Imperial College London

DrGeraldLarrouy-Maumus

Faculty of Natural SciencesDepartment of Life Sciences

Lecturer in Molecular Microbiology
 
 
 
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Contact

 

+44 (0)20 7594 7463g.larrouy-maumus

 
 
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Location

 

3.42Flowers buildingSouth Kensington Campus

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Summary

 

Publications

Publication Type
Year
to

32 results found

Berger CN, Crepin VF, Roumeliotis TI, Wright JC, Carson D, Pevsner-Fischer M, Furniss RCD, Dougan G, Bachash M, Yu L, Clements A, Collins JW, Elinav E, Larrouy-Maumus GJ, Choudhary JS, Frankel Get al., 2017, Citrobacter rodentium Subverts ATP Flux and Cholesterol Homeostasis in Intestinal Epithelial Cells In Vivo., Cell Metab

The intestinal epithelial cells (IECs) that line the gut form a robust line of defense against ingested pathogens. We investigated the impact of infection with the enteric pathogen Citrobacter rodentium on mouse IEC metabolism using global proteomic and targeted metabolomics and lipidomics. The major signatures of the infection were upregulation of the sugar transporter Sglt4, aerobic glycolysis, and production of phosphocreatine, which mobilizes cytosolic energy. In contrast, biogenesis of mitochondrial cardiolipins, essential for ATP production, was inhibited, which coincided with increased levels of mucosal O2 and a reduction in colon-associated anaerobic commensals. In addition, IECs responded to infection by activating Srebp2 and the cholesterol biosynthetic pathway. Unexpectedly, infected IECs also upregulated the cholesterol efflux proteins AbcA1, AbcG8, and ApoA1, resulting in higher levels of fecal cholesterol and a bloom of Proteobacteria. These results suggest that C. rodentium manipulates host metabolism to evade innate immune responses and establish a favorable gut ecosystem.

JOURNAL ARTICLE

Buchieri MV, Cimino M, Rebollo-Ramirez S, Beauvineau C, Cascioferro A, Favre-Rochex S, Helynck O, Naud-Martin D, Larrouy-Maumus G, Munier-Lehmann H, Gicquel Bet al., 2017, Nitazoxanide Analogs Require Nitroreduction for Antimicrobial Activity in Mycobacterium smegmatis, JOURNAL OF MEDICINAL CHEMISTRY, Vol: 60, Pages: 7425-7433, ISSN: 0022-2623

JOURNAL ARTICLE

Larrouy-Maumus G, Layre E, Clark S, Prandi J, Rayner E, Lepore M, de Libero G, Williams A, Puzo G, Gilleron Met al., 2017, Protective efficacy of a lipid antigen vaccine in a guinea pig model of tuberculosis, VACCINE, Vol: 35, Pages: 1395-1402, ISSN: 0264-410X

JOURNAL ARTICLE

Pacholarz KJ, Burnley RJ, Jowitt TA, Ordsmith V, Pisco JP, Porrini M, Larrouy-Maumus G, Garlish RA, Taylor RJ, de Carvalho LPS, Barran PEet al., 2017, Hybrid Mass Spectrometry Approaches to Determine How L-Histidine Feedback Regulates the Enzyzme MtATP-Phosphoribosyltransferase, STRUCTURE, Vol: 25, Pages: 730-+, ISSN: 0969-2126

JOURNAL ARTICLE

Robert CB, Thomson M, Vercellone A, Gardner F, Ernst RK, Larrouy-Maumus G, Nigou Jet al., 2017, Mass spectrometry analysis of intact Francisella bacteria identifies lipid A structure remodeling in response to acidic pH stress., Biochimie, Vol: 141, Pages: 16-20

Structural modification of lipid A, the lipid anchor of LPS, is one of the strategies used by Gram-negative bacteria to evade host innate immunity. Francisella tularensis is a human pathogen that infects and replicates within phagocytic cells. It produces an atypical lipid A, whose structure precludes an efficient recognition by both innate immune players, TLR4 and cationic antimicrobial peptides. Interestingly, a recent report indicates that the lipid A of Francisella (LVS vaccinal strain) undergoes polar modifications when bacteria are grown in human macrophages as compared to in broth. To characterize the structural modifications of lipid A that may be induced intracellularly, Francisella novicida, a surrogate strain for the highly virulent F. tularensis, was submitted to different stress conditions mimicking the harsh environment encountered in the macrophages. To analyze lipid A directly from intact bacteria without any chemical treatment or purification steps, we used a rapid and sensitive MALDI-TOF mass spectrometry approach. Among the many conditions tested, only bacteria exposure to acidic pHs (from 6 to 5) induced a change in lipid A structure. These changes were characterized by an increase in the relative abundance of molecular species bearing an additional hexose unit on the diglucosamine backbone, similar to species present when bacteria are grown under reduced environmental temperature. This lipid A glyco-form, which is observed in trace amounts in normal in vitro growth conditions at 37 °C, may contribute to the intracellular parasitism of macrophages by Francisella.

JOURNAL ARTICLE

Larrouy-Maumus G, Clements A, Filloux A, McCarthy RR, Mostowy Set al., 2016, Direct detection of lipid A on intact Gram-negative bacteria by MALDI-TOF mass spectrometry, JOURNAL OF MICROBIOLOGICAL METHODS, Vol: 120, Pages: 68-71, ISSN: 0167-7012

JOURNAL ARTICLE

Larrouy-Maumus G, Marino LB, Madduri AVR, Ragan TJ, Hunt DM, Bassano L, Gutierrez MG, Moody DB, Pavan FR, de Carvalho LPSet al., 2016, Cell-Envelope Remodeling as a Determinant of Phenotypic Antibacterial Tolerance in Mycobacterium tuberculosis, ACS INFECTIOUS DISEASES, Vol: 2, Pages: 352-360, ISSN: 2373-8227

JOURNAL ARTICLE

Sirianni A, Krokowski S, Lobato-Marquez D, Buranyi S, Pfanzelter J, Galea D, Willis A, Culley S, Henriques R, Larrouy-Maumus G, Hollinshead M, Sancho-Shimizu V, Way M, Mostowy Set al., 2016, Mitochondria mediate septin cage assembly to promote autophagy of Shigella, EMBO REPORTS, Vol: 17, Pages: 1029-1043, ISSN: 1469-221X

JOURNAL ARTICLE

Larrouy-Maumus G, 2015, Cholesterol acquisition by Mycobacterium tuberculosis, VIRULENCE, Vol: 6, Pages: 412-413, ISSN: 2150-5594

JOURNAL ARTICLE

Larrouy-Maumus G, Gilleron M, Skovierova H, Zuberogoitia S, Brennan PJ, Puzo G, Jackson M, Nigou Jet al., 2015, A glycomic approach reveals a new mycobacterial polysaccharide, GLYCOBIOLOGY, Vol: 25, Pages: 1163-1171, ISSN: 0959-6658

JOURNAL ARTICLE

Larrouy-Maumus G, Puzo G, 2015, Mycobacterial envelope lipids fingerprint from direct MALDI-TOF MS analysis of intact bacilli, TUBERCULOSIS, Vol: 95, Pages: 75-85, ISSN: 1472-9792

JOURNAL ARTICLE

Wheat WH, Dhouib R, Angala SK, Larrouy-Maumus G, Dobos K, Nigou J, Spencer JS, Jackson Met al., 2015, The presence of a galactosamine substituent on the arabinogalactan of Mycobacterium tuberculosis abrogates full maturation of human peripheral blood monocyte-derived dendritic cells and increases secretion of IL-10, TUBERCULOSIS, Vol: 95, Pages: 476-489, ISSN: 1472-9792

JOURNAL ARTICLE

Belardinelli JM, Larrouy-Maumus G, Jones V, de Carvalho LPS, McNeil MR, Jackson Met al., 2014, Biosynthesis and Translocation of Unsulfated Acyltrehaloses in Mycobacterium tuberculosis, Journal of Biological Chemistry, Vol: 289, Pages: 27952-27965, ISSN: 1083-351X

JOURNAL ARTICLE

Gouzy A, Larrouy-Maumus G, Bottai D, Levillain F, Dumas A, Wallach JB, Caire-Brandli I, de Chastellier C, Wu T-D, Poincloux R, Brosch R, Guerquin-Kern J-L, Schnappinger D, de Carvalho LPS, Poquet Y, Neyrolles Oet al., 2014, Mycobacterium tuberculosis Exploits Asparagine to Assimilate Nitrogen and Resist Acid Stress during Infection, PLOS PATHOGENS, Vol: 10, ISSN: 1553-7374

JOURNAL ARTICLE

Larrouy-Maumus G, Kelly G, de Carvalho LPS, 2014, Chemical Mechanism of Glycerol 3-Phosphate Phosphatase: pH-Dependent Changes in the Rate-Limiting Step, BIOCHEMISTRY, Vol: 53, Pages: 143-151, ISSN: 0006-2960

JOURNAL ARTICLE

Prosser GA, Larrouy-Maumus G, de Carvalho LPS, 2014, Metabolomic strategies for the identification of new enzyme functions and metabolic pathways, EMBO REPORTS, Vol: 15, Pages: 657-669, ISSN: 1469-221X

JOURNAL ARTICLE

Gouzy A, Larrouy-Maumus G, Wu T-D, Peixoto A, Levillain F, Lugo-Villarino G, Guerquin-Kern J-L, de Carvalho LPS, Poquet Y, Neyrolles Oet al., 2013, Mycobacterium tuberculosis nitrogen assimilation and host colonization require aspartate, NATURE CHEMICAL BIOLOGY, Vol: 9, Pages: 674-+, ISSN: 1552-4450

JOURNAL ARTICLE

Larrouy-Maumus G, Biswas T, Hunt DM, Kelly G, Tsodikov OV, de Carvalho LPSet al., 2013, Discovery of a glycerol 3-phosphate phosphatase reveals glycerophospholipid polar head recycling in Mycobacterium tuberculosis, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 110, Pages: 11320-11325, ISSN: 0027-8424

JOURNAL ARTICLE

Mao C, Shukla M, Larrouy-Maumus G, Dix FL, Kelley LA, Sternberg MJ, Sobral BW, de Carvalho LPSet al., 2013, Functional assignment of Mycobacterium tuberculosis proteome revealed by genome-scale fold-recognition, TUBERCULOSIS, Vol: 93, Pages: 40-46, ISSN: 1472-9792

JOURNAL ARTICLE

Jackson M, Dhouib R, Skovierova H, Larrouy-Maumus G, Angala SK, Wheat WH, Ha P, Gilleron M, Brennan PJ, Puzo G, Nigou Jet al., 2012, Biogenesis of mycobacterial cell envelope glycoconjugates, Experimental Biology Meeting, Publisher: FEDERATION AMER SOC EXP BIOL, ISSN: 0892-6638

CONFERENCE PAPER

Larrouy-Maumus G, Skovierova H, Dhouib R, Angala SK, Zuberogoitia S, Ha P, Villela AD, Mikusova K, Noguera A, Gilleron M, Valentinova L, Kordulakova J, Brennan PJ, Puzo G, Nigou J, Jackson Met al., 2012, A Small Multidrug Resistance-like Transporter Involved in the Arabinosylation of Arabinogalactan and Lipoarabinomannan in Mycobacteria, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 287, ISSN: 0021-9258

JOURNAL ARTICLE

Pedreno S, Pisco JP, Larrouy-Maumus G, Kelly G, de Carvalho LPSet al., 2012, Mechanism of Feedback Allosteric Inhibition of ATP Phosphoribosyltransferase, BIOCHEMISTRY, Vol: 51, Pages: 8027-8038, ISSN: 0006-2960

JOURNAL ARTICLE

Cot M, Ray A, Gilleron M, Vercellone A, Larrouy-Maumus G, Armau E, Gauthier S, Tiraby G, Puzo G, Nigou Jet al., 2011, Lipoteichoic Acid in Streptomyces hygroscopicus: Structural Model and Immunomodulatory Activities, PLOS ONE, Vol: 6, ISSN: 1932-6203

JOURNAL ARTICLE

Krishna S, Ray A, Dubey SK, Larrouy-Maumus G, Chalut C, Castanier R, Noguera A, Gilleron M, Puzo G, Vercellone A, Nampoothiri KM, Nigou Jet al., 2011, Lipoglycans Contribute to Innate Immune Detection of Mycobacteria, PLOS ONE, Vol: 6, ISSN: 1932-6203

JOURNAL ARTICLE

Layre E, Cala-De Paepe D, Larrouy-Maumus G, Vaubourgeix J, Mundayoor S, Lindner B, Puzo G, Gilleron Met al., 2011, Deciphering sulfoglycolipids of Mycobacterium tuberculosis, JOURNAL OF LIPID RESEARCH, Vol: 52, Pages: 1098-1110, ISSN: 0022-2275

JOURNAL ARTICLE

Brodin P, Poquet Y, Levillain F, Peguillet I, Larrouy-Maumus G, Gilleron M, Ewann F, Christophe T, Fenistein D, Jang J, Jang M-S, Park S-J, Rauzier J, Carralot J-P, Shrimpton R, Genovesio A, Gonzalo-Asensio JA, Puzo G, Martin C, Brosch R, Stewart GR, Gicquel B, Neyrolles Oet al., 2010, High Content Phenotypic Cell-Based Visual Screen Identifies Mycobacterium tuberculosis Acyltrehalose-Containing Glycolipids Involved in Phagosome Remodeling, PLOS PATHOGENS, Vol: 6, ISSN: 1553-7366

JOURNAL ARTICLE

Driessen NN, Stoop EJM, Ummels R, Gurcha SS, Mishra AK, Larrouy-Maumus G, Nigou J, Gilleron M, Puzo G, Maaskant JJ, Sparrius M, Besra GS, Bitter W, Vandenbroucke-Grauls CMJE, Appelmelk BJet al., 2010, Mycobacterium marinum MMAR_2380, a predicted transmembrane acyltransferase, is essential for the presence of the mannose cap on lipoarabinomannan, MICROBIOLOGY-SGM, Vol: 156, Pages: 3492-3502, ISSN: 1350-0872

JOURNAL ARTICLE

Skovierova H, Larrouy-Maumus G, Pham H, Belanova M, Barilone N, DasGupta A, Mikusova K, Gicquel B, Gilleron M, Brennan PJ, Puzo G, Nigou J, Jackson Met al., 2010, Biosynthetic Origin of the Galactosamine Substituent of Arabinogalactan in Mycobacterium tuberculosis, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 285, Pages: 41348-41355, ISSN: 0021-9258

JOURNAL ARTICLE

Kaur D, Pham H, Larrouy-Maumus G, Rivière M, Vissa V, Guerin ME, Puzo G, Brennan PJ, Jackson Met al., 2009, Initiation of methylglucose lipopolysaccharide biosynthesis in mycobacteria., PLoS One, Vol: 4

BACKGROUND: Mycobacteria produce two unique families of cytoplasmic polymethylated polysaccharides -- the methylglucose lipopolysaccharides (MGLPs) and the methylmannose polysaccharides (MMPs) -- the physiological functions of which are still poorly defined. Towards defining the roles of these polysaccharides in mycobacterial physiology, we generated knock-out mutations of genes in their putative biosynthetic pathways. METHODOLOGY/PRINCIPAL FINDINGS: We report here on the characterization of the Rv1208 protein of Mycobacterium tuberculosis and its ortholog in Mycobacterium smegmatis (MSMEG_5084) as the enzymes responsible for the transfer of the first glucose residue of MGLPs. Disruption of MSMEG_5084 in M. smegmatis resulted in a dramatic decrease in MGLP synthesis directly attributable to the almost complete abolition of glucosyl-3-phosphoglycerate synthase activity in this strain. Synthesis of MGLPs in the mutant was restored upon complementation with wild-type copies of the Rv1208 gene from M. tuberculosis or MSMEG_5084 from M. smegmatis. CONCLUSIONS/SIGNIFICANCE: This is the first evidence linking Rv1208 to MGLP biosynthesis. Thus, the first step in the initiation of MGLP biosynthesis in mycobacteria has been defined, and subsequent steps can be inferred.

JOURNAL ARTICLE

Skovierova H, Larrouy-Maumus G, Zhang J, Kaur D, Barilone N, Kordulakova J, Gilleron M, Guadagnini S, Belanova M, Prevost M-C, Gicquel B, Puzo G, Chatterjee D, Brennan PJ, Nigou J, Jackson Met al., 2009, AftD, a novel essential arabinofuranosyltransferase from mycobacteria, GLYCOBIOLOGY, Vol: 19, Pages: 1235-1247, ISSN: 0959-6658

JOURNAL ARTICLE

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