Imperial College London
Alternate

DrGeraldLarrouy-Maumus

Faculty of Natural Sciences, Department of Life Sciences

Reader in Molecular Microbiology
 
 
 
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Contact

 

+44 (0)20 7594 7463g.larrouy-maumus

 
 
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Location

 

3.42Flowers buildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@misc{Filloux:2018,
author = {Filloux, A and Larrouy-Maumus, G and Dortet, L},
title = {Method of detection: Use of Lipid A and its modifications as a direct detection of antimicrobials resistance},
type = {Patent},
url = {https://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20180907&DB=&locale=en_EP&CC=WO&NR=2018158573A1&KC=A1&ND=4#},
year = {2018}
}
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RIS format (EndNote, RefMan)

TY  - PAT
AB  - The present invention is directed to a method for detecting the presence or absence of a bacterium resistant to a cyclic cationic polypeptide antibiotic, comprising: (a) subjecting a test sample to mass spectrometry analysis and generating a mass spectrum output; wherein said test sample comprises a bacterial membrane or a fragment thereof, wherein the fragment comprises a non- Lipid A component; (b) identifying in said mass spectrum output a first defined peak indicative of the presence of Lipid A modified by phosphoethanolamine, wherein said first defined peak is a peak present in a mass spectrum output for Lipid A modified by phosphoethanolamine and wherein said first defined peak is absent from a corresponding mass spectrum output for native Lipid A; and (c) wherein the presence of said first defined peak indicates the presence of a bacterium resistant to a cyclic cationic polypeptide antibiotic, and wherein the absence of said first defined peak indicates the absence of a bacterium resistant to a cyclic cationic polypeptide antibiotic. This method is also used in a screening method to identify an inhibitor of cyclic cationic polypeptide antibiotic resistance in a bacterium. The matrix solution can contain 2,5-dihydroxybenzoic acid and aids in the selective extraction, co-crystallisation and ionisation of native Lipid A and/or modified Lipid A as an integral part of a bacterial membrane.
AU  - Filloux,A
AU  - Larrouy-Maumus,G
AU  - Dortet,L
PY  - 2018///
TI  - Method of detection: Use of Lipid A and its modifications as a direct detection of antimicrobials resistance
UR  - https://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20180907&DB=&locale=en_EP&CC=WO&NR=2018158573A1&KC=A1&ND=4#
ER  -
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