Imperial College London

DrGeraldLarrouy-Maumus

Faculty of Natural SciencesDepartment of Life Sciences

Lecturer in Molecular Microbiology
 
 
 
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Contact

 

+44 (0)20 7594 7463g.larrouy-maumus

 
 
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Location

 

3.42Flowers buildingSouth Kensington Campus

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Summary

 

Publications

Publication Type
Year
to

44 results found

Rebollo-Ramirez S, Larrouy-Maumus G, 2019, NaCl triggers the CRP-dependent increase of cAMP in Mycobacterium tuberculosis, Tuberculosis, Vol: 116, Pages: 8-16, ISSN: 1472-9792

JOURNAL ARTICLE

Lobato-Márquez D, Krokowski S, Sirianni A, Larrouy-Maumus G, Mostowy Set al., 2019, A requirement for septins and the autophagy receptor p62 in the proliferation of intracellular Shigella., Cytoskeleton (Hoboken), Vol: 76, Pages: 163-172

Shigella flexneri, a Gram-negative enteroinvasive pathogen, causes inflammatory destruction of the human intestinal epithelium. During infection of epithelial cells, Shigella escape from the phagosome to the cytosol, where they reroute host cell glycolysis to obtain nutrients for proliferation. Septins, a poorly understood component of the cytoskeleton, can entrap cytosolic Shigella targeted to autophagy in cage-like structures to restrict bacterial proliferation. Although bacterial entrapment by septin caging has been the subject of intense investigation, the role of septins and the autophagy machinery in the proliferation of noncaged Shigella is mostly unknown. Here, we found that intracellular Shigella fail to efficiently proliferate in SEPT2-, SEPT7-, or p62/SQSTM1-depleted cells. Consistent with a failure to proliferate, single cell analysis of bacteria not entrapped in septin cages showed that the number of metabolically active Shigella in septin- or p62-depleted cells is reduced. Targeted metabolomic analysis revealed that host cell glycolysis is dysregulated in septin-depleted cells, suggesting a key role for septins in modulation of glycolysis. Together, these results suggest that septins and the autophagy machinery may regulate metabolic pathways that promote the proliferation of intracellular Shigella not entrapped in septin cages.

JOURNAL ARTICLE

Krokowski S, Lobato-Marquez D, Chastanet A, Pereira PM, Angelis D, Galea D, Larrouy-Maumus G, Henriques R, Spiliotis ET, Carballido-Lopez R, Mostowy Set al., 2018, Septins Recognize and Entrap Dividing Bacterial Cells for Delivery to Lysosomes, CELL HOST & MICROBE, Vol: 24, Pages: 866-+, ISSN: 1931-3128

JOURNAL ARTICLE

Dortet L, Bonnin RA, Pennisi I, Gauthier L, Jousset AB, Dabos L, Furniss RCD, Mavridou DAI, Bogaerts P, Glupczynski Y, Potron A, Plesiat P, Beyrouthy R, Robin F, Bonnet R, Naas T, Filloux A, Larrouy-Maumus Get al., 2018, Rapid detection and discrimination of chromosome-and MCR-plasmid-mediated resistance to polymyxins by MALDI-TOF MS in Escherichia coli: the MALDIxin test, JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, Vol: 73, Pages: 3359-3367, ISSN: 0305-7453

JOURNAL ARTICLE

Decout A, Silva-Gomes S, Drocourt D, Blattes E, Riviere M, Prandi J, Larrouy-Maumus G, Caminade A-M, Hamasur B, Kallenius G, Kaur D, Dobos KM, Lucas M, Sutcliffe IC, Besra GS, Appelmelk BJ, Gilleron M, Jackson M, Vercellone A, Tiraby G, Nigou Jet al., 2018, Deciphering the molecular basis of mycobacteria and lipoglycan recognition by the C-type lectin Dectin-2, SCIENTIFIC REPORTS, Vol: 8, ISSN: 2045-2322

JOURNAL ARTICLE

Dortet L, Potron A, Bonnin RA, Plesiat P, Naas T, Filloux A, Larrouy-Maumus Get al., 2018, Rapid detection of colistin resistance in Acinetobacter baumannii using MALDI-TOF-based lipidomics on intact bacteria, SCIENTIFIC REPORTS, Vol: 8, ISSN: 2045-2322

JOURNAL ARTICLE

Filloux A, Larrouy-Maumus G, Dortet L, 2018, Method of detection: Use of Lipid A and its modifications as a direct detection of antimicrobials resistance, WO2018158573

The present invention is directed to a method for detecting the presence or absence of a bacterium resistant to a cyclic cationic polypeptide antibiotic, comprising: (a) subjecting a test sample to mass spectrometry analysis and generating a mass spectrum output; wherein said test sample comprises a bacterial membrane or a fragment thereof, wherein the fragment comprises a non- Lipid A component; (b) identifying in said mass spectrum output a first defined peak indicative of the presence of Lipid A modified by phosphoethanolamine, wherein said first defined peak is a peak present in a mass spectrum output for Lipid A modified by phosphoethanolamine and wherein said first defined peak is absent from a corresponding mass spectrum output for native Lipid A; and (c) wherein the presence of said first defined peak indicates the presence of a bacterium resistant to a cyclic cationic polypeptide antibiotic, and wherein the absence of said first defined peak indicates the absence of a bacterium resistant to a cyclic cationic polypeptide antibiotic. This method is also used in a screening method to identify an inhibitor of cyclic cationic polypeptide antibiotic resistance in a bacterium. The matrix solution can contain 2,5-dihydroxybenzoic acid and aids in the selective extraction, co-crystallisation and ionisation of native Lipid A and/or modified Lipid A as an integral part of a bacterial membrane.

PATENT

Larrouy-Maumus G, 2018, Lipids as biomarkers of cancer and bacterial infections., Curr Med Chem

Lipids are ubiquitous molecules, known to play important roles in various cellular processes. Alterations to the lipidome can therefore be used as a read-out of the signs of disease, highlighting the importance to consider lipids as biomarkers in addition of nucleic acid and proteins. This mini-review exposes the current knowledge and limitations of the use of lipids as biomarkers of the top global killers which are cancer and bacterial infections.

JOURNAL ARTICLE

Rebollo-Ramirez S, Krokowski S, Lobato-Marquez D, Thomson M, Pennisi I, Mostowy S, Larrouy-Maumus Get al., 2018, Intact Cell Lipidomics Reveal Changes to the Ratio of Cardiolipins to Phosphatidylinositols in Response to Kanamycin in HeLa and Primary Cells, CHEMICAL RESEARCH IN TOXICOLOGY, Vol: 31, Pages: 688-696, ISSN: 0893-228X

JOURNAL ARTICLE

Rebollo-Ramirez S, Thomson M, Larrouy-Maumus G, 2018, The cyclic AMP receptor protein (CRP) orchestrates the increase in intracellular levels of cAMP in response to osmotic stress in pathogenic mycobacteria, Publisher: WILEY, Pages: 96-96, ISSN: 2211-5463

CONFERENCE PAPER

Rycroft JA, Gollan B, Grabe GJ, Hall A, Cheverton AM, Larrouy-Maumus G, Hare SA, Helaine Set al., 2018, Activity of acetyltransferase toxins involved in Salmonella persister formation during macrophage infection, NATURE COMMUNICATIONS, Vol: 9, ISSN: 2041-1723

JOURNAL ARTICLE

Berger CN, Crepin VF, Roumeliotis TI, Wright JC, Carson D, Pevsner-Fischer M, Furniss RCD, Dougan G, Dori-Bachash M, Yu L, Clements A, Collins JW, Elinav E, Larrouy-Maumus GJ, Choudhary JS, Frankel Get al., 2017, Citrobacter rodentium Subverts ATP Flux and Cholesterol Homeostasis in Intestinal Epithelial Cells In Vivo, CELL METABOLISM, Vol: 26, Pages: 738-+, ISSN: 1550-4131

JOURNAL ARTICLE

Robert CB, Thomson M, Vercellone A, Gardner F, Ernst RK, Larrouy-Maumus G, Nigou Jet al., 2017, Mass spectrometry analysis of intact Francisella bacteria identifies lipid A structure remodeling in response to acidic pH stress, BIOCHIMIE, Vol: 141, Pages: 16-20, ISSN: 0300-9084

JOURNAL ARTICLE

Buchieri MV, Cimino M, Rebollo-Ramirez S, Beauvineau C, Cascioferro A, Favre-Rochex S, Helynck O, Naud-Martin D, Larrouy-Maumus G, Munier-Lehmann H, Gicquel Bet al., 2017, Nitazoxanide Analogs Require Nitroreduction for Antimicrobial Activity in Mycobacterium smegmatis, JOURNAL OF MEDICINAL CHEMISTRY, Vol: 60, Pages: 7425-7433, ISSN: 0022-2623

JOURNAL ARTICLE

Pacholarz KJ, Burnley RJ, Jowitt TA, Ordsmith V, Pisco JP, Porrini M, Larrouy-Maumus G, Garlish RA, Taylor RJ, de Carvalho LPS, Barran PEet al., 2017, Hybrid Mass Spectrometry Approaches to Determine How L-Histidine Feedback Regulates the Enzyzme MtATP-Phosphoribosyltransferase, STRUCTURE, Vol: 25, Pages: 730-+, ISSN: 0969-2126

JOURNAL ARTICLE

Larrouy-Maumus G, Layre E, Clark S, Prandi J, Rayner E, Lepore M, de Libero G, Williams A, Puzo G, Gilleron Met al., 2017, Protective efficacy of a lipid antigen vaccine in a guinea pig model of tuberculosis, VACCINE, Vol: 35, Pages: 1395-1402, ISSN: 0264-410X

JOURNAL ARTICLE

Sirianni A, Krokowski S, Lobato-Marquez D, Buranyi S, Pfanzelter J, Galea D, Willis A, Culley S, Henriques R, Larrouy-Maumus G, Hollinshead M, Sancho-Shimizu V, Way M, Mostowy Set al., 2016, Mitochondria mediate septin cage assembly to promote autophagy of Shigella, EMBO REPORTS, Vol: 17, Pages: 1029-1043, ISSN: 1469-221X

JOURNAL ARTICLE

Larrouy-Maumus G, Marino LB, Madduri AVR, Ragan TJ, Hunt DM, Bassano L, Gutierrez MG, Moody DB, Pavan FR, de Carvalho LPSet al., 2016, Cell-Envelope Remodeling as a Determinant of Phenotypic Antibacterial Tolerance in Mycobacterium tuberculosis, ACS INFECTIOUS DISEASES, Vol: 2, Pages: 352-360, ISSN: 2373-8227

JOURNAL ARTICLE

Larrouy-Maumus G, Clements A, Filloux A, McCarthy RR, Mostowy Set al., 2016, Direct detection of lipid A on intact Gram-negative bacteria by MALDI-TOF mass spectrometry, JOURNAL OF MICROBIOLOGICAL METHODS, Vol: 120, Pages: 68-71, ISSN: 0167-7012

JOURNAL ARTICLE

Larrouy-Maumus G, Gilleron M, Skovierova H, Zuberogoitia S, Brennan PJ, Puzo G, Jackson M, Nigou Jet al., 2015, A glycomic approach reveals a new mycobacterial polysaccharide, GLYCOBIOLOGY, Vol: 25, Pages: 1163-1171, ISSN: 0959-6658

JOURNAL ARTICLE

Larrouy-Maumus G, 2015, Cholesterol acquisition by Mycobacterium tuberculosis, VIRULENCE, Vol: 6, Pages: 412-413, ISSN: 2150-5594

JOURNAL ARTICLE

Wheat WH, Dhouib R, Angala SK, Larrouy-Maumus G, Dobos K, Nigou J, Spencer JS, Jackson Met al., 2015, The presence of a galactosamine substituent on the arabinogalactan of Mycobacterium tuberculosis abrogates full maturation of human peripheral blood monocyte-derived dendritic cells and increases secretion of IL-10, TUBERCULOSIS, Vol: 95, Pages: 476-489, ISSN: 1472-9792

JOURNAL ARTICLE

Larrouy-Maumus G, Puzo G, 2015, Mycobacterial envelope lipids fingerprint from direct MALDI-TOF MS analysis of intact bacilli, TUBERCULOSIS, Vol: 95, Pages: 75-85, ISSN: 1472-9792

JOURNAL ARTICLE

Belardinelli JM, Larrouy-Maumus G, Jones V, de Carvalho LPS, McNeil MR, Jackson Met al., 2014, Biosynthesis and Translocation of Unsulfated Acyltrehaloses in Mycobacterium tuberculosis, Journal of Biological Chemistry, Vol: 289, Pages: 27952-27965, ISSN: 1083-351X

JOURNAL ARTICLE

Prosser GA, Larrouy-Maumus G, de Carvalho LPS, 2014, Metabolomic strategies for the identification of new enzyme functions and metabolic pathways, EMBO REPORTS, Vol: 15, Pages: 657-669, ISSN: 1469-221X

JOURNAL ARTICLE

Gouzy A, Larrouy-Maumus G, Wu T-D, Peixoto A, Levillain F, Lugo-Villarino G, Gerquin-Kern J-L, de Carvalho LPS, Poquet Y, Neyrolles Oet al., 2014, Mycobacterium tuberculosis nitrogen assimilation and host colonization require aspartate (vol 9, pg 674, 2013), NATURE CHEMICAL BIOLOGY, Vol: 10, Pages: 164-164, ISSN: 1552-4450

JOURNAL ARTICLE

Gouzy A, Larrouy-Maumus G, Bottai D, Levillain F, Dumas A, Wallach JB, Caire-Brandli I, de Chastellier C, Wu T-D, Poincloux R, Brosch R, Guerquin-Kern J-L, Schnappinger D, de Carvalho LPS, Poquet Y, Neyrolles Oet al., 2014, Mycobacterium tuberculosis Exploits Asparagine to Assimilate Nitrogen and Resist Acid Stress during Infection, PLOS PATHOGENS, Vol: 10, ISSN: 1553-7366

JOURNAL ARTICLE

Larrouy-Maumus G, Kelly G, de Carvalho LPS, 2014, Chemical Mechanism of Glycerol 3-Phosphate Phosphatase: pH-Dependent Changes in the Rate-Limiting Step, BIOCHEMISTRY, Vol: 53, Pages: 143-151, ISSN: 0006-2960

JOURNAL ARTICLE

Gouzy A, Larrouy-Maumus G, Wu T-D, Peixoto A, Levillain F, Lugo-Villarino G, Guerquin-Kern J-L, de Carvalho LPS, Poquet Y, Neyrolles Oet al., 2013, Mycobacterium tuberculosis nitrogen assimilation and host colonization require aspartate, NATURE CHEMICAL BIOLOGY, Vol: 9, Pages: 674-+, ISSN: 1552-4450

JOURNAL ARTICLE

Larrouy-Maumus G, Biswas T, Hunt DM, Kelly G, Tsodikov OV, de Carvalho LPSet al., 2013, Discovery of a glycerol 3-phosphate phosphatase reveals glycerophospholipid polar head recycling in Mycobacterium tuberculosis, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 110, Pages: 11320-11325, ISSN: 0027-8424

JOURNAL ARTICLE

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