Imperial College London

DrGeraldLarrouy-Maumus

Faculty of Natural SciencesDepartment of Life Sciences

Lecturer in Molecular Microbiology
 
 
 
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Contact

 

+44 (0)20 7594 7463g.larrouy-maumus

 
 
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Location

 

3.42Flowers buildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Kaur:2009:10.1371/journal.pone.0005447,
author = {Kaur, D and Pham, H and Larrouy-Maumus, G and Rivière, M and Vissa, V and Guerin, ME and Puzo, G and Brennan, PJ and Jackson, M},
doi = {10.1371/journal.pone.0005447},
journal = {PLoS One},
title = {Initiation of methylglucose lipopolysaccharide biosynthesis in mycobacteria.},
url = {http://dx.doi.org/10.1371/journal.pone.0005447},
volume = {4},
year = {2009}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - BACKGROUND: Mycobacteria produce two unique families of cytoplasmic polymethylated polysaccharides -- the methylglucose lipopolysaccharides (MGLPs) and the methylmannose polysaccharides (MMPs) -- the physiological functions of which are still poorly defined. Towards defining the roles of these polysaccharides in mycobacterial physiology, we generated knock-out mutations of genes in their putative biosynthetic pathways. METHODOLOGY/PRINCIPAL FINDINGS: We report here on the characterization of the Rv1208 protein of Mycobacterium tuberculosis and its ortholog in Mycobacterium smegmatis (MSMEG_5084) as the enzymes responsible for the transfer of the first glucose residue of MGLPs. Disruption of MSMEG_5084 in M. smegmatis resulted in a dramatic decrease in MGLP synthesis directly attributable to the almost complete abolition of glucosyl-3-phosphoglycerate synthase activity in this strain. Synthesis of MGLPs in the mutant was restored upon complementation with wild-type copies of the Rv1208 gene from M. tuberculosis or MSMEG_5084 from M. smegmatis. CONCLUSIONS/SIGNIFICANCE: This is the first evidence linking Rv1208 to MGLP biosynthesis. Thus, the first step in the initiation of MGLP biosynthesis in mycobacteria has been defined, and subsequent steps can be inferred.
AU - Kaur,D
AU - Pham,H
AU - Larrouy-Maumus,G
AU - Rivière,M
AU - Vissa,V
AU - Guerin,ME
AU - Puzo,G
AU - Brennan,PJ
AU - Jackson,M
DO - 10.1371/journal.pone.0005447
PY - 2009///
TI - Initiation of methylglucose lipopolysaccharide biosynthesis in mycobacteria.
T2 - PLoS One
UR - http://dx.doi.org/10.1371/journal.pone.0005447
UR - https://www.ncbi.nlm.nih.gov/pubmed/19421329
VL - 4
ER -