Publications
277 results found
Collins JW, Meganck JA, Kuo C, et al., 2013, 4D Multimodality Imaging of <i>Citrobacter rodentium</i> Infections in Mice, JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, ISSN: 1940-087X
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- Citations: 11
Harding CR, Stoneham CA, Schuelein R, et al., 2013, The Dot/Icm Effector SdhA Is Necessary for Virulence of <i>Legionella pneumophila</i> in <i>Galleria mellonella</i> and A/J Mice, INFECTION AND IMMUNITY, Vol: 81, Pages: 2598-2605, ISSN: 0019-9567
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- Citations: 34
Aurass P, Schlegel M, Metwally O, et al., 2013, The <i>Legionella pneumophila</i> Dot/Icm-secreted Effector PlcC/CegC1 Together with PlcA and PlcB Promotes Virulence and Belongs to a Novel Zinc Metallophospholipase C Family Present in Bacteria and Fungi, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 288, Pages: 11080-11092
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- Citations: 35
Clare S, John V, Walker AW, et al., 2013, Enhanced Susceptibility to <i>Citrobacter rodentium</i> Infection in MicroRNA-155-Deficient Mice, INFECTION AND IMMUNITY, Vol: 81, Pages: 723-732, ISSN: 0019-9567
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- Citations: 29
Clements A, Berger CN, Lomma M, et al., 2013, Type 3 secretion effectors, Escherichia coliPathotypes and Principles of Pathogenesis, Editors: Donnenberg, ISBN: 978-0-12-397048-0
Vogrin AJ, Mousnier A, Frankel G, et al., 2013, Subcellular localization of legionella Dot/Icm effectors., Methods Mol Biol, Vol: 954, Pages: 333-344
The translocation of effector proteins by the Dot/Icm type IV secretion system is central to the ability of Legionella pneumophila to persist and replicate within eukaryotic cells. The subcellular localization of translocated Dot/Icm proteins in host cells provides insight into their function. Through co-staining with host cell markers, effector proteins may be localized to specific subcellular compartments and membranes, which frequently reflects their host cell target and mechanism of action. In this chapter, we describe protocols to (1) localize effector proteins within cells by ectopic expression using green fluorescent protein fusions and (2) localize effector proteins within infected cells using epitope-tagged effector proteins and immuno-fluorescence microscopy.
Dart AE, Tollis S, Bright MD, et al., 2012, The motor protein myosin 1G functions in FcγR-mediated phagocytosis, JOURNAL OF CELL SCIENCE, Vol: 125, Pages: 6020-6029, ISSN: 0021-9533
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- Citations: 34
Collins JW, Akin AR, Kosta A, et al., 2012, Pre-treatment with <i>Bifidobacterium breve</i> UCC2003 modulates <i>Citrobacter rodentium</i>-induced colonic inflammation and organ specificity, MICROBIOLOGY-SGM, Vol: 158, Pages: 2826-2834, ISSN: 1350-0872
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- Citations: 15
Berger CN, Crepin VF, Baruch K, et al., 2012, EspZ of Enteropathogenic and Enterohemorrhagic Escherichia coli Regulates Type III Secretion System Protein Translocation, mBio, Vol: 3, ISSN: 2161-2129
Translocation of effector proteins via a type III secretion system (T3SS) is a widespread infection strategy among Gram-negative bacterial pathogens. Each pathogen translocates a particular set of effectors that subvert cell signaling in a way that suits its particular infection cycle. However, as effector unbalance might lead to cytotoxicity, the pathogens must employ mechanisms that regulate the intracellular effector concentration. We present evidence that the effector EspZ controls T3SS effector translocation from enteropathogenic (EPEC) and enterohemorrhagic (EHEC) Escherichia coli. Consistently, an EPEC espZ mutant is highly cytotoxic. Following ectopic expression, we found that EspZ inhibited the formation of actin pedestals as it blocked the translocation of Tir, as well as other effectors, including Map and EspF. Moreover, during infection EspZ inhibited effector translocation following superinfection. Importantly, while EspZ of EHEC O157:H7 had a universal “translocation stop” activity, EspZ of EPEC inhibited effector translocation from typical EPEC strains but not from EHEC O157:H7 or its progenitor, atypical EPEC O55:H7. We found that the N and C termini of EspZ, which contains two transmembrane domains, face the cytosolic leaflet of the plasma membrane at the site of bacterial attachment, while the extracellular loop of EspZ is responsible for its strain-specific activity. These results show that EPEC and EHEC acquired a sophisticated mechanism to regulate the effector translocation.
Munera D, Martinez E, Varyukhina S, et al., 2012, Recruitment and membrane interactions of host cell proteins during attachment of enteropathogenic and enterohaemorrhagic <i>Escherichia coli</i>, BIOCHEMICAL JOURNAL, Vol: 445, Pages: 383-392, ISSN: 0264-6021
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- Citations: 13
Harding CR, Schroeder GN, Reynolds S, et al., 2012, <i>Legionella pneumophila</i> Pathogenesis in the <i>Galleria mellonella</i> Infection Model, INFECTION AND IMMUNITY, Vol: 80, Pages: 2780-2790, ISSN: 0019-9567
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- Citations: 82
Wong ARC, Raymond B, Collins JW, et al., 2012, The enteropathogenic E. coli effector EspH promotes actin pedestal formation and elongation via WASP-interacting protein (WIP), CELLULAR MICROBIOLOGY, Vol: 14, Pages: 1051-1070, ISSN: 1462-5814
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- Citations: 24
Nesta B, Spraggon G, Alteri C, et al., 2012, FdeC, a Novel Broadly Conserved <i>Escherichia coli</i> Adhesin Eliciting Protection against Urinary Tract Infections, MBIO, Vol: 3, ISSN: 2150-7511
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- Citations: 85
Wong ARC, Clements A, Raymond B, et al., 2012, The Interplay between the Escherichia coli Rho Guanine Nucleotide Exchange Factor Effectors and the Mammalian RhoGEF Inhibitor EspH, mBio, Vol: 3, ISSN: 2161-2129
Rho GTPases are important regulators of many cellular processes. Subversion of Rho GTPases is a common infection strategy employed by many important human pathogens. Enteropathogenic Escherichia coli and enterohemorrhagic Escherichia coli (EPEC and EHEC) translocate the effector EspH, which inactivates mammalian Rho guanine exchange factors (GEFs), as well as Map, EspT, and EspM2, which, by mimicking mammalian RhoGEFs, activate Rho GTPases. In this study we found that EspH induces focal adhesion disassembly, triggers cell detachment, activates caspase-3, and induces cytotoxicity. EspH-induced cell detachment and caspase-3 activation can be offset by EspT, EspM2, and the Salmonella Cdc42/Rac1 GEF effector SopE, which remain active in the presence of EspH. EPEC and EHEC therefore use a novel strategy of controlling Rho GTPase activity by translocating one effector to inactivate mammalian RhoGEFs, replacing them with bacterial RhoGEFs. This study also expands the functional range of bacterial RhoGEFs to include cell adhesion and survival.IMPORTANCE Many human pathogens use a type III secretion system to translocate effectors that can functionally be divided into signaling, disabling, and countervirulence effectors. Among the signaling effectors are those that activate Rho GTPases, which play a central role in coordinating actin dynamics. However, many pathogens also translocate effectors with antagonistic or counteractive functions. For example, Salmonella translocates SopE and SptP, which sequentially turn Rac1 and Cdc42 on and off. In this paper, we show that enteropathogenic E. coli translocates EspH, which inactivates mammalian RhoGEFs and triggers cytotoxicity and at the same time translocates the bacterial RhoGEFs EspM2 and EspT, which are insensitive to EspH, and so neutralizes EspH-induced focal adhesion disassembly, cell detachment, and caspase-3 activation. Our data point to an intriguing infection strategy in which EPEC and EHEC override cellular Rho GTPase
Islam MS, Shaw RK, Frankel G, et al., 2012, Translation of a minigene in the 5′ leader sequence of the enterohaemorrhagic <i>Escherichia coli</i> <i>LEE1</i> transcription unit affects expression of the neighbouring downstream gene, BIOCHEMICAL JOURNAL, Vol: 441, Pages: 247-253, ISSN: 0264-6021
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- Citations: 11
Dolezal P, Aili M, Tong J, et al., 2012, <i>Legionella pneumophila</i> Secretes a Mitochondrial Carrier Protein during Infection, PLOS PATHOGENS, Vol: 8, ISSN: 1553-7366
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- Citations: 54
Clements A, Young JC, Constantinou N, et al., 2012, Infection strategies of enteric pathogenic <i>Escherichia coli</i>, GUT MICROBES, Vol: 3, Pages: 71-87, ISSN: 1949-0976
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- Citations: 190
Bright MD, Frankel G, 2011, PAK4 phosphorylates myosin regulatory light chain and contributes to Fcγ receptor-mediated phagocytosis, INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, Vol: 43, Pages: 1776-1781, ISSN: 1357-2725
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- Citations: 11
Raymond B, Crepin VF, Collins JW, et al., 2011, The WxxxE effector EspT triggers expression of immune mediators in an Erk/JNK and NF-κB-dependent manner, CELLULAR MICROBIOLOGY, Vol: 13, Pages: 1881-1893, ISSN: 1462-5814
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- Citations: 35
Muangman S, Korbsrisate S, Muangsombut V, et al., 2011, BopC is a type III secreted effector protein of <i>Burkholderia pseudomallei</i>, FEMS MICROBIOLOGY LETTERS, Vol: 323, Pages: 75-82, ISSN: 0378-1097
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- Citations: 12
Shaw RK, Lasa I, Garcia BM, et al., 2011, Cellulose mediates attachment of <i>Salmonella enterica</i> Serovar Typhimurium to tomatoes, ENVIRONMENTAL MICROBIOLOGY REPORTS, Vol: 3, Pages: 569-573, ISSN: 1758-2229
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- Citations: 19
Clements A, Smollett K, Lee SF, et al., 2011, EspG of enteropathogenic and enterohemorrhagic <i>E</i>. <i>coli</i> binds the Golgi matrix protein GM130 and disrupts the Golgi structure and function, CELLULAR MICROBIOLOGY, Vol: 13, Pages: 1429-1439, ISSN: 1462-5814
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- Citations: 30
Fookes M, Schroeder GN, Langridge GC, et al., 2011, Salmonella bongori Provides Insights into the Evolution of the Salmonellae, PLOS Pathogens, Vol: 7, ISSN: 1553-7366
The genus Salmonella contains two species, S. bongori and S. enterica. Compared to the well-studied S. enterica there is a marked lack of information regarding the genetic makeup and diversity of S. bongori. S. bongori has been found predominantly associated with cold-blooded animals, but it can infect humans. To define the phylogeny of this species, and compare it to S. enterica, we have sequenced 28 isolates representing most of the known diversity of S. bongori. This cross-species analysis allowed us to confidently differentiate ancestral functions from those acquired following speciation, which include both metabolic and virulence-associated capacities. We show that, although S. bongori inherited a basic set of Salmonella common virulence functions, it has subsequently elaborated on this in a different direction to S. enterica. It is an established feature of S. enterica evolution that the acquisition of the type III secretion systems (T3SS-1 and T3SS-2) has been followed by the sequential acquisition of genes encoding secreted targets, termed effectors proteins. We show that this is also true of S. bongori, which has acquired an array of novel effector proteins (sboA-L). All but two of these effectors have no significant S. enterica homologues and instead are highly similar to those found in enteropathogenic Escherichia coli (EPEC). Remarkably, SboH is found to be a chimeric effector protein, encoded by a fusion of the T3SS-1 effector gene sopA and a gene highly similar to the EPEC effector nleH from enteropathogenic E. coli. We demonstrate that representatives of these new effectors are translocated and that SboH, similarly to NleH, blocks intrinsic apoptotic pathways while being targeted to the mitochondria by the SopA part of the fusion. This work suggests that S. bongori has inherited the ancestral Salmonella virulence gene set, but has adapted by incorporating virulence determinants that resemble those employed by EPEC.
Wong ARC, Pearson JS, Bright MD, et al., 2011, Enteropathogenic and enterohaemorrhagic <i>Escherichia coli</i>: even more subversive elements, MOLECULAR MICROBIOLOGY, Vol: 80, Pages: 1420-1438, ISSN: 0950-382X
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- Citations: 254
Berger CN, Brown DJ, Shaw RK, et al., 2011, <i>Salmonella enterica</i> strains belonging to O serogroup 1,3,19 induce chlorosis and wilting of <i>Arabidopsis thaliana</i> leaves, ENVIRONMENTAL MICROBIOLOGY, Vol: 13, Pages: 1299-1308, ISSN: 1462-2912
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- Citations: 25
Robinson KS, Clements A, Williams AC, et al., 2011, Bax Inhibitor 1 in apoptosis and disease, ONCOGENE, Vol: 30, Pages: 2391-2400, ISSN: 0950-9232
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- Citations: 61
Pearson JS, Riedmaier P, Marches O, et al., 2011, A type III effector protease NleC from enteropathogenic <i>Escherichia coli</i> targets NF-κB for degradation, MOLECULAR MICROBIOLOGY, Vol: 80, Pages: 219-230, ISSN: 0950-382X
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- Citations: 102
Petty NK, Feltwell T, Pickard D, et al., 2011, <i>Citrobacter rodentium</i> is an Unstable Pathogen Showing Evidence of Significant Genomic Flux, PLOS PATHOGENS, Vol: 7, ISSN: 1553-7374
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- Citations: 27
Arbeloa A, Oates CV, Marches O, et al., 2011, Enteropathogenic and Enterohemorrhagic <i>Escherichia coli</i> Type III Secretion Effector EspV Induces Radical Morphological Changes in Eukaryotic Cells, INFECTION AND IMMUNITY, Vol: 79, Pages: 1067-1076, ISSN: 0019-9567
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- Citations: 19
Shaw RK, Berger CN, Pallen MJ, et al., 2011, Flagella mediate attachment of enterotoxigenic <i>Escherichia coli</i> to fresh salad leaves, ENVIRONMENTAL MICROBIOLOGY REPORTS, Vol: 3, Pages: 112-117, ISSN: 1758-2229
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- Citations: 21
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