Imperial College London

DrGeraldLarrouy-Maumus

Faculty of Natural SciencesDepartment of Life Sciences

Lecturer in Molecular Microbiology
 
 
 
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Contact

 

+44 (0)20 7594 7463g.larrouy-maumus

 
 
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Location

 

3.42Flowers buildingSouth Kensington Campus

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Summary

 

Publications

Publication Type
Year
to

36 results found

Lobato-Márquez D, Krokowski S, Sirianni A, Larrouy-Maumus G, Mostowy Set al., 2018, A requirement for septins and the autophagy receptor p62 in the proliferation of intracellular Shigella., Cytoskeleton (Hoboken)

Shigella flexneri, a Gram-negative enteroinvasive pathogen, causes inflammatory destruction of the human intestinal epithelium. During infection of epithelial cells, Shigella escape from the phagosome to the cytosol, where they reroute host cell glycolysis to obtain nutrients for proliferation. Septins, a poorly understood component of the cytoskeleton, can entrap cytosolic Shigella targeted to autophagy in cage-like structures to restrict bacterial proliferation. Although bacterial entrapment by septin caging has been the subject of intense investigation, the role of septins and the autophagy machinery in the proliferation of non-caged Shigella is mostly unknown. Here, we found that intracellular Shigella fail to efficiently proliferate in SEPT2-, SEPT7- or p62/SQSTM1-depleted cells. Consistent with a failure to proliferate, single cell analysis of bacteria not entrapped in septin cages showed that the number of metabolically active Shigella in septin- or p62-depleted cells is reduced. Targeted metabolomic analysis revealed that host cell glycolysis is dysregulated in septin-depleted cells, suggesting a key role for septins in modulation of glycolysis. Together, these results suggest that septins and the autophagy machinery may regulate metabolic pathways that promote the proliferation intracellular Shigella not entrapped in septin cages. This article is protected by copyright. All rights reserved.

JOURNAL ARTICLE

Rebollo-Ramirez S, Krokowski S, Lobato-Márquez D, Thomson M, Pennisi I, Mostowy S, Larrouy-Maumus Get al., 2018, Intact Cell Lipidomics Reveal Changes to the Ratio of Cardiolipins to Phosphatidylinositols in Response to Kanamycin in HeLa and Primary Cells., Chem Res Toxicol

Antimicrobial resistance is a major threat the world is currently facing. Development of new antibiotics and the assessment of their toxicity represent important challenges. Current methods for addressing antibiotic toxicity rely on measuring mitochondrial damage using ATP and/or membrane potential as a readout. In this study, we propose an alternative readout looking at changes in the lipidome on intact and unprocessed cells by matrix-assisted laser desorption ionization mass spectrometry. As a proof of principle, we evaluated the impact of known antibiotics (levofloxacin, ethambutol, and kanamycin) on the lipidome of HeLa cells and mouse bone marrow-derived macrophages. Our methodology revealed that clinically relevant concentrations of kanamycin alter the ratio of cardiolipins to phosphatidylinositols. Unexpectedly, only kanamycin had this effect even though all antibiotics used in this study led to a decrease in the maximal mitochondrial respiratory capacity. Altogether, we report that intact cell-targeted lipidomics can be used as a qualitative method to rapidly assess the toxicity of aminoglycosides in HeLa and primary cells. Moreover, these results demonstrate there is no direct correlation between the ratio of cardiolipins to phosphatidylinositols and the maximal mitochondrial respiratory capacity.

JOURNAL ARTICLE

Rebollo-Ramirez S, Thomson M, Larrouy-Maumus G, 2018, The cyclic AMP receptor protein (CRP) orchestrates the increase in intracellular levels of cAMP in response to osmotic stress in pathogenic mycobacteria, Publisher: WILEY, Pages: 96-96, ISSN: 2211-5463

CONFERENCE PAPER

Rycroft JA, Gollan B, Grabe GJ, Hall A, Cheverton AM, Larrouy-Maumus G, Hare SA, Helaine Set al., 2018, Activity of acetyltransferase toxins involved in Salmonella persister formation during macrophage infection, NATURE COMMUNICATIONS, Vol: 9, ISSN: 2041-1723

JOURNAL ARTICLE

Berger CN, Crepin VF, Roumeliotis TI, Wright JC, Carson D, Pevsner-Fischer M, Furniss RCD, Dougan G, Dori-Bachash M, Yu L, Clements A, Collins JW, Elinav E, Larrouy-Maumus GJ, Choudhary JS, Frankel Get al., 2017, Citrobacter rodentium Subverts ATP Flux and Cholesterol Homeostasis in Intestinal Epithelial Cells In Vivo, CELL METABOLISM, Vol: 26, Pages: 738-+, ISSN: 1550-4131

JOURNAL ARTICLE

Buchieri MV, Cimino M, Rebollo-Ramirez S, Beauvineau C, Cascioferro A, Favre-Rochex S, Helynck O, Naud-Martin D, Larrouy-Maumus G, Munier-Lehmann H, Gicquel Bet al., 2017, Nitazoxanide Analogs Require Nitroreduction for Antimicrobial Activity in Mycobacterium smegmatis, JOURNAL OF MEDICINAL CHEMISTRY, Vol: 60, Pages: 7425-7433, ISSN: 0022-2623

JOURNAL ARTICLE

Larrouy-Maumus G, Layre E, Clark S, Prandi J, Rayner E, Lepore M, de Libero G, Williams A, Puzo G, Gilleron Met al., 2017, Protective efficacy of a lipid antigen vaccine in a guinea pig model of tuberculosis, VACCINE, Vol: 35, Pages: 1395-1402, ISSN: 0264-410X

JOURNAL ARTICLE

Pacholarz KJ, Burnley RJ, Jowitt TA, Ordsmith V, Pisco JP, Porrini M, Larrouy-Maumus G, Garlish RA, Taylor RJ, de Carvalho LPS, Barran PEet al., 2017, Hybrid Mass Spectrometry Approaches to Determine How L-Histidine Feedback Regulates the Enzyzme MtATP-Phosphoribosyltransferase, STRUCTURE, Vol: 25, Pages: 730-+, ISSN: 0969-2126

JOURNAL ARTICLE

Robert CB, Thomson M, Vercellone A, Gardner F, Ernst RK, Larrouy-Maumus G, Nigou Jet al., 2017, Mass spectrometry analysis of intact Francisella bacteria identifies lipid A structure remodeling in response to acidic pH stress, BIOCHIMIE, Vol: 141, Pages: 16-20, ISSN: 0300-9084

JOURNAL ARTICLE

Larrouy-Maumus G, Clements A, Filloux A, McCarthy RR, Mostowy Set al., 2016, Direct detection of lipid A on intact Gram-negative bacteria by MALDI-TOF mass spectrometry, JOURNAL OF MICROBIOLOGICAL METHODS, Vol: 120, Pages: 68-71, ISSN: 0167-7012

JOURNAL ARTICLE

Larrouy-Maumus G, Marino LB, Madduri AVR, Ragan TJ, Hunt DM, Bassano L, Gutierrez MG, Moody DB, Pavan FR, de Carvalho LPSet al., 2016, Cell-Envelope Remodeling as a Determinant of Phenotypic Antibacterial Tolerance in Mycobacterium tuberculosis, ACS INFECTIOUS DISEASES, Vol: 2, Pages: 352-360, ISSN: 2373-8227

JOURNAL ARTICLE

Sirianni A, Krokowski S, Lobato-Marquez D, Buranyi S, Pfanzelter J, Galea D, Willis A, Culley S, Henriques R, Larrouy-Maumus G, Hollinshead M, Sancho-Shimizu V, Way M, Mostowy Set al., 2016, Mitochondria mediate septin cage assembly to promote autophagy of Shigella, EMBO REPORTS, Vol: 17, Pages: 1029-1043, ISSN: 1469-221X

JOURNAL ARTICLE

Larrouy-Maumus G, 2015, Cholesterol acquisition by Mycobacterium tuberculosis, VIRULENCE, Vol: 6, Pages: 412-413, ISSN: 2150-5594

JOURNAL ARTICLE

Larrouy-Maumus G, Gilleron M, Skovierova H, Zuberogoitia S, Brennan PJ, Puzo G, Jackson M, Nigou Jet al., 2015, A glycomic approach reveals a new mycobacterial polysaccharide, GLYCOBIOLOGY, Vol: 25, Pages: 1163-1171, ISSN: 0959-6658

JOURNAL ARTICLE

Larrouy-Maumus G, Puzo G, 2015, Mycobacterial envelope lipids fingerprint from direct MALDI-TOF MS analysis of intact bacilli, TUBERCULOSIS, Vol: 95, Pages: 75-85, ISSN: 1472-9792

JOURNAL ARTICLE

Wheat WH, Dhouib R, Angala SK, Larrouy-Maumus G, Dobos K, Nigou J, Spencer JS, Jackson Met al., 2015, The presence of a galactosamine substituent on the arabinogalactan of Mycobacterium tuberculosis abrogates full maturation of human peripheral blood monocyte-derived dendritic cells and increases secretion of IL-10, TUBERCULOSIS, Vol: 95, Pages: 476-489, ISSN: 1472-9792

JOURNAL ARTICLE

Belardinelli JM, Larrouy-Maumus G, Jones V, de Carvalho LPS, McNeil MR, Jackson Met al., 2014, Biosynthesis and Translocation of Unsulfated Acyltrehaloses in Mycobacterium tuberculosis, Journal of Biological Chemistry, Vol: 289, Pages: 27952-27965, ISSN: 1083-351X

JOURNAL ARTICLE

Gouzy A, Larrouy-Maumus G, Bottai D, Levillain F, Dumas A, Wallach JB, Caire-Brandli I, de Chastellier C, Wu T-D, Poincloux R, Brosch R, Guerquin-Kern J-L, Schnappinger D, de Carvalho LPS, Poquet Y, Neyrolles Oet al., 2014, Mycobacterium tuberculosis Exploits Asparagine to Assimilate Nitrogen and Resist Acid Stress during Infection, PLOS PATHOGENS, Vol: 10, ISSN: 1553-7366

JOURNAL ARTICLE

Larrouy-Maumus G, Kelly G, de Carvalho LPS, 2014, Chemical Mechanism of Glycerol 3-Phosphate Phosphatase: pH-Dependent Changes in the Rate-Limiting Step, BIOCHEMISTRY, Vol: 53, Pages: 143-151, ISSN: 0006-2960

JOURNAL ARTICLE

Prosser GA, Larrouy-Maumus G, de Carvalho LPS, 2014, Metabolomic strategies for the identification of new enzyme functions and metabolic pathways, EMBO REPORTS, Vol: 15, Pages: 657-669, ISSN: 1469-221X

JOURNAL ARTICLE

Gouzy A, Larrouy-Maumus G, Wu T-D, Peixoto A, Levillain F, Lugo-Villarino G, Guerquin-Kern J-L, de Carvalho LPS, Poquet Y, Neyrolles Oet al., 2013, Mycobacterium tuberculosis nitrogen assimilation and host colonization require aspartate, NATURE CHEMICAL BIOLOGY, Vol: 9, Pages: 674-+, ISSN: 1552-4450

JOURNAL ARTICLE

Larrouy-Maumus G, Biswas T, Hunt DM, Kelly G, Tsodikov OV, de Carvalho LPSet al., 2013, Discovery of a glycerol 3-phosphate phosphatase reveals glycerophospholipid polar head recycling in Mycobacterium tuberculosis, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 110, Pages: 11320-11325, ISSN: 0027-8424

JOURNAL ARTICLE

Mao C, Shukla M, Larrouy-Maumus G, Dix FL, Kelley LA, Sternberg MJ, Sobral BW, de Carvalho LPSet al., 2013, Functional assignment of Mycobacterium tuberculosis proteome revealed by genome-scale fold-recognition, TUBERCULOSIS, Vol: 93, Pages: 40-46, ISSN: 1472-9792

JOURNAL ARTICLE

Jackson M, Dhouib R, Skovierova H, Larrouy-Maumus G, Angala SK, Wheat WH, Ha P, Gilleron M, Brennan PJ, Puzo G, Nigou Jet al., 2012, Biogenesis of mycobacterial cell envelope glycoconjugates, Experimental Biology Meeting, Publisher: FEDERATION AMER SOC EXP BIOL, ISSN: 0892-6638

CONFERENCE PAPER

Larrouy-Maumus G, Skovierova H, Dhouib R, Angala SK, Zuberogoitia S, Ha P, Villela AD, Mikusova K, Noguera A, Gilleron M, Valentinova L, Kordulakova J, Brennan PJ, Puzo G, Nigou J, Jackson Met al., 2012, A Small Multidrug Resistance-like Transporter Involved in the Arabinosylation of Arabinogalactan and Lipoarabinomannan in Mycobacteria, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 287, ISSN: 0021-9258

JOURNAL ARTICLE

Pedreno S, Pisco JP, Larrouy-Maumus G, Kelly G, de Carvalho LPSet al., 2012, Mechanism of Feedback Allosteric Inhibition of ATP Phosphoribosyltransferase, BIOCHEMISTRY, Vol: 51, Pages: 8027-8038, ISSN: 0006-2960

JOURNAL ARTICLE

Cot M, Ray A, Gilleron M, Vercellone A, Larrouy-Maumus G, Armau E, Gauthier S, Tiraby G, Puzo G, Nigou Jet al., 2011, Lipoteichoic Acid in Streptomyces hygroscopicus: Structural Model and Immunomodulatory Activities, PLOS ONE, Vol: 6, ISSN: 1932-6203

JOURNAL ARTICLE

Krishna S, Ray A, Dubey SK, Larrouy-Maumus G, Chalut C, Castanier R, Noguera A, Gilleron M, Puzo G, Vercellone A, Nampoothiri KM, Nigou Jet al., 2011, Lipoglycans Contribute to Innate Immune Detection of Mycobacteria, PLOS ONE, Vol: 6, ISSN: 1932-6203

JOURNAL ARTICLE

Layre E, Cala-De Paepe D, Larrouy-Maumus G, Vaubourgeix J, Mundayoor S, Lindner B, Puzo G, Gilleron Met al., 2011, Deciphering sulfoglycolipids of Mycobacterium tuberculosis, JOURNAL OF LIPID RESEARCH, Vol: 52, Pages: 1098-1110, ISSN: 0022-2275

JOURNAL ARTICLE

Brodin P, Poquet Y, Levillain F, Peguillet I, Larrouy-Maumus G, Gilleron M, Ewann F, Christophe T, Fenistein D, Jang J, Jang M-S, Park S-J, Rauzier J, Carralot J-P, Shrimpton R, Genovesio A, Gonzalo-Asensio JA, Puzo G, Martin C, Brosch R, Stewart GR, Gicquel B, Neyrolles Oet al., 2010, High Content Phenotypic Cell-Based Visual Screen Identifies Mycobacterium tuberculosis Acyltrehalose-Containing Glycolipids Involved in Phagosome Remodeling, PLOS PATHOGENS, Vol: 6, ISSN: 1553-7366

JOURNAL ARTICLE

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