35 results found
Skovierova H, Larrouy-Maumus G, Pham H, et al., 2010, Biosynthetic Origin of the Galactosamine Substituent of Arabinogalactan in Mycobacterium tuberculosis, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 285, Pages: 41348-41355, ISSN: 0021-9258
Kaur D, Pham H, Larrouy-Maumus G, et al., 2009, Initiation of methylglucose lipopolysaccharide biosynthesis in mycobacteria., PLoS One, Vol: 4
BACKGROUND: Mycobacteria produce two unique families of cytoplasmic polymethylated polysaccharides -- the methylglucose lipopolysaccharides (MGLPs) and the methylmannose polysaccharides (MMPs) -- the physiological functions of which are still poorly defined. Towards defining the roles of these polysaccharides in mycobacterial physiology, we generated knock-out mutations of genes in their putative biosynthetic pathways. METHODOLOGY/PRINCIPAL FINDINGS: We report here on the characterization of the Rv1208 protein of Mycobacterium tuberculosis and its ortholog in Mycobacterium smegmatis (MSMEG_5084) as the enzymes responsible for the transfer of the first glucose residue of MGLPs. Disruption of MSMEG_5084 in M. smegmatis resulted in a dramatic decrease in MGLP synthesis directly attributable to the almost complete abolition of glucosyl-3-phosphoglycerate synthase activity in this strain. Synthesis of MGLPs in the mutant was restored upon complementation with wild-type copies of the Rv1208 gene from M. tuberculosis or MSMEG_5084 from M. smegmatis. CONCLUSIONS/SIGNIFICANCE: This is the first evidence linking Rv1208 to MGLP biosynthesis. Thus, the first step in the initiation of MGLP biosynthesis in mycobacteria has been defined, and subsequent steps can be inferred.
Skovierova H, Larrouy-Maumus G, Zhang J, et al., 2009, AftD, a novel essential arabinofuranosyltransferase from mycobacteria, GLYCOBIOLOGY, Vol: 19, Pages: 1235-1247, ISSN: 0959-6658
Appelmelk BJ, den Dunnen J, Driessen NN, et al., 2008, The mannose cap of mycobacterial lipoarabinomannan does not dominate the Mycobacterium-host interaction, CELLULAR MICROBIOLOGY, Vol: 10, Pages: 930-944, ISSN: 1462-5814
Pitarque S, Larrouy-Maumus G, Payre B, et al., 2008, The immunomodulatory lipoglycans, lipoarabinomannan and lipomannan, are exposed at the mycobacterial cell surface, TUBERCULOSIS, Vol: 88, Pages: 560-565, ISSN: 1472-9792
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