogenesis
 
 
 
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Contact

 

+44 (0)20 7594 5253g.frankel

 
 
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Location

 

1.46Flowers buildingSouth Kensington Campus

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Summary

 

Publications

Publication Type
Year
to

223 results found

Girard F, Dziva F, Stevens MP, Frankel Get al., 2009, Interactions of Typical and Atypical Enteropathogenic Escherichia coli Strains with the Calf Intestinal Mucosa Ex Vivo, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Vol: 75, Pages: 5991-5995, ISSN: 0099-2240

JOURNAL ARTICLE

Griesenbach U, Meng C, Farley R, Gardner A, Brake MA, Frankel GM, Gruenert DC, Cheng SH, Scheule RK, Alton EWFWet al., 2009, The role of doxorubicin in non-viral gene transfer in the lung, BIOMATERIALS, Vol: 30, Pages: 1971-1977, ISSN: 0142-9612

JOURNAL ARTICLE

Iguchi A, Thomson NR, Ogura Y, Saunders D, Ooka T, Henderson IR, Harris D, Asadulghani M, Kurokawa K, Dean P, Kenny B, Quail MA, Thurston S, Dougan G, Hayashi T, Parkhill J, Frankel Get al., 2009, Complete Genome Sequence and Comparative Genome Analysis of Enteropathogenic Escherichia coli O127:H6 Strain E2348/69, JOURNAL OF BACTERIOLOGY, Vol: 191, Pages: 347-354, ISSN: 0021-9193

JOURNAL ARTICLE

Wiles S, Robertson BD, Frankel G, Kerton Aet al., 2009, Bioluminescent monitoring of in vivo colonization and clearance dynamics by light-emitting bacteria., Methods Mol Biol, Vol: 574, Pages: 137-153

Bioluminescence is an excellent reporter system for analysing bacterial colonization and clearance dynamics in vivo. Many bacterial species have been rendered bioluminescent, allowing the sensitive detection of bacterial burden and metabolic activity in real-time and in situ in living animals. In this chapter we describe the protocols for characterizing in vivo infection models using bioluminescent bacteria: from real-time imaging in living animals by bioluminescence imaging (BLI) to ex vivo BLI of harvested organs and tissues and, finally, to quantification of bacterial numbers in organ and tissue homogenates by luminometry and viable counts. While the lux operon from Photorhabdus luminescens is ideally suited for use in such models, there may be times when alternative luciferases, such as those from the firefly (luc) or marine copepods (Gluc), may be more appropriate. Here we describe the protocols required to monitor colonization and clearance dynamics using bioluminescent bacteria that are lux-, luc-, or Gluc-positive.

JOURNAL ARTICLE

Arbeloa A, Bulgin RR, MacKenzie G, Shaw RK, Pallen MJ, Crepin VF, Berger CN, Frankel Get al., 2008, Subversion of actin dynamics by EspM effectors of attaching and effacing bacterial pathogens, CELLULAR MICROBIOLOGY, Vol: 10, Pages: 1429-1441, ISSN: 1462-5814

JOURNAL ARTICLE

Bai L, Schuller S, Whale A, Mousnier A, Marches O, Wang L, Ooka T, Heuschkel R, Torrente F, Kaper JB, Gomes TAT, Xu J, Phillips AD, Frankel Get al., 2008, Enteropathogenic Escherichia coli O125 : H6 triggers attaching and effacing lesions on human intestinal biopsy specimens independently of nck and TccP/TccP2, INFECTION AND IMMUNITY, Vol: 76, Pages: 361-368, ISSN: 0019-9567

JOURNAL ARTICLE

Crepin VF, Martinez E, Shaw RK, Frankel G, Daniell SJet al., 2008, Structural and functional properties of chimeric EspA-FliCi filaments of EPEC, JOURNAL OF MOLECULAR BIOLOGY, Vol: 378, Pages: 243-250, ISSN: 0022-2836

JOURNAL ARTICLE

Dennis A, Kudo T, Kruidenier L, Girard F, Crepin VF, MacDonald TT, Frankel G, Wiles Set al., 2008, The p50 Subunit of NF-kappa B Is Critical for In Vivo Clearance of the Noninvasive Enteric Pathogen Citrobacter rodentium, INFECTION AND IMMUNITY, Vol: 76, Pages: 4978-4988, ISSN: 0019-9567

JOURNAL ARTICLE

Frankel G, Phillips AD, 2008, Attaching effacing Escherichia coli and paradigms of Tir-triggered actin polymerization: getting off the pedestal, CELLULAR MICROBIOLOGY, Vol: 10, Pages: 549-556, ISSN: 1462-5814

JOURNAL ARTICLE

Girard F, Frankel G, Phillips AD, Cooley W, Weyer U, Dugdale AHA, Woodward MJ, La Ragione RMet al., 2008, Interaction of enterohemorrhagic Escherichia coli O157 : H7 with mouse intestinal mucosa, FEMS MICROBIOLOGY LETTERS, Vol: 283, Pages: 196-202, ISSN: 0378-1097

JOURNAL ARTICLE

Griesenbach U, Meng C, Farley R, Cheng SH, Scheule RK, Davies MH, Wolstenholme-Hogg PC, ten Hove W, van der Hoeven P, Sinn PL, McCray PB, Inoue M, Geddes DM, Hasegawa M, Frankel G, Wiles S, Alton EWFWet al., 2008, In vivo imaging of gene transfer to the respiratory tract, BIOMATERIALS, Vol: 29, Pages: 1533-1540, ISSN: 0142-9612

JOURNAL ARTICLE

Hart E, Yang J, Tauschek M, Kelly M, Wakefield MJ, Frankel G, Hartland EL, Robins-Browne RMet al., 2008, RegA, an AraC-Like Protein, Is a Global Transcriptional Regulator That Controls Virulence Gene Expression in Citrobacter rodentium, INFECTION AND IMMUNITY, Vol: 76, Pages: 5247-5256, ISSN: 0019-9567

JOURNAL ARTICLE

Hemrajani C, Marches O, Wiles S, Girard F, Dennis A, Dziva F, Best A, Phillips AD, Berger CN, Mousnier A, Crepin VF, Kruidenier L, Woodward MJ, Stevens MP, La Ragione RM, MacDonald TT, Frankel Get al., 2008, Role of NleH, a Type III Secreted Effector from Attaching and Effacing Pathogens, in Colonization of the Bovine, Ovine, and Murine Gut, INFECTION AND IMMUNITY, Vol: 76, Pages: 4804-4813, ISSN: 0019-9567

JOURNAL ARTICLE

Iguchi A, Ooka T, Ogura Y, Nakayama K, Frankel G, Hayashi Tet al., 2008, Genomic comparison of the O-antigen biosynthesis gene clusters of Escherichia coli O55 strains belonging to three distinct lineages, MICROBIOLOGY-SGM, Vol: 154, Pages: 559-570, ISSN: 1350-0872

JOURNAL ARTICLE

Jenkins C, Evans J, Chart H, Willshaw GA, Frankel Get al., 2008, Escherichia coli serogroup O26 - a new look at an old adversary, JOURNAL OF APPLIED MICROBIOLOGY, Vol: 104, Pages: 14-25, ISSN: 1364-5072

JOURNAL ARTICLE

Kozub-Witkowski E, Krause G, Frankel G, Kramer D, Appel B, Beutin Let al., 2008, Serotypes and virutypes of enteropathogenic and enterohaemorrhagic Escherichia coli strains from stool samples of children with diarrhoea in Germany, JOURNAL OF APPLIED MICROBIOLOGY, Vol: 104, Pages: 403-410, ISSN: 1364-5072

JOURNAL ARTICLE

Lee SF, Kelly M, McAlister A, Luck SN, Garcia EL, Hall RA, Robins-Browne RM, Frankel G, Hartland ELet al., 2008, A C-terminal class IPDZ binding motif of EspI/NIeA modulates the virulence of attaching and effacing Escherichia coli and Citrobacter rodentium, CELL MICROBIOL, Vol: 10, Pages: 499-513, ISSN: 1462-5814

Enteropathogenic Escherichia coli induces characteristic attaching-effacing (A/E) lesions on the intestinal mucosa during infection. The locus of enterocyte effacement is essential for A/E lesion formation and encodes a type III secretion system that translocates multiple effector proteins into the host cell. Following translocation, EspI/NIeA localizes to the Golgi. Using the yeast two-hybrid system (Y2HS) and PSD-95/Disk-large/ZO-1 (PDZ)-domain protein array overlays, we identified 15 putative host-interacting partners of Espl. All but two of the target proteins contained PDZ domains. Examination of the EspI amino acid sequence revealed a C-terminal consensus class I PDZ binding motif. Deletion of the last 7 amino acids of EspI to generate EspI(Delta C7) abrogated the Y2HS interaction between EspI and 5 of the 6 putative host cell target proteins tested. Deletion of the EspI PDZ binding motif also resulted in delayed trafficking of EspI to the Golgi. Using a mouse model of infection, we showed that Citrobacter rodentium expressing truncated EspI(Delta C7) was attenuated when in competition with C. rodentium expressing full-length EspI. Overall, these results suggested that Espl may modulate the virulence of A/E pathogens by binding host PDZ-domain proteins.

JOURNAL ARTICLE

Lee SF, Kelly M, McAlister A, Luck SN, Garcia EL, Hall RA, Robins-Browne RM, Frankel G, Hartland ELet al., 2008, A C-terminal class IPDZ binding motif of EspI/NleA modulates the virulence of attaching and effacing Escherichia coli and Citrobacter rodentium, CELLULAR MICROBIOLOGY, Vol: 10, Pages: 499-513, ISSN: 1462-5814

JOURNAL ARTICLE

Marches O, Covarelli V, Dahan S, Cougoule C, Bhatta P, Frankel G, Caron Eet al., 2008, EspJ of enteropathogenic and enterohaemorrhagic Escherichia coli inhibits opsono-phagocytosis, CELLULAR MICROBIOLOGY, Vol: 10, Pages: 1104-1115, ISSN: 1462-5814

JOURNAL ARTICLE

Mousnier A, Whale AD, Schuller S, Leong JM, Phillips AD, Frankel Get al., 2008, Cortactin recruitment by enterohemorrhagic Escherichia coli O157 : H7 during infection in vitro and ex vivo, INFECTION AND IMMUNITY, Vol: 76, Pages: 4669-4676, ISSN: 0019-9567

JOURNAL ARTICLE

Shaw RK, Berger CN, Feys B, Knutton S, Pallen MJ, Frankel Get al., 2008, Enterohemorrhagic Escherichia coli exploits EspA filaments for attachment to salad leaves, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Vol: 74, Pages: 2908-2914, ISSN: 0099-2240

JOURNAL ARTICLE

Singh MP, Shaw RK, Knutton S, Pallen MJ, Crepin VF, Frankel Get al., 2008, Identification of amino acid residues within the N-terminal domain of EspA that play a role in EspA filament biogenesis and function, JOURNAL OF BACTERIOLOGY, Vol: 190, Pages: 2221-2226, ISSN: 0021-9193

JOURNAL ARTICLE

Bishop AL, Wiles S, Dougan G, Frankel Get al., 2007, Cell attachment properties and infectivity of host-adapted and environmentally adapted Citrobacter rodentium, MICROBES AND INFECTION, Vol: 9, Pages: 1316-1324, ISSN: 1286-4579

JOURNAL ARTICLE

Chong Y, Fitzhenry R, Heuschkel R, Torrente F, Frankel G, Phillips ADet al., 2007, Human intestinal tissue tropism in Escherichia coli O157 : H7 - initial colonization of terminal ileum and Peyer's patches and minimal colonic adhesion ex vivo, MICROBIOLOGY-SGM, Vol: 153, Pages: 794-802, ISSN: 1350-0872

JOURNAL ARTICLE

Dziva F, Vlisidou I, Crepin VF, Wallis TS, Frankel G, Stevens MPet al., 2007, Vaccination of calves with EspA, a key colonisation factor of Escherichia coli O157 : H7, induces antigen-specific humoral responses but does not confer protection against intestinal colonisation, VETERINARY MICROBIOLOGY, Vol: 123, Pages: 254-261, ISSN: 0378-1135

JOURNAL ARTICLE

Girard F, Dziva F, van Diemen P, Phillips AD, Stevens MP, Frankel Get al., 2007, Adherence of enterohemorrhagic Escherichia coli O157, O26, and O111 strains to bovine intestinal explants ex vivo, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Vol: 73, Pages: 3084-3090, ISSN: 0099-2240

JOURNAL ARTICLE

Mundy R, Petrovska L, Smollett K, Simpson N, Wilson RK, Yu J, Tu X, Rosenshine I, Clare S, Dougan G, Frankel Get al., 2007, Identification of a novel Citrobacter rodentium type III secreted protein, EspI, and roles of this and other secreted proteins in infection (vol 72, pg 2288, 2004), INFECTION AND IMMUNITY, Vol: 75, Pages: 3686-3686, ISSN: 0019-9567

JOURNAL ARTICLE

Mundy R, Schuller S, Girard F, Fairbrother JM, Phillips AD, Frankel Get al., 2007, Functional studies of intimin in vivo and ex vivo: implications for host specificity and tissue tropism, MICROBIOLOGY-SGM, Vol: 153, Pages: 959-967, ISSN: 1350-0872

JOURNAL ARTICLE

Ogura Y, Ooka T, Whale A, Garmendia J, Beutin L, Tennant S, Krause G, Morabito S, Chinen I, Tobe T, Abe H, Tozzoli R, Caprioli A, Rivas M, Robins-Browne R, Hayashi T, Frankel Get al., 2007, TccP2 of O157 : H7 and non-O157 enterohemorrhagic Escherichia coli (EHEC): Challenging the dogma of EHEC-induced actin polymerization, INFECTION AND IMMUNITY, Vol: 75, Pages: 604-612, ISSN: 0019-9567

JOURNAL ARTICLE

Ooka T, Vieira MAM, Ogura Y, Beutin L, La Ragione R, van Diemen PM, Stevens MP, Aktan I, Cawthraw S, Best A, Hernandes RT, Krause G, Gomes TAT, Hayashi T, Frankel Get al., 2007, Characterization of tccP2 carried by atypical enteropathogenic Escherichia coli, FEMS MICROBIOLOGY LETTERS, Vol: 271, Pages: 126-135, ISSN: 0378-1097

JOURNAL ARTICLE

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