Imperial College London
Alternate

ProfessorGeorgeHanna

Faculty of Medicine, Department of Surgery & Cancer

Head of Department of Surgery and Cancer
 
 
 
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Contact

 

+44 (0)20 7594 3396g.hanna

 
 
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Assistant

 

Ms Aoibheann Byrne +44 (0)20 7594 3396

 
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Location

 

Block B Hammersmith HospitalHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Doran:2017:1752-7163/aa8a46,
author = {Doran, SLF and Romano, A and hanna, GB},
doi = {1752-7163/aa8a46},
journal = {Journal of Breath Research},
title = {Optimisation of sampling parameters for standardised exhaled breath sampling},
url = {http://dx.doi.org/10.1088/1752-7163/aa8a46},
volume = {12},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The lack of standardization of breath sampling is a major contributing factor to the poor repeatability of results and hence represents a barrier to the adoption of breath tests in clinical practice. On-line and bag breath sampling have advantages but do not suit multicentre clinical studies whereas storage and robust transport are essential for the conduct of wide-scale studies. Several devices have been developed to control sampling parameters and to concentrate volatile organic compounds (VOCs) onto thermal desorption (TD) tubes and subsequently transport those tubes for laboratory analysis. We conducted three experiments to investigate (i) the fraction of breath sampled (whole vs. lower expiratory exhaled breath); (ii) breath sample volume (125, 250, 500 and 1000ml) and (iii) breath sample flow rate (400, 200, 100 and 50 ml/min). The target VOCs were acetone and potential volatile biomarkers for oesophago-gastric cancer belonging to the aldehyde, fatty acids and phenol chemical classes. We also examined the collection execution time and the impact of environmental contamination. The experiments showed that the use of exhaled breath-sampling devices requires the selection of optimum sampling parameters. The increase in sample volume has improved the levels of VOCs detected. However, the influence of the fraction of exhaled breath and the flow rate depends on the target VOCs measured. The concentration of potential volatile biomarkers for oesophago-gastric cancer was not significantly different between the whole and lower airway exhaled breath. While the recovery of phenols and acetone from TD tubes was lower when breath sampling was performed at a higher flow rate, other VOCs were not affected. A dedicated ‘clean air supply’ reduces the contamination from ambient air, but the breath collection device itself can be a source of contaminants. In clinical studies using VOCs to elicit potential biomarkers of gastro-oesophageal cancer, the optimum parameter
AU  - Doran,SLF
AU  - Romano,A
AU  - hanna,GB
DO  - 1752-7163/aa8a46
PY  - 2017///
SN  - 1752-7155
TI  - Optimisation of sampling parameters for standardised exhaled breath sampling
T2  - Journal of Breath Research
UR  - http://dx.doi.org/10.1088/1752-7163/aa8a46
UR  - http://hdl.handle.net/10044/1/51217
VL  - 12
ER  -
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